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http://www.dfhcc.harvard.edu/core-facilities/tissue-microarray-and-imaging-pathology/

Core facility that provides the following services: Construction of standard and custom TMA service, Nucleic acid isolation from paraffin embedded tissue service, Immunofluorescent staining service, Access to the Automated Quantitative Analysis (AQUA) system, Tissue microarray consultation service.

The mission of the Tissue Microarray and Imaging Core is to generate tissue microarrays by allowing placement of up to 480 0.6 mm diameter tissue samples into a single standard tissue block, allowing hundreds of tumors and samples to be evaluated on a single slide. The DF/HCC Tissue Microarray & Imaging (TMI) Core is dedicated to the construction and evaluation of high quality TMAs for cancer research. In addition, the Core performs high-throughput isolation of DNA and RNA from formalin-fixed paraffin-embedded tissues. The Core also provides web-based digital pathology services using the Aperio system. These services include slide scanning, image viewing and storage, and quantitative image analysis, which facilitate tissue-based research. Finally, the Core provides access to the Automated Quantitative Analysis (AQUA) system.

Proper citation: DF/HCC Tissue Microarray and Imaging Core Facility (RRID:SCR_009743) Copy   

•   Source: SciCrunch Registry

http://harvard.eagle-i.net/i/0000012c-6056-ed57-c437-ff0b80000000

Core facility that provides the following services: Protein development/cloning, Western blot service, ELISA service, Flow cytometry service.

The mission of the DF/HCC Monoclonal Antibody Core (MAC) is to produce novel monoclonal antibodies that are directed against antigens of interest to Dana-Farber, the Harvard Cancer Center, and affiliated investigators. These monoclonal antibodies may be useful for basic research, drug discovery and clinical applications including diagnosis, surrogate markers for disease status, response to therapy or drug toxicity. Given the diverse research needs of investigators at DFCI and the Harvard Community, the MAC should strive to support a wide range of antibody requests. A key component of the DF/HCC MAC is the ability to explore and develop new technologies that facilitate generation of monoclonal antibodies. The DF/HCC MAC should be capable of supporting all basic functions of generating, screening, storing and producing monoclonal antibodies.

Proper citation: DF/HCC Monoclonal Antibody Core (RRID:SCR_009740) Copy   

•   Source: SciCrunch Registry

http://harvard.eagle-i.net/i/0000013e-8557-6524-8a53-38db80000000

There is a growing need for animal models to carry out in vivo developmental and regenerative medicine studies of human cells, tissues and organs. The Humanized Neonatal Mouse Center (HNMC) was created to accelerate research in the stem cell field by providing humanized mouse models to study human stem cell engraftment and differentiation in regenerative medicine. We have over 4 years of experience in constructing different types of humanized mouse models, including neonatal heart, lung and kidney injury models. We have extensive experience in hematopoietic stem cell reconstruction. It is our goal at HNMC to facilitate collaborative research in human stem cell biology, where physiologically relevant microenvironments (niches) may be created in vivo to study human stem cell fate and function under experimental settings where disease, damage or degenerative conditions can be controlled. We can provide customized humanized mouse models to the HSCI research community, to collaborate on research studies of common interest, and to advance the general use of these models for a broad range of translational and preclinical studies.

Proper citation: HSCI Humanized Neonatal Mouse Center (RRID:SCR_009815) Copy   

•   Source: SciCrunch Registry

http://harvard.eagle-i.net/i/00000141-ec2a-514f-89f9-43eb80000000

Core facility that provides the following services: Project Consultation, CADD Design, and Fabrication. We have many years of experience designing and manufacturing custom tools for medical research. This has allowed us to compile a large collection of different solutions to a wide range of problems. We work on a daily basis with researchers working in these areas of medical science: * Cell Biology * Genetics * Anesthesiology Research * Surgical Research * Pathology Research * Respiratory Biology * Environmental Sciences

Proper citation: HMS Systems Biology Quad Machine Shop (RRID:SCR_009812) Copy   

•   Source: SciCrunch Registry

http://harvard.eagle-i.net/i/0000012b-00c3-414f-db6e-7a3f80000000

Core facility that provides the following services: Protein identification from SDS-PAGE gel bands/spots (Coomassie stained level of protein), Protein phosphorylation service, TCA samples service. The Taplin Biological Mass Spectrometry Facility opened in February 2001 as a core facility for the analysis of proteins and peptides by mass spectrometry. The facility is focused on serving the needs of investigators at Harvard Medical School (HMS) and all the Harvard affiliated Institutions.

