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  • Organism:homo sapiens (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pERB258
 
Resource Report
Resource Website
RRID:Addgene_67760 Kif5b(560)-mCherry-eDHFR Homo sapiens Ampicillin PMID:25989077 2021-11-24 03:37:35 0
pDONR223_PIK3R1_WT
 
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RRID:Addgene_81748 PIK3R1 Homo sapiens Spectinomycin PMID:27147599 Open state: NO stop codon immediately follows insert Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin E181K 2022-12-10 12:07:09 0
pDONR223-PIK3R1
 
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RRID:Addgene_23696 PIK3R1 Homo sapiens Spectinomycin PMID:21107320 The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately. Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin 2022-12-10 12:04:30 0
pLenti4/V5-DEST-P85(T576del)
 
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RRID:Addgene_40228 P85(T576del) Homo sapiens Ampicillin PMID: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin deleted amino acid 576 2022-12-10 12:05:10 0
pLenti4/V5-DEST-P85(N564K)
 
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RRID:Addgene_40226 P85(N564K) Homo sapiens Ampicillin PMID: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin changed Asparagine 564 to Lysine 2022-12-10 12:05:10 0
pLenti4/V5-DEST-P85(K550_K562dup)
 
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RRID:Addgene_40224 P85(K550_K562dup) Homo sapiens Ampicillin PMID: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin created internal duplication of amino acids 550-562 2022-12-10 12:05:10 0
pFastBac1 PIK3R1-Ni
 
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RRID:Addgene_17744 PIK3R1-Ni Homo sapiens Ampicillin PMID:15950905 Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Partial cDNA 2022-12-10 12:03:41 0
pLenti4/V5-DEST-P85(KS459del)
 
Resource Report
Resource Website
RRID:Addgene_40222 P85(KS459delN) Homo sapiens Ampicillin PMID: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin amino acids 459-460 replaced with asparagine 2022-12-10 12:05:09 0
pLenti4/V5-DEST-P85(E439del)
 
Resource Report
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RRID:Addgene_40221 P85(E439del) Homo sapiens Ampicillin PMID: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin deleted amino acid 439 2022-12-10 12:05:09 0
pGL3-CMV-p85a 3'UTR mutant
 
Resource Report
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RRID:Addgene_51393 p85alpha 3'UTR Homo sapiens Ampicillin PMID:19079265 miR-29 seed mutant (TGCT->GTAG) Backbone Marker:Promega (modified to have 3' multi cloning sites); Vector Backbone:pGL3-UC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin miR-29 binding site mutant 2022-12-10 12:05:40 0
pcDNA3.1_iSH2-pMagFast2(3x)-iRFP
 
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1+ mentions
RRID:Addgene_67298 iSH2-pMagFast2(3x)-iRFP Homo sapiens Ampicillin PMID:25708714 Backbone Marker:Invitrogen; Backbone Size:5354; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-12-10 12:06:25 1
pBSSK p85 alpha (delta BH)
 
Resource Report
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RRID:Addgene_13431 p85 alpha (delta BH) Homo sapiens Ampicillin PMID:16880400 see author's map for more information. Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin BH domain replaced by flag tag. 2022-12-10 12:01:56 0
pGEX p85a(N)-SH2
 
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RRID:Addgene_46464 p85a(N) Homo sapiens Ampicillin PMID:17588523 The molecular weight of the GST fusion protein is approximately 38.5 kDa Backbone Marker:GE Healthcare Biosciences; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin contains amino acids 321-434 2022-12-10 12:05:26 0
pGEX p85a(C)-SH2
 
Resource Report
Resource Website
RRID:Addgene_46463 p85a(C) Homo sapiens Ampicillin PMID:17588523 The molecular weight of the GST fusion protein is approximately 38.4 kDa Backbone Marker:GE Healthcare Biosciences; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin contains amino acids 612-724 2022-12-10 12:05:26 0
pCDNA3-EMTB-TurboID-V5
 
Resource Report
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RRID:Addgene_190736 ensconsin Homo sapiens Ampicillin PMID:35952650 Backbone Size:5403; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Microtubule binding-domain of ensconsin (amino acids 18-282 of GenBank reference sequence X73882) 2022-12-10 12:04:13 0
pcDNA3-mCherry-Rheb(C181S)
 
Resource Report
Resource Website
RRID:Addgene_192437 mCherry-Rheb(C181S) Homo sapiens Ampicillin PMID:35294906 Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Rheb Cys 181 mutated to Ser; abolishes C-terminal farnesylation. 2022-12-09 12:04:16 0
pcDNA3-mCherry-TSC2(AA,3Q)-NLS
 
Resource Report
Resource Website
RRID:Addgene_192445 mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS Homo sapiens Ampicillin PMID:35294906 Backbone Size:5472; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in isoform 1) mutated to Ala; Lys 1614, Lys 1615, and Arg 1616 mutated to Gln. 2022-12-09 12:04:17 0
pSCM167-Venus-VF-hADAM10(aa220-459)
 
Resource Report
Resource Website
RRID:Addgene_189555 truncated ADAM metallopeptidase domain 10 Homo sapiens Ampicillin PMID: Please note: Plasmid contains a Y316H mutation in hADAM10 compared to the NCBI reference sequence NP_001101.1. This mutation was present in the original DNA that was was PCR amplified from human placenta cDNA and is not known to affect plasmid function. Backbone Marker:Customized backbone from Fu lab ; Vector Backbone:pSCM167-Venus (Fu lab); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin contains aa 220-459 2022-12-09 12:04:11 0
pSCM167-Venus-VF-hSFRP1(aa181-314)
 
Resource Report
Resource Website
RRID:Addgene_189550 truncated secreted frizzled related protein 1 Homo sapiens Ampicillin PMID: Backbone Marker:Customized backbone from Fu lab ; Vector Backbone:pSCM167-Venus (Fu lab); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin contains aa181-314 2022-12-09 12:04:11 0
pcDNA3-mCherry-TSC2
 
Resource Report
Resource Website
RRID:Addgene_192440 mCherry-TSC2 Homo sapiens Ampicillin PMID:35294906 Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-12-09 12:04:16 0

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