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Diphtheria toxin may be used as a toxin element in the construction of immunotoxin for cell specific cytotoxicity. The translocation domain of bacterial toxins with natural endosome escape mechanism has been used in the development of efficient nonviral vectors for applications in gene therapy.

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• 4 years ago - submitted by Kaira Church

Streptozocin or streptozotocin, is obtained from Streptomyces achromogenes. Streptozotocin, a glucosamine-nitrosourea, is a DNA alkylating agent that enters cells exclusively via the GLUT2 glucose transport protein. Streptozocin, a diabetagen, is especially toxic to pancreatic islet insulin-producing β-cells. It is toxic to GLUT2 positive neuroendocrine tumor cells.

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• 4 years ago - submitted by Kaira Church

Tamoxifen is a selective estrogen response modifier (SERM), protein kinase C inhibitor and anti-angiogenetic factor. Tamoxifen is a prodrug that is metabolized to active metabolites 4-hydroxytamoxifen (4-OHT) and endoxifen by cytochrome P450 isoforms CYP2D6 and CYP3A4. In breast cancer, the gene repressor activity of tamoxifen against ERBB2 is dependent upon PAX2. Blocks estradiol-stimulated VEGF production in breast tumor cells

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• 4 years ago - submitted by Kaira Church

Rabbit Anti-LPP,polyclonal Antibody.

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• SciCrunch
• 4 years ago - submitted by xiaoniu he

Rabbit Anti-LPP polyclonal Antibody

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• SciCrunch
• 4 years ago - submitted by xiaoniu he

KLH conjugated Synthetic peptide corresponding to Mouse BrdU

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• 4 years ago - submitted by shenghui liu

Software pipeline for creating harmonized single cell RNA-seq dataset for retina with publicly available data. Platform for analysis of single cell eye in disk. Snakefile based process to turn 1.4 million ocular cells into unified meta-atlas. Meta-atlas that compiles 1.2 million single-cell back of the eye transcriptomes across studies, publications, and species.

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• SciCrunch
• 4 years ago - submitted by David McGaughey

Whippet it is a bioinformatic tool to quantify alternative splicing events, intron retention and circRNA isoforms from RNAseq data. easy-to-use RNA-seq analysis method that rapidly-with hardware requirements compatible with a laptop-models and quantifies AS events of any complexity without loss of accuracy. Using an entropic measure of splicing complexity, Whippet reveals that one-third of human protein coding genes produce transcripts with complex AS events involving co-expression of two or more principal splice isoforms. We observe that high-entropy AS events are more prevalent in tumor relative to matched normal tissues and correlate with increased expression of proto-oncogenic splicing factors. Whippet thus affords the rapid and accurate analysis of AS events of any complexity, and as such will facilitate future biomedical research.

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• 4 years ago - by Anonymous

MultiVision AP and HRP polymers are innovative, patented technologies consisting of smaller amino acid polymer subunits that minimize conflicts in binding the target protein. The smaller polymer allows for decreased binding conflicts, resulting in more consistent staining and better signal amplification. A primary antibody cocktail of choice may be used in conjunction with ready-to-use MultiVision polymer cocktail, composed of anti-mouse/ horseradish peroxidase (HRP) plus anti-rabbit/alkaline phosphatase (AP) Cocktail is specially formulated from MultiVision polymers that provide increased sensitivity, time-savings, and detection simplicity Yields higher sensitivity and antibody efficiency as well as better signal-to-noise ratios Biotin-free, eliminating background staining Antigen Specificity: anti-Mouse IgG (H+L) and anti-Rabbit IgG (H+L) Enzyme: Peroxidase and Phosphatase Chromogen/Substrate: LVBlue/ LVRe

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• 4 years ago - submitted by Thomas Emmanuel

Software package for using multi locus genotype data to investigate population structure. Used for inferring presence of distinct populations, assigning individuals to populations, studying hybrid zones, identifying migrants and admixed individuals, and estimating population allele frequencies in situations where many individuals are migrants or admixed. Can be applied to most of commonly used genetic markers, including SNPS, microsatellites, RFLPs and Amplified Fragment Length Polymorphisms.

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• 4 years ago - by Anonymous

Software application for automated quantification of behavior during social interaction in adult zebrafish. Software data extraction and analysis tool built in R language. Part of Argus project.

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• SciCrunch
• 4 years ago - submitted by Edyta Vieth

Open access high throughput pipeline and database for rodent touchscreen based cognitive assessment.Integration of touchscreen cognitive testing with open access database public repository as well as Web platform for knowledge dissemination.

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• SciCrunch
• 4 years ago - submitted by Edyta Vieth

Open access database for all cognitive data collected from touchscreen related tasks. Performs data comparison and interactive data visualization for any data uploaded onto the site. There are also guidelines and video tutorials available.

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• SciCrunch
• 4 years ago - submitted by Edyta Vieth

Public research university in Ann Arbor, Michigan.

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• RRID-Legacy
• 5 years ago - submitted by Edyta Vieth

Rabbit monoclonal [EPR10643(2)] to TMEM16A produced by abcam

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• SciCrunch
• 5 years ago - submitted by Tyler Rodriguez

The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant Human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds. The calculated molecular weight of Recombinant Human IGF-II is 7.5 kDa.

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• 5 years ago - submitted by Ki-Sun Park

This assay employs an antibody specific for mouse IGF2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGF2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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• 5 years ago - submitted by Ki-Sun Park

PD98059 is supplied as a lyophilized powder. For a 20 mM stock, reconstitute 1.5 mg in 280 μl anhydrous DMSO (5 mg in 933.3 μl anhydrous DMSO). For 50 mM stock, reconstitute 1.5 mg in 112 μl anhydrous DMSO (5 mg in 373.3 μl). Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used as a pretreatment at 5 - 50 μM for one hour prior to treating with a stimulator.

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• 5 years ago - submitted by Ki-Sun Park

BMS-754807 is an orally bioavailable antagonist of human insulin-like growth factor type I receptor (IGF-1R) with potential antineoplastic activity. BMS-7548077 binds to IGF-1R, preventing IGF-1 ligand binding and activation of IGF-1R-mediated signaling pathways; inhibition of IGF-1R-mediated signaling pathways may result in the inhibition of tumor cell proliferation and the induction of tumor cell apoptosis. IGF-1R is a receptor tyrosine kinase expressed on most tumor cells and is involved in mitogenesis, angiogenesis, and tumor cell survival

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• 5 years ago - submitted by Ki-Sun Park

RNAscope® 2.5 HD Detection Reagents-RED assay are designed for RNA in situ hybridization (ISH) based on ACD's patented signal amplification and background suppression technology. This manual assay is a single-plex, chromogenic-RED assay. The detection reagents contains AMP 1, APM2, AMP 3, AMP 4, AMP 5, AMP 6, Fast RED-A, Fast RED-B. The detection reagents provide enough reagents to stain ~20 tissue sections, each with an area of approximately 20 mm x 20 mm (0.75in x 0.75in).To run the detection reagents you will need the pretreatment kit and the wash buffer reagents.Disclaimer: Photos 2.5 products will be updated shortly.

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• 5 years ago - submitted by Ki-Sun Park