Proper citation: HMS Taplin Mass Spectrometry Core Facility (RRID:SCR_009813) Copy   

•   Source: SciCrunch Registry

https://nic.med.harvard.edu/

Core facility that provides the following services: User-based microscopy service, Live Specimen Imaging, Widefield Fluorescence, Spinning Disk Confocal Microscopy, Laser Scanning Confocal, Color Imaging, Image Analysis, Microinjection, Phase Contrast and Differential Interference Contrast (DIC), Photo-bleaching, Photo-activation, & Laser Ablation, Microfluidic Perfusion, Imaging and microscopy training. The mission of the NIC@HMS is to: enhance basic research by providing access to state-of-the-art microscopy and imaging equipment, provide training courses on basic and advanced light microscopy techniques for the benefit of HMS and the greater Boston research community, introduce the latest innovations in light microscopy and imaging to the HMS community, serve as a learning center for our corporate partners and contributions, and provide a dedicated NIC@HMS director for ongoing technical consultation and support.

Proper citation: HMS Nikon Imaging Center (RRID:SCR_009810) Copy   

•   Source: SciCrunch Registry

http://dartmouth.eagle-i.net/i/0000013f-cf91-dd1f-0b49-0d2b80000000

The Ethics Consultation Core (ECC) leverages current ethics activities across Dartmouth, and thus provides the capacity for real-time collaborative ethics advice for investigators throughout the translational research community.

Proper citation: Dartmouth SYNERGY: Ethics Consultation Core (RRID:SCR_009775) Copy   

•   Source: SciCrunch Registry

http://dartmouth.eagle-i.net/i/0000013e-6135-0e20-8e79-05bd80000000

The BCC provides essential statistical and quantitative methods support to Dartmouth investigators, helping to remove impediments to initiating new clinical translational research programs for young physicians and other scientists. Specialized support for statistical genetics/genomics, decision sciences and comparative effectiveness research, health services research, mathematical demography, psychometrics, geographic information systems, and imaging applications. The BCC also provides statistical expertise related to critical data management, in cooperation with the SYNERGY Biomedical Informatics Core, and fully supports statistical education initiatives throughout SYNERGY

Proper citation: Dartmouth SYNERGY: Biostatistics Consultation Core (RRID:SCR_009773) Copy   

•   Source: SciCrunch Registry

http://harvard.eagle-i.net/i/00000130-08de-721d-de5a-673e80000000

Core facility that provides the following services: Confocal microscopy.

Confocal microscopy offers multiple benefits compared to conventional microscopy, particularly the ability to collect serial, high-resolution optical sections from thick specimens. Multicolor, three-dimensional images can be obtained, providing high-content information from single images. To assist NERCE investigators from throughout the region, NERCE has established a Confocal Microscope Facility at the University of Massachusetts Medical School. Funding through the NERCE has procured a state-of-the-art LEICA SP2 AOBS laser scanning confocal microscope, which combines spectrophotometric detection with confocal microscopy. The instrument is located at the BL2+ facility at the Lazare Research Building, at the University of Massachusetts Medical School, Worcester, MA. The use of live cell imaging combined with green fluorescent protein (GFP) technology is an exciting methodology with which to study the interactions between infectious agents and living cells. A variety of applications are amenable to confocal microscopy techniques and include: monitoring the localization of proteins in living or fixed cells, monitoring changes in the cellular distribution of proteins, and monitoring changes in the activation status and morphological changes in cells exposed to infectious agents.

Proper citation: HMS NERCE Confocal Microscope Resource (RRID:SCR_009805) Copy   

•   Source: SciCrunch Registry

http://harvard.eagle-i.net/i/0000012c-c7b2-aa59-a061-4a6580000000

Core facility that provides the following services: Imaging system training, Live cell imaging.

Live-cell imaging is a powerful method that enables investigators to observe cellular trafficking events with a high degree of spatial and temporal resolution, including visualization of individual bacteria or viral particles engaged in the process of infecting eukaryotic cells. A quantitative description of bacterial and viral invasion mechanisms can be obtained through such approaches. NERCE has provided funding to support the use of live-cell imaging for any New England investigator studying NIAID priority pathogens and agents of emerging infectious disease. Investigators interested in working with the Live-Cell Imaging core laboratory should complete the Imaging Core Request Form and send it to Gerald Beltz. There are no fees for use of the live-cell imaging core for research related to NIAID priority pathogens and agents of emerging infectious disease. Please note that since the NERCE program will end in February 2014, our ability to support new requests for services is limited.

