Searching the RRID Resource Information Network
Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; VPS13A/VPS13A (MPR1; VPS13A/vps13a-2::gfp; pm-r, mp-s, VII, VII)
Affected Genes: Vacuolar Protein Sorting 13A gene
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Deposited line has been grown with high concentration of paromomycin (1 mg/ml) for phenotypic assortment, but it has not been subcloned. Gene has also been referred to as TtVPS13A. Stock has been maintained in SSP medium + 2 ug/ml CdCl2 + 1 mg/ml paromomycin.
Submitted by Lawrence Klobutcher, University of Connecticut Health Center
Proper citation: RRID:TSC_SD01757 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: psmD1/psmD-1 (psmD-1, III)
Affected Genes: PSMD (pseudomacrostome D)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Pleiotropic: elongated phenotype with development of large oral-replacement or misplaced cell division oral primordia, especially in rich medium, at 39o C. Also frequent abnormal internal organization of oral structures, and fission arrest. The only known mutant allele of this gene, located on chromosome 3R. This is the most highly pleiotropic of the PSM mutant genes.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01517 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (ema1[∆?::neo3]; pm-r, VI)
Affected Genes: EMA1 (TTHERM_00088150)
Genomic Alteration: Macronucleus: Neo3 of EMA1
Availability:
References:
Synonyms:
Notes: Somatic KO of .DRH26, neo3, CU427 background
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02604 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; (mp-r, VII)
Affected Genes: HHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes
Availability:
References:
Synonyms:
Notes: Germline heteokaryon Major H3 and H4 genes knocked out with neo cassette.It mates with gl∆H3/H4 #4 strain.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01362 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ? (Partial replacement CDC2/cdc2-? CDC2 gene 5’ flanking sequence of 0 bp with GFP coding replacing CDC2 coding. Also neo3 in 3’ flanking.; pm-r with Cd+2, ?)
Affected Genes: CDC2 (TTHERM_01207660)
Genomic Alteration: Macronucleus: CDC2 gene has the 1.6 kb just upstream of ATG lacking and GFP coding replacing CDC2 coding. Also neo3 in 3’ flanking. region.
Availability:
References:
Synonyms:
Notes: XS72 Somatic transformant obtained by shooting CU428 X B2086 exconjugant cells. In CDC2 locus, coding region replaced by GFP coding. Shortened CDC2 5’ flanking region is 0 bases but the CDC2 far 5’ flanking 1 kb sequence that is 1.6 b upstream of the ATG was used to do the homologous recombination. Neo3 cassette is in 3’ flanking region.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02573 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wild type C3 (wild type C3; wild type C3, VI)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: See Figure 1 on page 13 of Methods in Cell Biology Volume 62, Tetrahymena thermophila 2000 (Editors D.J. Asai and J.D. Forney) for and excellent diagram explaining how the inbred strains are related to each other.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD00034 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Del 5 (CHX1; cy-s, VI)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01127 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (?HHT1/hht1[∆::neo2; hht2[3’neo2, S10A]; Somatic ∆HHT1 with neo cassette and HHT2S10A (neo in 3 flank) cotransformant., VI)
Affected Genes: HHT1 (TTHERM_00570560), HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: KO of HHT1, HHT2 has mutation S10A
Availability:
References:
Synonyms:
Notes: Single cell line from H3E transformant.
By antiphos H3 antibody staining, no stain so should be all HHT2 S10A. Also checked by Southern.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01886 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1];hht2[GFPc]/hht2[GFPc] (HHT2-GFP, ∆HHT3, ∆HHT4; pm-r,bsr-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: Neo KO of HHT3, Bsr KO of HHT4, GFP tagged HHT2 Macronucleus: Neo KO of HHT3, Bsr KO of HHT4, GFP tagged HHT2
Availability:
References:
Synonyms:
Notes: H3.2-GFP heterokaryon is crossed with ∆3N4B heterokaryon, the heterozygotes then go through Round I and the * side and non* side are then mated to obtain the homozygous homokaryons.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02614 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cen1-1[∆::NEO2] (cen1-1[∆::NEO2]::CEN1; WT, ?)
Affected Genes: CEN1 (TTHERM_00384910)
Genomic Alteration: Micronucleus: cen1-1[∆::NEO2] Macronucleus: cen1-1[∆::NEO2]::CEN1
Availability:
References:
Synonyms:
Notes: WT rescue of cen1∆
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03272 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/GAL1-1; chx1-1/CHX1-1 (gal1-1; chx1-1; gal-r; cy-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Generated by crossing SB1969 and SB210E. Progeny were selected as being cy-r and gal-r.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01650 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wild type D (wild type D; wild type D, V)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: See Figure 1 on page 13 of Methods in Cell Biology Volume 62, Tetrahymena thermophila 2000 (Editors D.J. Asai and J.D. Forney) for and excellent diagram explaining how the inbred strains are related to each other.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01498 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; PMR1[C3]/PMR1[C3]; MAT3/MAT3 (CHX1[C3]; PMR1[C3]; MAT3; cy-s pm-s, ?)
Affected Genes:
Genomic Alteration:
Availability:
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Synonyms:
Notes: Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01258 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Del 2L (CHX1; cy-s, VI)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Deletion on left arm of chromosome 2.
Proper citation: RRID:TSC_SD00967 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: RDR1/rdr1[∆::neo2] (?)
Affected Genes: RDR1 (TTHERM_00853150)
Genomic Alteration: Micronucleus: neo2 into RDR1
Availability:
References:
Synonyms:
Notes: Cross RDP1del X B2086. No other information
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02510 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MPR1; mp-s, VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: 6-methylpurine resistance functional heterokaryon. Regenerated micronucleus from CU428.1 by "star" mating. Non-star side maintained.
Proper citation: RRID:TSC_SD00178 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (?)
Affected Genes:
Genomic Alteration:
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Synonyms:
Notes: Collected by Paul Doerder on 8/21/2002 from the pond designated CRWP in Ridgway, PA (lat. 41 25.052, long. -78 51.876, elevation 1613 ft). Used Geko GPS.
Geographical area: Allegheny National Forest
Collection number: 453
No micronucleus present.
Proper citation: RRID:TSC_SD01546 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTA3 (TTHERM_00143660)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: CET of CU428 x B2086 with plamid XL202, HTA2 with neo2 cassette replacing coding
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02879 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[A1G] ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with A1G
Availability:
References:
Synonyms:
Notes: ∆∆HTB knockout heterokaryons rescued by htb1-A1G.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02084 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: blt1-1[∆,neo3]/ blt1-1[∆,neo3]; MPR1-?/ MPR1-? (BLT1; mpr1-1; mp-r, ?)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Micronucleus: Neo3 replaces BLT coding
Availability:
References:
Synonyms:
Notes: BLT germline heterokaryon, homozyous KO in mic, pm sensitive (120 ug/ml). Cd induced Neo cassette (1.2 ug/ml)--MTT promoter, Neo coding, BTU2 terminator. Construct pBLTMKO, Mates with BLTGL5-1 and 5-2
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02249 Copy
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