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suitable for release of physiologically active rat hepatocytes, Type IV, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

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• 1 year ago - submitted by Yujing Zhong

TruSeq RNA Sample Preparation Kit

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• 1 year ago - submitted by Yujing Zhong

This enzyme randomly cleaves single-stranded or double-stranded DNA to produce deoxyribonuclease endonuclease with 5'-P terminal oligonucleotides. The enzyme has been purified to remove proteases and nucleases, which enhances its stability in the neutral pH range, making it suitable for RNA preparation. The main features of TRIzol reagent include: The ability to isolate RNA, DNA, and protein from the same sample. Excellent lysis capability even for difficult-to-handle sample types. Formulation and protocol optimization for tissues, cells, serum, viruses, and bacteria. Reliable purification of RNA from different sample sizes and sources TRIzol reagent performs well when processing small amounts of tissue (50–100 mg) and cells (5 × 10^6), as well as large amounts of tissue (≥1 g) and cells (>10^7). The provided protocols can be used for purifying samples from human, animal, plant, or bacterial sources. TRIzol reagent maintains RNA integrity due to its efficient inhibition of ribonuclease activity while disrupting cells and lysing cellular components during sample homogenization. The ease of use of TRIzol reagent allows for the simultaneous processing of multiple samples, with the entire process taking less than one hour. Total RNA isolated using TRIzol reagent is free from protein and DNA contamination. Formulation allows for multiple separations of various molecular targets TRIzol reagent enables the sequential precipitation of RNA, DNA, and protein from a single sample. After homogenizing the sample with TRIzol reagent, chloroform is added to separate the homogenate into a clear upper aqueous phase (containing RNA), an interphase, and a red lower organic phase (containing DNA and protein). RNA is precipitated from the aqueous phase using isopropanol, while DNA is precipitated from the interphase/organic phase using ethanol. Protein is precipitated from the phenol-ethanol supernatant using isopropanol. The precipitated RNA, DNA, or protein is washed to remove impurities and then resuspended for downstream applications.

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• 1 year ago - submitted by Yujing Zhong

TRIzol reagent is a complete, ready-to-use reagent that isolates high-quality total RNA or simultaneously separates RNA, DNA, and protein from various biological samples. This phenol and guanidine isothiocyanate monophasic solution can isolate RNA, DNA, and protein components from cell and tissue samples of humans, animals, plants, yeast, or bacteria within one hour. The main features of TRIzol reagent include: The ability to isolate RNA, DNA, and protein from the same sample. Excellent lysis capability even for difficult-to-handle sample types. Formulation and protocol optimization for tissues, cells, serum, viruses, and bacteria. Reliable purification of RNA from different sample sizes and sources TRIzol reagent performs well when processing small amounts of tissue (50–100 mg) and cells (5 × 10^6), as well as large amounts of tissue (≥1 g) and cells (>10^7). The provided protocols can be used for purifying samples from human, animal, plant, or bacterial sources. TRIzol reagent maintains RNA integrity due to its efficient inhibition of ribonuclease activity while disrupting cells and lysing cellular components during sample homogenization. The ease of use of TRIzol reagent allows for the simultaneous processing of multiple samples, with the entire process taking less than one hour. Total RNA isolated using TRIzol reagent is free from protein and DNA contamination. Formulation allows for multiple separations of various molecular targets TRIzol reagent enables the sequential precipitation of RNA, DNA, and protein from a single sample. After homogenizing the sample with TRIzol reagent, chloroform is added to separate the homogenate into a clear upper aqueous phase (containing RNA), an interphase, and a red lower organic phase (containing DNA and protein). RNA is precipitated from the aqueous phase using isopropanol, while DNA is precipitated from the interphase/organic phase using ethanol. Protein is precipitated from the phenol-ethanol supernatant using isopropanol. The precipitated RNA, DNA, or protein is washed to remove impurities and then resuspended for downstream applications.

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• 1 year ago - submitted by Yujing Zhong

Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for various cell culture applications, including washing cells before dissociation, transporting cell and tissue samples, diluting cells during counting, and preparing reagents. Formulations that contain calcium and magnesium are generally used as transport media or for reagent preparation.

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• 1 year ago - submitted by Yujing Zhong

D-Luciferin is a commonly used substrate for luciferase, widely utilized across the biotechnology field, particularly in in vivo imaging techniques. Its mechanism of action involves the oxidation of luciferin (the substrate) to produce light in the presence of ATP and luciferase. When luciferin is in excess, the number of emitted photons is positively correlated with the concentration of luciferase. After transfecting a plasmid encoding the luciferase gene (Luc) into cells and introducing it into research animals such as mice and rats, luciferin is injected, and bioluminescence imaging (BLI) is used to detect changes in light intensity. This allows for real-time monitoring of disease progression or the therapeutic effects of drugs. The influence of ATP on this reaction system can also be utilized, where changes in bioluminescence intensity indicate energy levels or vital signs.

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• 1 year ago - submitted by Yujing Zhong

Bupivacaine is a NMDA receptor inhibitor. Bupivacaine can block sodium, L-calcium, and potassium channels.Bupivacaine potently blocks SCN5A channels with the IC50 of 69.5 μM. Bupivacaine can be used for the research of chronic pain.