Proper citation: HMS NERCE Live-cell Imaging Core (RRID:SCR_009806) Copy   

•   Source: SciCrunch Registry

http://harvard.eagle-i.net/i/0000012e-01e7-f2ed-b2b9-4d8780000000

The molecular electron microscopy facility at Harvard Medical School is predominantly used for our own research and can therefore not be considered a service facility. We are, however, committed to make our technology available to as many research groups in the community as possible. If you are interested to use molecular electron microscopy, please contact us by phone or email.

Proper citation: HMS Molecular Electron Microscopy Facility (RRID:SCR_009802) Copy   

•   Source: SciCrunch Registry

http://dartmouth.eagle-i.net/i/0000012b-00c4-3214-6a59-ec4e80000000

Core facility that provides the following services: Genotyping service, DNA sequencing service, DNA fragment analysis.

DNA Sequencing, DNA Fragment Analysis

Proper citation: Dartmouth Molecular Biology Shared Resource (RRID:SCR_009766) Copy   

•   Source: SciCrunch Registry

http://dartmouth.eagle-i.net/i/00000135-59ea-7c4f-a5f8-842480000000

Core facility that provides the following services: Multi-photon imaging. This SynergyT1 Shared Resource Core includes two microscopes sharing two femtosecond pulsed Ti:Sapphire lasers (tunable between 690 and 1080 nm) for imaging fluorescently-tagged molecules in tissues in vivo and in vitro.

Proper citation: Dartmouth Multi-Photon Imaging (RRID:SCR_009767) Copy   

•   Source: SciCrunch Registry

http://harvard.eagle-i.net/i/00000142-0084-cc9f-89f9-43eb80000000

Core facility that provides the following services: Instrument operation and data analysis.

Instrumentation for this facility includes an Agilent 6520 Accurate-Mass quadrupole time-of-flight (Q-TOF) LC/MS system with great mass accuracy, mass resolution, and sensitivity for small molecule mass identification/quantitation and metabolomics experiments. It is equipped with an Agilent 1200 series HPLC with DAD UV/V is detection for normal flow LC/MS and nitrogen CID for MS/MS. The Q-TOF LC/MS is located in laboratory of Dr. Suzanne Walker in the Department of Microbiology and Immunobiology.

Proper citation: HMS Microbiology and Immunobiology Biological Chemistry Mass Spec Facility (RRID:SCR_009800) Copy   

•   Source: SciCrunch Registry

http://dartmouth.eagle-i.net/i/0000012a-24fd-03f4-d8a1-249280000000

Core facility that provides the following services: Electron micrscopy facility access.

The EM Facility is an institutional resource to meet the electron microscopy needs of faculty, staff and students of the Dartmouth College community, including the College, Thayer School of Engineering, the Dartmouth Medical School, and the Dartmouth-Hitchcock Medical Center. The instruments are housed in the EM wing of Remsen. Because the facility is intended to provide service to all the campus, yet has limited personnel, a major effort is made to provide training in the use of appropriate instruments to enable users to do their own microscopy. In addition to this training, there will be service assistance available for those unable to spend time on the microscopes, and for processing of biopsy samples from DHMC.

Proper citation: Dartmouth Electron Microscope Facility (RRID:SCR_009762) Copy   

•   Source: SciCrunch Registry

http://dms.dartmouth.edu/dgml

Core facility that provides the following services: Illumina Bead Array service, Affymetrix Microarray service.

The Genomics Shared Resource provides technologies to the Norris Cotton Cancer Center and Dartmouth community investigators that enable profiling of gene expression, miRNA, GpC Island, and CGH on a whole-genome scale. The long-term goal of the Genomics Shared Resource is to provide an efficient and affordable fee-for-service operation that will provide high quality genomics and microarray data for the growing number of Cancer Center investigators who require this service.

Proper citation: Dartmouth Genomics Shared Resource (RRID:SCR_009763) Copy   

•   Source: SciCrunch Registry

http://dartmouth.eagle-i.net/i/0000012b-43eb-535f-bd3e-55f580000000

Core facility that provides the following services: Cigarette smoke exposure analysis service.

