Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTA3 (TTHERM_00143660)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: CET of CU428 x B2086 with plamid XL202, HTA2 with neo2 cassette replacing coding
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02878 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (btu1-? [∆2,3,4] ; BTU2/btu2-?[∆::neo1], ?)
Affected Genes: BTU (TTHERM_00348510)1, BTU2 (TTHERM_00836580)
Genomic Alteration: Macronucleus: BTU1 has the REI removed from the MREI at start of protien, BTU2 partically replaced by btu2::neo1
Availability:
References:
Synonyms:
Notes: Original strain B6-D(VB1 cells with BTU1,MREI has REI deleted but rest of BTU1 is WT, the VB1 mutation L350 to M350 is back to L350 so no longer hyper sensitive to taxol) This cell was somatically transformed. Need to check if all BTU1 is replaced
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02482 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht2[3’neo2, ∆::(HHT3,GFPc)]/hht2[3’neo2, ∆::(HHT3,GFPc)] (?HHT2/hht2[3’neo2 ∆::(HHT3,GFPc)], ?)
Affected Genes: HTT3 (TTHERM_00016170), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: C-terminal GFP- tag of HHT3, into HHT2 locus
Availability:
References:
Synonyms:
Notes: germline H3.3 +GFP at H3.2, heterokaryon. lnon-star sideof H3.3+GFP germline replacement at H3.2 locus.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01953 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; Del 4 (MPR1; mp-s, VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00987 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 ko of HHT1 and HHT2/HHF2
Availability:
References:
Synonyms:
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series All the 10.* series are separate cell lines.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01957 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (MTT1/mtt1-?[5’neo4,∆,HAHAn(::TCD2)]; pm-r, II)
Affected Genes: HPL7 (TTHERM_00551070)
Genomic Alteration: Macronucleus: Two HA tags after ATG of TCD2, neo4 in 5’ flank, inserted into MTT1 locus for over expression, need Cd
Availability:
References:
Synonyms:
Notes: HA2-TCD2 overexpession from MTT1 locus, neo 4 upstream. HA-Tcd2p localizes to micronuclei in Growing and conjugation stage.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02495 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: MPR1/mpr1-1 (MPR1; cy-s, mp-s, tr, VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00075 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (tcd3-tcd4-1[∆?::neo4]; pm-r, II)
Affected Genes: HPL4 (TTHERM_00585180), HPL5 (TTHERM_00585190)
Genomic Alteration: Macronucleus: Used one neo4 construct to KO both adjacent TCD3 and TCD4
Availability:
References:
Synonyms:
Notes: TCD3 and TCD4 gene were knock out from Mac with one construct. cell growing and development is normal.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02532 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 3 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00993 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaI-I/cdaI-1 (cdaI-1, II)
Affected Genes: CDAI (cell division arrest I)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Temperature-sensitive expression; fully penetrant at 39 degrees with high expressivity. Allelic to cdaI-2 (IA251) and cdaI-3 (IA282). Unequal fission-arrest following an anterior sliding of the oral primordium.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01449 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: exoB2/exoB2; chx1-1/CHX1-1 (exoB2; CHX1; exo-; cy-s, ?)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Submitted by Eileen Hamilton, University of California at Santa Barbara
Proper citation: RRID:TSC_SD01723 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 1R (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Deletion on right arm of chromosome 1.
Proper citation: RRID:TSC_SD00755 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaD-1/cdaD-1 (cdaD-1; cdaD, V)
Affected Genes: CDAD (cell division arrest D). Originally reported as mo6.
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: cdaD-1 causes temperature sensitive cell division arrest. Allelic with cdaD-2 (IA134). Located on chromosome 5. A detailed phenotypic analysis was carried out on a homozygous clone of a preceding sexual generation, which is now extinct.
Proper citation: RRID:TSC_SD00639 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 2L (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Deletion on left arm of chromosome 2.
Proper citation: RRID:TSC_SD00919 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htax[∆::neo2]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2 (HTAX/htax[∆::neo2]; hta1[∆::neo2]; pm-r, mp-r, ?)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration: Micronucleus: KO of HTAX and HTA1 with neo2 Macronucleus: Complete KO of HTA1 with neo2, Partial replacement of HTAX with neo2
Availability:
References:
Synonyms:
Notes: New batch, rescue of h∆∆HTA-A1F14A1 and B1G6A These are KO of the major HTA genes HTAX and HTA1 in the mic with HTAX partial in the mac. Not mating type II,V1I or VII;same as HTA1-B1a.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02388 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (?)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Collected by Paul Doerder on 5/21/2011 from the pond designated CRWP in PA (latitude 41.42, longitude -78.86)
Has a micronucleus. No mating, immature.
Proper citation: RRID:TSC_SD03112 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/hhf2,hht2[∆,::neo2] (Neo 2 KO of HHT1, WT HHT2/HHF2; pm-r, ?)
Affected Genes: HHT1 (TTHERM_00570560), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo 2 KO of HHT1 and of HHT2/HHF2 Macronucleus: Neo 2 Ko of HHT1, WT HHT2/HHF2
Availability:
References:
Synonyms:
Notes: Mate Yifan Liu’s ∆H3 2-6 x ∆H3 10-6 homozygous heterokaryons (∆HHT1 with neo, and ∆HHT2/HHF2 with neo in mic) assorted to Paromomycin sensitive in mac. Shoot with Yifan’s wildtype HHT2/HHF2 construct.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02544 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: janA-2/janA-2; eja1-1/eja1-1 (janA-2; eja1-1; janA, eja1, V)
Affected Genes: JANA (janus A) (originally reported as jan); EJA1 (enhancer of janus)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: A mutation that causes the dorsal surface to be converted to a partial mirror-image of the ventral surface and blocks conjugation. Allelic to janA-1.
Proper citation: RRID:TSC_SD00644 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[R40K,E445A, E446A,E447A]; pm-r, mp-?
, ?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with R40K, E445A, E446A, E447A and neo replacing coding of some ATU1 genes
Availability:
References:
Synonyms:
Notes: It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pR40AAA derived from pTUB100E3-PvuII (linearized)where the ATU1 gene is marked with Hae III and Pvu II sites and R40K , E445A,E446A,E447A.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02829 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht2[3’neo2,GFPc]/hht2[3’neo2,GFPc] (hht2[3’neo2,GFPc]; pm-r, ?)
Affected Genes: HHT1 (TTHERM_00189180)
Genomic Alteration: Micronucleus: GFP tagged HHT2 Macronucleus: GFP tagged HHT2
Availability:
References:
Synonyms:
Notes: Round II progeny of crossing H3.2-GFP-3x-10 (box 72:7 and 6) heterokaryons. Selected by pm and homokaryon was obtained.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02791 Copy
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