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  • Organism:drosophila melanogaster (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
UAS-dsarm(E)
 
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RRID:Addgene_187870 dSarm isoform E Drosophila melanogaster Ampicillin PMID:36476387 Backbone Size:9000; Vector Backbone:pCASPR (attB); Vector Types:; Bacterial Resistance:Ampicillin 2022-12-14 12:04:14 0
KHBD00026
 
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RRID:Addgene_34188 CG8333 Drosophila melanogaster Kanamycin PMID:22037703 Please note that the ORFs in this collection were cloned open-ended (without a stop codon). Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Other, Gateway Donor Vector; Bacterial Resistance:Kanamycin 2022-12-04 12:04:38 0
pET11-PC(75-390)-H6
 
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RRID:Addgene_183781 Polycomb Drosophila melanogaster Ampicillin PMID:26802126 Backbone Size:5680; Vector Backbone:pET-11d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 6 His tag at the C-terminus 2022-12-04 12:03:56 0
pUASTP2.1
 
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RRID:Addgene_13842 mini-white Drosophila melanogaster Ampicillin PMID:16547094 pUASTP2.1 contains a P-element target for RMCE, the mini-white gene flanked by inverted attP sites. It is a derivative of PUASTP2 in which the UAS and TATA sequences flanking the cassette have been removed. (Specifically, PUASTP2 was digested with BamHI and StuI to remove UAS-TATA-attP, and an attP-containing BamHI-EcoRV fragment from pTA-attP was inserted). This vector can be used to generate target lines by standard P element transgenesis. Backbone Size:4300; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2022-12-04 12:02:08 0
pUASTP2
 
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RRID:Addgene_13843 mini-white Drosophila melanogaster Ampicillin PMID:16547094 pUASTP2 contains a P-element target for RMCE, the mini-white gene flanked by inverted attP sites. This target cassette was contructed in the parent vector pUAST. This vector can be used to generate target lines by standard P element transgenesis. Please note that the UAS binding sites and hsp70 promoter of pUAST are outside of the exchange cassette in this vector - i.e. following RMCE, these elements will remain flanking the cassette. Backbone Size:5500; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2022-12-04 12:02:08 0
CS2-HA-dASUN
 
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RRID:Addgene_47041 Asunder Drosophila melanogaster Ampicillin PMID:23097494 Vector Backbone:CS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-12-03 12:05:29 0
Rae1-pDONR221
 
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RRID:Addgene_124240 Drosophila Rae1 Drosophila melanogaster Kanamycin PMID:28554770 Backbone Size:2563; Vector Backbone:pDONR221; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin 2022-04-22 03:21:53 0
pCFD3.1-w-dU6:3gRNA
 
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RRID:Addgene_123366 U6:3-gRNA Drosophila melanogaster Ampicillin PMID: When describing the use of this plasmid in publications, please state that it is derived from pCFD3 and cite: Port et al. Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):E2967-76. doi: 10.1073/pnas.1405500111 In pCFD3.1, the BbsI sites have been mutated in mini-white, which allows use of the remaining BbsI sites for gRNA cloning (using the protocol for pCFD3 (https://www.crisprflydesign.org/plasmids/)). Backbone Marker:Fillip Port; Vector Backbone:pCFD3-dU6-3gRNA (Addgene 49410); Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin 2022-04-22 03:21:27 0
K401-iLid
 
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RRID:Addgene_122484 Kinesin 1-401 iLid His tag Drosophila melanogaster Ampicillin PMID:31391558 Backbone Size:2500; Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Kinesin amino acids 1-401 2022-04-22 03:21:10 0
pcDNA3.1(-)-mRho.V5.mER.hOr47a
 
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RRID:Addgene_126472 Or47a Drosophila melanogaster Ampicillin PMID:31504297 Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin codon optimization for H. sapiens 2022-04-22 03:22:26 0
pcDNA3.1(-)-V5.mER.hOr47a
 
