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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
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    • RRID:TSC_SD00715

    This resource has 10+ mentions.

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (CHX1; cy-s, VI)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Cycloheximide resistance functional heterokaryon.Regenerated micronucleus from CU427.2 by "star" mating. Non-star side maintained.

Proper citation: RRID:TSC_SD00715 Copy   

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi11[∆?::neo3]/twi111[∆?::neo3] (twi11[∆?::neo3]/twi111[∆?::neo3]; MTT1/mtt1-?[5’ bsr1,∆,(::CNA1), GFPc]; bsr-r, pm-r, GFP tagged Cna1p, ?)
Affected Genes: CNA1 (TTHERM_00146340), TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: TWI11replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
Availability:
References:
Synonyms:
Notes: ∆TWI11 homokaryon transformed with Bsr-MTT1-CNA1-GFP. CNA1-GFP seq. was used to replace MTT1 coding seq. Bsr1 cassette was inserted at the 5’F seq. of MTT1 locus. The construct was transformed into mac. Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

Proper citation: RRID:TSC_SD01809 Copy   

    • RRID:TSC_SD02580

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; (Chimeric H3 with GFP tagged H3 chimera protein 4, H3(1-79) H3.3(80-135); pm-r, ?)
Affected Genes: HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170), HHT1 (TTHERM_00570560), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes Macronucleus: GFP tagged chimeric H3
Availability:
References:
Synonyms:
Notes: Germline heteokaryon Major H3 and H4 genes knocked out with neo cassette.It GFP tagged H3 chimera protein 4, H3(1-79) H3.3(80-135), rescue the progeny of major H3 H4 KO heterokaryons. can be mated with B2086. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

Proper citation: RRID:TSC_SD02580 Copy   

    • RRID:TSC_SD01014

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Nulli 2 (MPR1; mp-s, VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:

Proper citation: RRID:TSC_SD01014 Copy   

    • RRID:TSC_SD02068

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (TWI1::HA-TWI1-neo3, VII)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: CU428 was transformed with HA-TWI1-neo3 and endogenous TWI1 loci were completely replaced. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

Proper citation: RRID:TSC_SD02068 Copy   

    • RRID:TSC_SD01413

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (GTU1/gtu1[3’neo2neo2,HAc], VII)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Macronucleus: HA tag at c-terminus of GTU1 with neo2 in 3’ flanking region
Availability:
References:
Synonyms:
Notes: GTU (gamma tubulin) gene with c-terminal HA tag and two neo2 cassette inserted in 3’ flanking region. Partial replacement From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

Proper citation: RRID:TSC_SD01413 Copy   

    • RRID:TSC_SD02509

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: RDR1/rdr1[∆::neo2] (?)
Affected Genes: RDR1 (TTHERM_00853150)
Genomic Alteration: Micronucleus: neo2 into RDR1
Availability:
References:
Synonyms:
Notes: Cross RDP1del X B2086. No other information From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

Proper citation: RRID:TSC_SD02509 Copy   

    • RRID:TSC_SD00170

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; gal1-1/gal1-1; Nulli 2,3,4,5 (CHX1[A]; GAL1[A]; MPR1[A]; cy-s, gal-s, mp-s, III)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:

Proper citation: RRID:TSC_SD00170 Copy   

    • RRID:TSC_SD02197

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (HA tagged HTAY and V5 tagged PPO1; cy-r, pm-r, VII)
Affected Genes: PPO1 (TTHERM_00865290)
Genomic Alteration: Macronucleus: HA tagged HTAY and PPO1 with n-terminal V5 tag
Availability:
References:
Synonyms:
Notes: HA2-HTAY cells transformed with V5-PP50 with the cycloheximide resistance cassette H4-rpL29-B upstream. Partial replacement . Cells are very sick, give yellow-green auto fluorescence with many vacuoles. Do give the correct ~36 kDa Western band for anti V5. Grow with 15 ug/ml cy and 80 ug/ml pm. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

Proper citation: RRID:TSC_SD02197 Copy   

    • RRID:TSC_SD01660

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/GAL1-1; chx1-1/CHX1-1 (gal1-1; chx1-1; gal-r; cy-r, VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Generated by crossing SB1969 and SB210E. Progeny were selected as being cy-r and gal-r. Submitted by Eileen Hamilton, University of California, Santa Barbara

Proper citation: RRID:TSC_SD01660 Copy   

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi1[∆::neo3]/twi1[∆::neo3] (?)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Micronucleus: Neo3 replaces coding of TWI1
Availability:
References:
Synonyms:
Notes: Homozygous heterokaryon of TWI1 gene with neo3, derived from B2086XCU428. Can mate with ∆TWI1hk-C4 and CU427. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

Proper citation: RRID:TSC_SD02116 Copy   

    • RRID:TSC_SD00218

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: pmr1-1/pmr1-1; cnj5-1/cnj5-1 (cnj5-1; pmr1-1; cnj5, pm-r, II)
Affected Genes: CNJ5
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:

Proper citation: RRID:TSC_SD00218 Copy   

    • RRID:TSC_SD02242

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-1[5’neo2,∆,HAc(::BTU11-428,BLT1428-473))], VII)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Macronucleus: BTU1(AA 1-428) with BLT at C terminus (AA 428-473) followed by HA tag, inserted into the MTT1 locus. Neo2 upstreamin MTT1 5’ region
Availability:
References:
Synonyms:
Notes: CU428 with BLT/BTU1 chimeric gene in MTT1 locus with HA tag. CU428 somatic transformant with pbBc-1 construct. BTU1(AA 1-428) with BLT at Cterminus (AA 428-473) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

Proper citation: RRID:TSC_SD02242 Copy   

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (btu1-? [∆2,3,4] ; BTU2/btu2-?[∆::neo1], ?)
Affected Genes: BTU (TTHERM_00348510)1, BTU2 (TTHERM_00836580)
Genomic Alteration: Macronucleus: BTU1 has the REI removed from the MREI at start of protien, BTU2 partically replaced by btu2::neo1
Availability:
References:
Synonyms:
Notes: Original strain B6-D(VB1 cells with BTU1,MREI has REI deleted but rest of BTU1 is WT, the VB1 mutation L350 to M350 is back to L350 so no longer hyper sensitive to taxol) This cell was somatically transformed. Need to check if all BTU1 is replaced. From the Gorovsky lab, University of Rochester.

Proper citation: RRID:TSC_SD02481 Copy   

    • RRID:TSC_SD03296

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: sfr13-1[∆::NEO2] (sfr13-1[∆::NEO2]; Reduced cell size, defective oral apparatus, fewer basal bodies, basal body stability defect, basal body separation and reorientation defect., ?)
Affected Genes: SFR13 (TTHERM_00522830)
Genomic Alteration: Micronucleus: cen2-1[∆::NEO2]
Availability:
References:
Synonyms:
Notes: sfr13 complete null. From the Winey Lab at the University of Colorado Boulder.

Proper citation: RRID:TSC_SD03296 Copy   

    • RRID:TSC_SD02001

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ∆BTU1/∆BTU1, ∆BTU2/∆BTU2, chx+/chx+,Mpr?/Mpr? (mp-r)
Affected Genes: BTU1 (TTHERM_00348510) + BTU2 (TTHERM_00836580)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: BTU1,BTU2 double germline knockout heterokaryon originally from Jacek Gaertig. ∆BTU1 by neo cassette, ∆BTU2 by blasticidin(BSR) cassette. It can mate with DB6B, received from Jacek Gaertig, 3/8/00. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

Proper citation: RRID:TSC_SD02001 Copy   

    • RRID:TSC_SD01398

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta2[∆::neo2]/hta2[∆::neo2]; mpr-1/mpr-? (mp-r, ?)
Affected Genes: HTA2 ( TTHERM_00316500 )
Genomic Alteration: Micronucleus: Neo 2 into HTA1, replaces coding
Availability:
References:
Synonyms:
Notes: CU428xB2086 HTA2 germline knockout heterokaryon transform with pQR17, remove all coding by neo. Can mate with G209A1 and G209C4. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

Proper citation: RRID:TSC_SD01398 Copy   

    • RRID:TSC_SD01950

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[5’neo2,GFPc]/hht3[5’neo2,GFPc] (?)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Micronucleus: GFP tagged HHT3
Availability:
References:
Synonyms:
Notes: non-star side of H3.3+GFP germline replacement heterokaryon. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

Proper citation: RRID:TSC_SD01950 Copy   

    • RRID:TSC_SD02801

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi11[5’neo3,HAn]/twi11[5’neo3,HAn] (twi11[5’neo3,HAn]; pm-r, ?)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: TWI11-HA with neo3 in 5’ region Macronucleus: TWI11-HA with neo3 in 5’ region
Availability:
References:
Synonyms:
Notes: Homozygous HA-tagged TWI 11 homokaryon.HA coding sequence was inserted just after the start codon of TWI11. neo3 cassette was inserted upstream of TWI11 coding. mates wtih F2-5,F2-8. From the Gorovsky lab, University of Rochester.

Proper citation: RRID:TSC_SD02801 Copy   

    • RRID:TSC_SD00629

https://sites.wustl.edu/tetrahymena/finding-strains/

Source Database: TSC, Tetrahymena Stock Center
Genetic Background: disA-1/disA-1 (disA-1; disA, VII)
Affected Genes: DISA (disorganized A)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: disA-1 is a partially ts mutation that brings about disorganization of ciliary rows, without affecting the positioning of major cortical landmarks, or cytokinesis.

Proper citation: RRID:TSC_SD00629 Copy   

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