Curator Dashboard

Taq Pro Universal SYBR qPCR Master Mix

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• 1 year ago - submitted by Yujing Zhong

HiScript III RT SuperMix for qPCR (+gDNA wiper)

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• 1 year ago - submitted by Yujing Zhong

FastPureRNA Isolation Kit

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• 1 year ago - submitted by Yujing Zhong

CytoFLEX flow cytometer

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• 1 year ago - submitted by Yujing Zhong

Zombie Aqua™ is an amine-reactive fluorescent dye that is non-permeant to live cells but permeant to cells with compromised membranes. Thus, it can be used to assess live vs. dead status of mammalian cells. Zombie Aqua™ is a polar, water-soluble dye providing very bright green fluorescence, making it suitable for use in multi-color detection.

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• 1 year ago - submitted by Yujing Zhong

CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ PLUS is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors and is more effective than TruStain FcX™.

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• 1 year ago - submitted by Yujing Zhong

Falcon® 100 µm cell strainer is a sterile, easy-to-use device for rapidly isolating primary cells to consistently obtain a uniform single-cell suspension from tissues. Applications include stem cells and primary cells. Individually packaged, 50/case

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• 1 year ago - submitted by Yujing Zhong

Benchtop instrument for semi-automated dissociation of tissues into single-cell suspensions or thorough homogenates. Single sample or two samples in parallel can be processed. Two types of unique gentleMACS Tubes used with instrument enable time-saving and easy dissociation or homogenization of tissues in closed system. Instrument offers optimized gentleMACS Programs for variety of specific applications. Special protocols have been developed for various tissues

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• 1 year ago - submitted by Yujing Zhong

The C1.18.4 monoclonal antibody is ideal for use as a non-reactive isotype-matched control for mouse IgG2a antibodies in most in vivo and in vitro applications.

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• 1 year ago - submitted by Yujing Zhong

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

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• 1 year ago - submitted by Yujing Zhong

Ultra-Low Attachment surface with covalently bonded hydrogel that minimizes cell attachment, protein absorption, enzyme activation and cellular activation The surface is noncytotoxic, biologically inert and nondegradable Round bottoms with 330 µL total volume Recommended working volumes of 75 to 200 µL Nonreversable lids with condensation rings to reduce contamination Sterilized by gamma radiation and nonpyrogenic Individual alphanumeric codes for well identification Individually wrapped

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• 1 year ago - submitted by Yujing Zhong

The Foxp3 / Transcription Factor Staining Buffer Set has been formulated and optimized for staining with antibodies to transcription factors and nuclear proteins, such as FOXP3 and Ki-67, as well as cytokines and chemokines.

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• 1 year ago - submitted by Yujing Zhong

Brefeldin A (BFA) is a protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation. Especially useful for the intracellular staining of cytokines, BFA leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum (see Jung, et al., 1993). Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest (see references below for additional information). Brefeldin A Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.

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• 1 year ago - submitted by Yujing Zhong

Dynabeads™ Mouse T Activator CD3/CD28 provides a simple method for T cell activation and expansion without the need for feeder layer cells (antigen-presenting cells) or antigens. The 4.5 µm diameter inert superparamagnetic beads are similar in size to antigen-presenting cells and are covalently coupled with anti-CD3 and anti-CD28 antibodies. These two antibodies provide the primary signal and co-stimulatory signal, optimized for efficient T cell activation and expansion. Recombinant IL-2 can be used to stimulate the expansion of T cell populations. After activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet.

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• 1 year ago - submitted by Yujing Zhong

CFSE Cell Division Tracker Kit is composed of 5 vials, 100 µg per vial of CFSE (formally known as 5-(and 6)-Carboxyfluorescein diacetate succinimidyl ester of CFDA SE), and 500 µl of anhydrous DMSO. CFSE is able to passively diffuse into cells. Inside the cell, its acetate groups are cleaved by intracellular esterases, and the molecules are converted to fluorescent esters. CFSE is retained within the cell and covalently couples to intracellular molecules via its succinimidyl group. Due to this covalent coupling reaction, fluorescent CFSE can be retained within the cell for an extremely long period. Also, due to this stable linkage, once the dye has been incorporated within the cell, it is not transferred to adjacent cells. CFSE is widely used for cell proliferation assays and in vivo cell tracking.

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• 1 year ago - submitted by Yujing Zhong

Mouse CD8a (Ly-2) MicroBeads have been developed for positive selection or depletion of mouse CD8a+ T cells from single-cell suspensions of lymphoid and non-lymphoid tissues or from peripheral blood.

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• 1 year ago - submitted by Yujing Zhong

M-CSF, also known as CSF-1, is a four-α-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF protein is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF protein can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human M-CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

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• 1 year ago - submitted by Yujing Zhong

RPMI 1640 medium (Roswell Park Memorial Institute 1640 medium) was initially developed for the suspension culture of human leukemia monolayer cells. RPMI 1640 has been found to be suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMCs, astrocytes, and cancer cells.

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• 1 year ago - submitted by Yujing Zhong

Lipofectamine 3000 is a widely used transfection reagent primarily designed for the efficient delivery of nucleic acids (such as DNA and RNA) into cells. Here are some detailed descriptions of Lipofectamine 3000: Composition: Lipofectamine 3000 is a lipid-based transfection reagent that contains various cationic lipids and polymers, enabling the formation of complexes with nucleic acids to facilitate their penetration through the cell membrane. Transfection Efficiency: Compared to other transfection reagents, Lipofectamine 3000 generally exhibits higher transfection efficiency, effectively transfecting a wide range of cell types, including those that are typically difficult to transfect. Versatility: It is suitable for various applications, such as gene expression studies, RNA interference (RNAi), and CRISPR/Cas9 gene editing. It supports the transfection of different types of nucleic acids, including plasmid DNA, mRNA, and siRNA. Cell Types: Lipofectamine 3000 is effective for a variety of cells, including cell lines and primary cells, particularly demonstrating excellent performance in the transfection of mammalian cells. Optimization: The reagent provides a range of optimization conditions to help users adjust transfection efficiency based on specific cell types and experimental requirements. Usage Method: Typically, in the laboratory, Lipofectamine 3000 is mixed with nucleic acids to form lipid complexes, which are then added to the cell culture medium for transfection.

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• 1 year ago - submitted by Yujing Zhong

Hygromycin B is a broad-spectrum antibiotic primarily used for selective resistance and cell culture. It was originally isolated from the bacterium Streptomyces hygroscopicus. Here are some detailed descriptions of Hygromycin B: Mechanism of Action: Hygromycin B exerts its effects by interfering with bacterial protein synthesis. It binds to the ribosomal subunit, inhibiting the elongation of the peptide chain, which leads to a blockade of protein synthesis. Resistance Mechanism: Some bacteria and eukaryotic cells can develop resistance to Hygromycin B through specific genes (such as the hyg gene), allowing them to survive in environments where the antibiotic is present. Applications: Molecular Biology: Used for selecting cells that express specific resistance genes in transgenic plants and animal cells. Microbiology: Employed to select resistant bacteria for studying specific genetic backgrounds or resistance mechanisms. Cytotoxicity: Although Hygromycin B is widely used in cell culture, it has a certain level of cytotoxicity to mammalian cells, so careful concentration control is necessary when using it. Dosage and Usage: It is typically added to cell culture at specific concentrations (e.g., 50-400 µg/mL), depending on the cell type and experimental design. Overall, Hygromycin B is an important research tool widely used in molecular biology and cell biology.

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• 1 year ago - submitted by Yujing Zhong