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RRID:Addgene_126473 Or47a Drosophila melanogaster Ampicillin PMID:31504297 Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin codon optimization for H. sapiens 2022-04-22 03:22:26 0
pDmelOR
 
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RRID:Addgene_126476 DmelOrco Drosophila melanogaster Ampicillin PMID:31504297 Is it recommended to digest the vector with XhoI and PvuI-HF and gel purify the two bands before sequencing as the two CMV promoters and SV40-polyA sequences are very similar to each other. Use the EcoRI restriction site for best functional expression on the MCS2. Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint Backbone Size:4111; Vector Backbone:pCMV-BI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin codon optimization for H. sapiens; includes a recombinant β-globin/IgG chimeric intron to inhibit its functional expression in bacteria 2022-04-22 03:22:26 0
pDmelOR-mRho.V5.mER.hOr47a
 
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RRID:Addgene_126478 Or47a Drosophila melanogaster Ampicillin PMID:31504297 Is it recommended to digest the vector with XhoI and NaeI and gel purify the two bands before sequencing as the two CMV promoters and SV40-polyA sequences are very similar to each other Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint Backbone Size:4111; Vector Backbone:pCMV-BI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin codon optimization for H. sapiens 2022-04-22 03:22:26 0
pDmelOR-mRho.V5.mER.hOr56a
 
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RRID:Addgene_126479 Or56a Drosophila melanogaster Ampicillin PMID:31504297 Is it recommended to digest the vector with XhoI and NaeI and gel purify the two bands before sequencing as the two CMV promoters and SV40-polyA sequences are very similar to each other Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint Backbone Size:4111; Vector Backbone:pCMV-BI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin codon optimization for H. sapiens 2022-04-22 03:22:26 0
pJC-mhc2.4kb-TALE3-VP64-P10
 
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RRID:Addgene_104611 2.4kb mhc enhancer element Drosophila melanogaster Ampicillin PMID:29162808 Backbone Marker:Tudor A. Fulga; Backbone Size:11291; Vector Backbone:pJC-20xUAS-TALE3-VP64-P10; Vector Types:Insect Expression, TALEN; Bacterial Resistance:Ampicillin 2022-09-21 01:00:16 0
pJC-ase0.8kb-TALE3-VP64-P10
 
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RRID:Addgene_104610 0.8kb ase enhancer element Drosophila melanogaster Ampicillin PMID:29162808 Backbone Marker:Tudor A. Fulga; Backbone Size:11305; Vector Backbone:pJC-20xUAS-TALE3-VP64-P10; Vector Types:Insect Expression, TALEN; Bacterial Resistance:Ampicillin 2022-09-21 01:00:16 0
pJC-repo1.9kb-TALE4-VP64-P10
 
Resource Report
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RRID:Addgene_104612 1.9kb repo enhancer element Drosophila melanogaster Ampicillin PMID:29162808 Backbone Marker:Tudor A. Fulga; Backbone Size:11333; Vector Backbone:pJC-20xUAS-TALE4-VP64-P10; Vector Types:Insect Expression, TALEN; Bacterial Resistance:Ampicillin 2022-09-21 01:00:16 0
pUAST-Myc-dATG1
 
Resource Report
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RRID:Addgene_111801 dAtg1 Drosophila melanogaster Ampicillin PMID:23819996 Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2022-11-19 12:00:32 0
pBABR IRFP-Bcd-LEXY
 
Resource Report
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RRID:Addgene_182595 Bcd Drosophila melanogaster Ampicillin PMID:35320726 Vector Backbone:pBABR; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2022-11-22 12:19:48 0
KHBD00188
 
Resource Report
Resource Website
RRID:Addgene_34340 CG1034 Drosophila melanogaster Kanamycin PMID:22037703 Please note that the ORFs in this collection were cloned open-ended (without a stop codon). Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Other, Gateway Donor Vector; Bacterial Resistance:Kanamycin 2022-11-22 12:04:48 0

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