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• 1 year ago - submitted by Yujing Zhong

Basement membrane is a layer of matrix membrane located at the basal surface of epithelial cells in animals. OrganoGel is a matrix gel formed from basement membrane components extracted from mouse tumor tissue. The main components of this matrix gel include laminin, collagen IV, and heparan sulfate proteoglycans (Kleinman et al. 1986). Additionally, this matrix gel contains various growth factors, such as epidermal growth factor (EGF), platelet-derived growth factor (PDGF), nerve growth factor (NGF), basic fibroblast growth factor (FGF-2), transforming growth factor beta (TGF-beta), and insulin-like growth factor (IGF) (Vukicevic et al. 1992). Product Characteristics: This product is in liquid form at 4°C but becomes gel-like when heated to 37°C. Once the matrix gel has solidified, it can be returned to 4°C overnight, and the matrix gel can be re-liquefied.

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• 1 year ago - submitted by Yujing Zhong

Carprofen is a nonsteroid anti-inflammatory agent, acts as a multi-target FAAH/COX inhibitor, with IC50s of 3.9 μM, 22.3 μM and 78.6 μM for COX-2, COX-1 and FAAH, respectively.

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• 1 year ago - submitted by Yujing Zhong

Carprofen is a nonsteroid anti-inflammatory agent, acts as a multi-target FAAH/COX inhibitor, with IC50s of 3.9 μM, 22.3 μM and 78.6 μM for COX-2, COX-1 and FAAH, respectively.

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• 1 year ago - submitted by Yujing Zhong

Firefly luciferase is a protein with a molecular weight of approximately 61 kDa that catalyzes the oxidation of luciferin to oxyluciferin in the presence of ATP, magnesium ions, and oxygen, emitting bioluminescence at a wavelength of around 560 nm during the oxidation process. Renilla luciferase is a protein with a molecular weight of approximately 36 kDa that can catalyze the oxidation of coelenterazine to coelenteramide in the presence of oxygen, emitting bioluminescence at a wavelength of around 480 nm during the oxidation process. Both types of bioluminescence can be measured using a chemiluminescence instrument. Typically, the 5' UTR or promoter of the target gene is cloned upstream of the Firefly Luciferase, or the 3' UTR is cloned downstream of the Firefly Luciferase. The amount of firefly luciferase detected can be used to assess the transcriptional regulatory effects of the promoter or regulatory elements. Renilla luciferase serves as an internal control to eliminate variations in cell number, transfection efficiency, and other factors. The Dual Luciferase Reporter Gene Assay Kit first measures the activity of the firefly luciferase reporter gene using luciferin as the substrate, and then measures the activity of the Renilla luciferase reporter gene using coelenterazine as the substrate while quenching the firefly luciferase reaction. This kit is characterized by its high sensitivity.

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• 1 year ago - submitted by Yujing Zhong

DAPI is a fluorescent dye commonly used in molecular biology and cell biology research. DAPI is known for its ability to bind to DNA, specifically to the minor groove of double-stranded DNA. This dye emits blue fluorescence when excited by ultraviolet light, allowing researchers to visualize and stain DNA in various biological samples. Hoechst dyes are DNA-specific fluorescent stains that bind to the minor groove of double-stranded DNA. They emit blue fluorescence when excited by ultraviolet light. These dyes are widely used for staining DNA in various biological samples. The FluoroPure™-grade of Hoechst and DAPI powders are manufactured using stringent purification processes to minimize impurities that could interfere with fluorescence imaging. This ensures that the DAPI and Hoechst provides reliable and consistent results with minimal background noise. The >98% purity of FluoroPure grade reagents make them particularly suitable for demanding applications such as super-resolution microscopy and live-cell imaging, where precise and accurate visualization of DNA is essential.

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• 1 year ago - submitted by Yujing Zhong

Detergent, equivalent to Nonidet P-40.Triton™ X-100 is used as a non-ionic detergent, emulsifier and dispersing agent. It is useful for isolation of membrane proteins. It is also used in electrophoresis. It is an active ingredient in influenza vaccine (Fluzone). It finds applications in the dispersion of carbon materials for soft composite materials and restricting colony expansion in Aspergillus nidulans in microbiology. Further, it is an active ingredient in homemade vinyl record cleaning fluids together with distilled water and isopropyl alcohol. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

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• 1 year ago - submitted by Yujing Zhong

triple-label multiplex immunofluorescence kit，YOBIBIO Biosciences, #YB006

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• 1 year ago - submitted by Yujing Zhong

This ImmPRESS Duet Double Staining Polymer Kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section. Utilizing our unique ImmPRESS micropolymer chemistry, we independently conjugate highly-active horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme polymers to anti-mouse IgG and anti-rabbit IgG affinity-purified, highly cross-adsorbed secondary antibodies.

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• 1 year ago - submitted by Yujing Zhong

enhanced chemiluminescence detection kit，Tiangen Biotech, #PA112

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• 1 year ago - submitted by Yujing Zhong

enhanced chemiluminescence detection kit, Tiangen Biotech, #PA112

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• 1 year ago - by Anonymous

The Pierce Dilution-Free Rapid Gold BCA Protein Assay Kit is an effortless, fast, and accurate assay optimized to determine total protein concentration. It is detergent-compatible and has a 5X wider dynamic range than traditional protein assays of 0.02–10 mg/mL. This wide dynamic range eliminates the need to dilute most samples to get them into range of the assay.

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• 1 year ago - submitted by Yujing Zhong

Halt™ Protease Inhibitor Cocktail (100X), #87786

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• 1 year ago - submitted by Yujing Zhong

Taq Pro Universal SYBR qPCR Master Mix

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• 1 year ago - submitted by Yujing Zhong