| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
pERB258 Resource Report Resource Website |
RRID:Addgene_67760 | Kif5b(560)-mCherry-eDHFR | Homo sapiens | Ampicillin | PMID:25989077 | 2021-11-24 03:37:35 | 0 | |||
|
pDONR223_PIK3R1_WT Resource Report Resource Website |
RRID:Addgene_81748 | PIK3R1 | Homo sapiens | Spectinomycin | PMID:27147599 | Open state: NO stop codon immediately follows insert | Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin | E181K | 2022-12-10 12:07:09 | 0 |
|
pDONR223-PIK3R1 Resource Report Resource Website |
RRID:Addgene_23696 | PIK3R1 | Homo sapiens | Spectinomycin | PMID:21107320 | The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately. | Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin | 2022-12-10 12:04:30 | 0 | |
|
pLenti4/V5-DEST-P85(T576del) Resource Report Resource Website |
RRID:Addgene_40228 | P85(T576del) | Homo sapiens | Ampicillin | PMID: | Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | deleted amino acid 576 | 2022-12-10 12:05:10 | 0 | |
|
pLenti4/V5-DEST-P85(N564K) Resource Report Resource Website |
RRID:Addgene_40226 | P85(N564K) | Homo sapiens | Ampicillin | PMID: | Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | changed Asparagine 564 to Lysine | 2022-12-10 12:05:10 | 0 | |
|
pLenti4/V5-DEST-P85(K550_K562dup) Resource Report Resource Website |
RRID:Addgene_40224 | P85(K550_K562dup) | Homo sapiens | Ampicillin | PMID: | Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | created internal duplication of amino acids 550-562 | 2022-12-10 12:05:10 | 0 | |
|
pFastBac1 PIK3R1-Ni Resource Report Resource Website |
RRID:Addgene_17744 | PIK3R1-Ni | Homo sapiens | Ampicillin | PMID:15950905 | Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | Partial cDNA | 2022-12-10 12:03:41 | 0 | |
|
pLenti4/V5-DEST-P85(KS459del) Resource Report Resource Website |
RRID:Addgene_40222 | P85(KS459delN) | Homo sapiens | Ampicillin | PMID: | Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | amino acids 459-460 replaced with asparagine | 2022-12-10 12:05:09 | 0 | |
|
pLenti4/V5-DEST-P85(E439del) Resource Report Resource Website |
RRID:Addgene_40221 | P85(E439del) | Homo sapiens | Ampicillin | PMID: | Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | deleted amino acid 439 | 2022-12-10 12:05:09 | 0 | |
|
pGL3-CMV-p85a 3'UTR mutant Resource Report Resource Website |
RRID:Addgene_51393 | p85alpha 3'UTR | Homo sapiens | Ampicillin | PMID:19079265 | miR-29 seed mutant (TGCT->GTAG) | Backbone Marker:Promega (modified to have 3' multi cloning sites); Vector Backbone:pGL3-UC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | miR-29 binding site mutant | 2022-12-10 12:05:40 | 0 |
|
pcDNA3.1_iSH2-pMagFast2(3x)-iRFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_67298 | iSH2-pMagFast2(3x)-iRFP | Homo sapiens | Ampicillin | PMID:25708714 | Backbone Marker:Invitrogen; Backbone Size:5354; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-12-10 12:06:25 | 1 | ||
|
pBSSK p85 alpha (delta BH) Resource Report Resource Website |
RRID:Addgene_13431 | p85 alpha (delta BH) | Homo sapiens | Ampicillin | PMID:16880400 | see author's map for more information. | Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | BH domain replaced by flag tag. | 2022-12-10 12:01:56 | 0 |
|
pGEX p85a(N)-SH2 Resource Report Resource Website |
RRID:Addgene_46464 | p85a(N) | Homo sapiens | Ampicillin | PMID:17588523 | The molecular weight of the GST fusion protein is approximately 38.5 kDa | Backbone Marker:GE Healthcare Biosciences; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | contains amino acids 321-434 | 2022-12-10 12:05:26 | 0 |
|
pGEX p85a(C)-SH2 Resource Report Resource Website |
RRID:Addgene_46463 | p85a(C) | Homo sapiens | Ampicillin | PMID:17588523 | The molecular weight of the GST fusion protein is approximately 38.4 kDa | Backbone Marker:GE Healthcare Biosciences; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | contains amino acids 612-724 | 2022-12-10 12:05:26 | 0 |
|
pCDNA3-EMTB-TurboID-V5 Resource Report Resource Website |
RRID:Addgene_190736 | ensconsin | Homo sapiens | Ampicillin | PMID:35952650 | Backbone Size:5403; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Microtubule binding-domain of ensconsin (amino acids 18-282 of GenBank reference sequence X73882) | 2022-12-10 12:04:13 | 0 | |
|
pcDNA3-mCherry-Rheb(C181S) Resource Report Resource Website |
RRID:Addgene_192437 | mCherry-Rheb(C181S) | Homo sapiens | Ampicillin | PMID:35294906 | Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Rheb Cys 181 mutated to Ser; abolishes C-terminal farnesylation. | 2022-12-09 12:04:16 | 0 | |
|
pcDNA3-mCherry-TSC2(AA,3Q)-NLS Resource Report Resource Website |
RRID:Addgene_192445 | mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS | Homo sapiens | Ampicillin | PMID:35294906 | Backbone Size:5472; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in isoform 1) mutated to Ala; Lys 1614, Lys 1615, and Arg 1616 mutated to Gln. | 2022-12-09 12:04:17 | 0 | |
|
pSCM167-Venus-VF-hADAM10(aa220-459) Resource Report Resource Website |
RRID:Addgene_189555 | truncated ADAM metallopeptidase domain 10 | Homo sapiens | Ampicillin | PMID: | Please note: Plasmid contains a Y316H mutation in hADAM10 compared to the NCBI reference sequence NP_001101.1. This mutation was present in the original DNA that was was PCR amplified from human placenta cDNA and is not known to affect plasmid function. | Backbone Marker:Customized backbone from Fu lab ; Vector Backbone:pSCM167-Venus (Fu lab); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | contains aa 220-459 | 2022-12-09 12:04:11 | 0 |
|
pSCM167-Venus-VF-hSFRP1(aa181-314) Resource Report Resource Website |
RRID:Addgene_189550 | truncated secreted frizzled related protein 1 | Homo sapiens | Ampicillin | PMID: | Backbone Marker:Customized backbone from Fu lab ; Vector Backbone:pSCM167-Venus (Fu lab); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | contains aa181-314 | 2022-12-09 12:04:11 | 0 | |
|
pcDNA3-mCherry-TSC2 Resource Report Resource Website |
RRID:Addgene_192440 | mCherry-TSC2 | Homo sapiens | Ampicillin | PMID:35294906 | Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-12-09 12:04:16 | 0 |
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