Our lab is interested in the innate immune system at the mucosal surfaces of the female reproductive and respiratory tracts. A special focus of this work is to determine the effects of cigarette smoke on changes in immune protection. The relationship of cigarette smoke exposure to increased risk of infection is well established, however the mechanisms involved are not. Our research shows that cigarette smoke exposure reduces production of a key antimicrobial chemokine, CCL20. Ongoing research in the lab seeks to determine if similar responses to cigarette smoke exposure underlie increased rates of infection and how cigarette smoke exposure associated infection is linked to the development of cancer. Consulting; training; planning; experimental management assistance. Banked tissue samples available for preliminary studies. Small animal cigarette exposure.

Proper citation: Dartmouth Cigarette Smoke Exposure Analysis Laboratory (RRID:SCR_009761) Copy   

•   Source: SciCrunch Registry

http://harvard.eagle-i.net/i/0000012b-00c0-1e2d-db6e-7a3f80000000

Core facility that provides the following services: Complex protein mixture analysis service, Project consultation service, Data analysis service, Protein identification service, Posttranslational modification site determination service, N-terminal Edman Sequence Analysis Service, C-terminal sequence analysis service, De novo sequence analysis service, Quantitative proteomics service, Labeled quantitative proteomics - SILAC, Labeled quantitative proteomics - ICAT service, Labeled quantitative proteomics - iTRAQ service, Labeled quantitative proteomics - AQUA service, Intact molecular weight determination service, Low resolution GC/MS service, Low resolution GC/MS/MS service, Exact mass MS & MS/MS electrospray for structural elucidation service. The FAS Center for Systems Biology Mass Spectrometry and Proteomics Resource Laboratory provides mass spectrometry and strategic consulting in Proteomics and Small Molecule analysis for Life Science and Chemistry researchers as well as others worldwide. This resource brings together the state-of-the-art expertise and instrumentation of the Microchemistry and Proteomics, CCB Mass Spectrometry, and Bauer Center Core laboratories, leveraging our breadth of experience to provide the best possible support for your research.

Proper citation: Harvard FAS Bauer Core: Mass Spectrometry and Proteomics Core Laboratory (RRID:SCR_009837) Copy   

•   Source: SciCrunch Registry

http://harvard.eagle-i.net/i/00000130-756e-db1e-1971-4f2080000000

Core facility that provides the following services: Nanofabrication cleanroom and instrumentation access, instrument training, staff assistance. The Center for Nanoscale Systems'' Nanofabrication Facility (CNS-NF), offers resource and staff support for fabricating and characterizing nanoscale devices and structures. The facility currently operates the 10,000 sq.ft. LISE Cleanroom with leading-edge equipment capable of electron-beam and optical lithography, physical and chemical vapor deposition, dry and wet processing, metrology, and device characterization.

Proper citation: Harvard CNS Nanofabrication Facility (RRID:SCR_009832) Copy   

•   Source: SciCrunch Registry

http://harvard.eagle-i.net/i/00000130-7574-3d13-1971-4f2080000000

The National Nanotechnology Infrastructure Network?s computational activity (NNIN/C) is the computational counterpart of the NNIN experimental initiative. Like the NNIN experimental program, NNIN/C is a multi-university initiative, funded by the National Science Foundation (NSF). Harvard University serves as the coordinating hub of NNIN/C, the object of which is to establish a national computing resource that provides hardware resources and simulation tools dedicated to nanoscience research for the academic and industrial research communities. Strong technical and scientific support is provided by staff experts so that the tools and resources can benefit interdisciplinary research. The software tools include commercial software packages for design, characterization and analysis of nanometer scale devices as well as some of the latest academic advances in nanoscale modeling and simulation software. The goals and strategies of NNIN/C are: * Assemble and create a wide suite of robust software that addresses critical issues related to the physical, chemical, and biological properties of artificial and natural nanoscale structures. * Maintain and, where necessary, modify these simulations to address a broad range of research problems. * Provide strong technical support and thorough instruction on the software tools so that even novice users can rapidly develop solutions to their own unique research problems. * Provide web-based graphical user interfaces (GUIs) for user-friendly access to simulation tools as well as web-based resources for instruction and feedback to the community of researchers employing the same tools

Proper citation: Harvard CNS NNIN/C Computational Facility (RRID:SCR_009830) Copy   

•   Source: SciCrunch Registry