Curator Dashboard

ddPCR Analysis tool

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• 1 year ago - submitted by Nicole Reddy

Triple-labeling Multiplex Immunofluorescence Kit

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• 1 year ago - submitted by Yujing Zhong

Disodium clodronate is a selective macrophage-killing agent that removes macrophages from tissues by inducing apoptosis. Currently, the ideal reagent on the market for efficient macrophage depletion is disodium clodronate liposomes. The clodronate molecules are encapsulated within liposomal vesicles, offering high activity, enhanced stability, and ease of use. Intravenous injection of 0.2 mL clodronate liposomes can achieve an 80-90% clearance rate of macrophages in the spleen (red pulp macrophages) within 24 hours. A version of this product without disodium clodronate is also available, with the liposomes containing only PBS, which can be used as a control in experiments to determine if macrophage depletion is specifically due to the effects of clodronate liposome injection.

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• 1 year ago - submitted by Yujing Zhong

Clodronate Liposomes were developed by Professor Nico van Rooijen at Vrije University Amsterdam, Netherlands. They utilize the phagocytic mechanism of macrophages to deliver the membrane-impermeable clodronate into cells. Within macrophages, clodronate dissolved in the aqueous phase of the liposomes is released under the action of lysosomal phosphatases and accumulates inside the cell. When clodronate reaches a certain concentration, it induces apoptosis in macrophages, thereby achieving the goal of macrophage depletion. Clodronate Liposomes are suitable for various injection routes, including intravenous, intraperitoneal, subcutaneous, intranasal, and testicular injections. The injection dosage depends on the mouse’s body weight, injection schedule, route of administration, and the specific experimental objectives.

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• 1 year ago - submitted by Yujing Zhong

Zombie Aqua™ is an amine-reactive fluorescent dye that is non-permeant to live cells but permeant to cells with compromised membranes. Thus, it can be used to assess live vs. dead status of mammalian cells. Zombie Aqua™ is a polar, water-soluble dye providing very bright green fluorescence, making it suitable for use in multi-color detection.

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• 1 year ago - submitted by Yujing Zhong

for nucleic acid staining

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• 1 year ago - submitted by Yujing Zhong

The Thermo Scientific PageRuler Prestained Protein Ladder is a protein size marker for SDS-PAGE and Western blotting, consisting of a mixture of ten proteins (10 to 180 kDa) that are prestained in blue, orange, and green. The protein ladder is provided in a ready-to-use format and can be directly loaded onto gels without the need for heating, reduction, or the addition of sample buffer prior to use.

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• 1 year ago - submitted by Yujing Zhong

a Wnt pathway agonist

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• 1 year ago - submitted by Yujing Zhong

a Wnt pathway inhibitor

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• 1 year ago - submitted by Yujing Zhong

NF-kB pathway inhibitor

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• 1 year ago - submitted by Yujing Zhong

recombinant murine MSLN protein produced by Novoprotein

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• 1 year ago - submitted by Yujing Zhong

This 1X Red Blood Cell (RBC) Lysis Buffer is formulated to optimally lyse red blood cells in single-cell suspensions from hematopoietic tissues of mice, such as the spleen, and human peripheral blood. This buffer contains ammonium chloride, and when used according to the specified procedure, it effectively lyses red blood cells while minimizing the impact on lymphocytes. Nucleated red blood cells are not effectively lysed by ammonium chloride

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• 1 year ago - submitted by Yujing Zhong

The Applied Biosystems QuantStudio real-time PCR system can detect extremely small changes in gene expression, as little as 1.5-fold, which might be overlooked by other systems. Its dynamic range of up to 10 logs allows for tracking a wide range of expression changes across various sample types and conditions.

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• 1 year ago - submitted by Yujing Zhong

FLUOstar® Omega is a multi-mode microplate reader with three detection modes. It utilizes ultra-fast UV/Visible spectroscopy or filters, along with high-sensitivity filters for all other detection modes. The FLUOstar Omega is an ideal microplate reader for applications in the life sciences field.

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• 1 year ago - submitted by Yujing Zhong

Mesothelin, also known as MSLN or MPF, is a protein encoded by the MSLN gene in humans. This gene is located at 16p13.3. It encodes a precursor protein that is cleaved into two products: megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor is a cytokine that stimulates the formation of megakaryocyte colonies in the bone marrow. Mesothelin is a glycosylphosphatidylinositol-anchored cell surface protein that acts as a cell adhesion protein. This protein is overexpressed in epithelial mesothelioma, ovarian cancer, and certain squamous cell carcinomas. This kit is intended for in vitro qualitative or quantitative analysis and is for research use only, not for clinical diagnostics. It is suitable for serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids.

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• 1 year ago - submitted by Yujing Zhong

The Steriflip filter unit is designed for use with 50 mL centrifuge tubes, eliminating the need for transferring samples. To filter, simply connect the 50 mL centrifuge tube containing the sample to the Steriflip unit, invert it, and apply suction. Once the filtration is complete, the filtrate is collected in the 50 mL centrifuge tube attached below.

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• 1 year ago - submitted by Yujing Zhong

The Crystal Violet Staining Solution produced by BiYunTian is a commonly used staining solution for tissue or cell staining that can color cell nuclei deep purple. Crystal violet is a basic dye that can bind to the DNA in cell nuclei, resulting in nuclear staining. A 100 ml package of this staining solution can stain at least 200 samples, while a 500 ml package can stain at least 1,000 samples.

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• 1 year ago - submitted by Yujing Zhong

SeaPlaque® Agarose, 25 g, is the original low melting temperature agarose.

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• 1 year ago - submitted by Yujing Zhong

The BioTek Synergy HTX is a compact, economical multimode reader for 6- to 384-well microplates and Take3 microvolume plates. The unique dual optics design provides superior performance for UV-Vis absorbance, fluorescence, luminescence, and AlphaScreen/AlphaLISA workflows. With incubation and shaking, plus a dual reagent injector module, the system will meet all your assay requirements now and in the future. Synergy HTX is controlled by the easy-to-use, yet powerful, Gen5 software for data collection, analysis, exporting, and reporting.

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• 1 year ago - submitted by Yujing Zhong

Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing Dojindo’s highly water-soluble tetrazolium salt. WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator. CCK-8 is a one-bottle solution; no premixing of components is required. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells.

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• 1 year ago - submitted by Yujing Zhong