Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/gal1-1 (GAL1; gal-s, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01665 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E438A]; pm-r, mp-?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E438A or neo replacing coding of some ATU1 genes
Availability:
References:
Synonyms:
Notes: It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pTUB100E3-PvuII (linearized). Its mac ATU1 gene is marked with Hae III and Pvu II sites and has E438A
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01828 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; pmr1-1/pmr1-1; MAT3/MAT3 (CHX1[C3];pmr1-1; MAT3; cy-s, pm-r, ?)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01278 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (MTT1/mtt1[5neo2, ∆,(::HTAY1-122)]; pm-r, ?)
Affected Genes: HTAY (TTHERM_01079200)
Genomic Alteration: Macronucleus: Partial replacement of MTT1 coding by N-terminus of HTAY, aa 1-122, with neo2 in 5’ flank of MTT1
Availability:
References:
Synonyms:
Notes: Somatic transfomation of CU428xB2086 with pXS87. (MTT-neo2-2HA-HTAHTAY codes for histone variant protein H2A.Y into MTT1 locus, Incomplete replacement.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02881 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; Nulli 2L,5 (MPR1; mp-s, IV)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00100 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 5 [B or C3] (cnj3, pm-r, VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00356 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-? [E432D,E435D]; pm-r, BTUI has(E432,435D), BTU2 is replaced by bsr1)
Affected Genes: BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: knockout of btu1 with neo
knockout of btu2 with bsr Macronucleus: BTU1 has only (E432, 435D) no WT, BTU2 is KO
Availability:
References:
Synonyms:
Notes: Progeny of DB6B x DB2A, transformed by BTU1-1235D mutation construct (E432,435D)
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01843 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Collected by Paul Doerder on 7/25/2009 from the pond designated Perkins Pond in NH (latitude 42.84, longitude -72.14)
Has a micronucleus.
Proper citation: RRID:TSC_SD03111 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: drh1[∆?::neo4]/drh1[∆?::neo4]
Affected Genes: DRH1 (TTHERM_00190830)
Genomic Alteration: Micronucleus: Neo4 into 5’ end of coding of DRH1
Availability:
References:
Synonyms:
Notes: Neo4 KO cassette in DRH1 locus, homozygous KO in mic, mac assorted to pm-s. Two cell lines in row. Mate with A5, H9, and C10 cell lines. Homozygous heterokaryon.
Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryon KOs do not grow well.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02141 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hho1[3’neo2, T35E, S43E, S45E, T47E, T54E, GFPc]; pm-r, VII)
Affected Genes: HHO1 (TTHERM_00823720)
Genomic Alteration: Macronucleus: Change Threonine 35, 47, 54 and serine 43, 45 to glutamic acid
Availability:
References:
Synonyms:
Notes: Complete somatic tranformant of CU428 cells with pHHOE5-GFP /neo construct. Phosphorylation sites 1- 5 of H1 changed to glutamic adid, neo cassette in 3’ flanking region for selection.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02301 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 5 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01179 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-1[K350M]/ btu1-1[K350M] (BTU1 locus replaced by far 5’BTU1 5’--neo3--CDC2 5’ flanking 1.6kb immediately upstream of ATG--GFP coding--BTU1 3’; pm-r with Cd+2; tax-r, ?)
Affected Genes: CDC2 (TTHERM_01207660)
Genomic Alteration: Macronucleus: BTU1 locus replaced by far 5’BTU1 5’--neo3--CDC2 5’ flanking 1.5 kb immediately upstream of ATG--GFP coding--BTU1 3’
Availability:
References:
Synonyms:
Notes: Complete somatic transformant of CU522 cells. Construct was BTU1 5’(600bp from400-1000bp upstream of BTU1 ATG)--Neo3--CDC2 5’flanking 1600 bp immediately upstream of ATG--GFP coding- -1.4 Kb BTU1 3â€Â. BTU1 promoter was deleted.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02822 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ubc9-1[Δ::neo3]/ubc9-1[Δ::neo3]; pmr1-1/pmr1-1; mpr1-1/mpr1-1 (UBC9; PMR1; MPR1; pm-s;mp-s, VII)
Affected Genes:
Genomic Alteration: Micronucleus: ubc9-1[Δ::neo3]/ubc9-1[Δ::neo3]
Availability:
References:
Synonyms:
Notes: Submitted by James Forney, Purdue University
Proper citation: RRID:TSC_SD01737 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: pmr1-1/pmr1-1; chx1-1/chx1-1 (PMR1; CHX1; pm-s, cy-s, VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01615 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; Nulli 4 (MPR1; mp-s, VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00889 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Del 2L (CHX1; cy-s, VI)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Large deletion. May be missing entire left arm of chromosome 2.
Proper citation: RRID:TSC_SD00808 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 5 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01181 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 5 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01186 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 5 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01185 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/GAL1-1; chx1-1/CHX1-1 (gal1-1; chx1-1; gal-r; cy-r, VI)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Generated by crossing SB1969 and SB210E. Progeny were selected as being cy-r and gal-r.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01584 Copy
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