| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
UAS-dsarm(E) Resource Report Resource Website |
RRID:Addgene_187870 | dSarm isoform E | Drosophila melanogaster | Ampicillin | PMID:36476387 | Backbone Size:9000; Vector Backbone:pCASPR (attB); Vector Types:; Bacterial Resistance:Ampicillin | 2022-12-14 12:04:14 | 0 | ||
|
KHBD00026 Resource Report Resource Website |
RRID:Addgene_34188 | CG8333 | Drosophila melanogaster | Kanamycin | PMID:22037703 | Please note that the ORFs in this collection were cloned open-ended (without a stop codon). | Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Other, Gateway Donor Vector; Bacterial Resistance:Kanamycin | 2022-12-04 12:04:38 | 0 | |
|
pET11-PC(75-390)-H6 Resource Report Resource Website |
RRID:Addgene_183781 | Polycomb | Drosophila melanogaster | Ampicillin | PMID:26802126 | Backbone Size:5680; Vector Backbone:pET-11d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 6 His tag at the C-terminus | 2022-12-04 12:03:56 | 0 | |
|
pUASTP2.1 Resource Report Resource Website |
RRID:Addgene_13842 | mini-white | Drosophila melanogaster | Ampicillin | PMID:16547094 | pUASTP2.1 contains a P-element target for RMCE, the mini-white gene flanked by inverted attP sites. It is a derivative of PUASTP2 in which the UAS and TATA sequences flanking the cassette have been removed. (Specifically, PUASTP2 was digested with BamHI and StuI to remove UAS-TATA-attP, and an attP-containing BamHI-EcoRV fragment from pTA-attP was inserted). This vector can be used to generate target lines by standard P element transgenesis. | Backbone Size:4300; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2022-12-04 12:02:08 | 0 | |
|
pUASTP2 Resource Report Resource Website |
RRID:Addgene_13843 | mini-white | Drosophila melanogaster | Ampicillin | PMID:16547094 | pUASTP2 contains a P-element target for RMCE, the mini-white gene flanked by inverted attP sites. This target cassette was contructed in the parent vector pUAST. This vector can be used to generate target lines by standard P element transgenesis. Please note that the UAS binding sites and hsp70 promoter of pUAST are outside of the exchange cassette in this vector - i.e. following RMCE, these elements will remain flanking the cassette. | Backbone Size:5500; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2022-12-04 12:02:08 | 0 | |
|
CS2-HA-dASUN Resource Report Resource Website |
RRID:Addgene_47041 | Asunder | Drosophila melanogaster | Ampicillin | PMID:23097494 | Vector Backbone:CS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-12-03 12:05:29 | 0 | ||
|
Rae1-pDONR221 Resource Report Resource Website |
RRID:Addgene_124240 | Drosophila Rae1 | Drosophila melanogaster | Kanamycin | PMID:28554770 | Backbone Size:2563; Vector Backbone:pDONR221; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin | 2022-04-22 03:21:53 | 0 | ||
|
pCFD3.1-w-dU6:3gRNA Resource Report Resource Website |
RRID:Addgene_123366 | U6:3-gRNA | Drosophila melanogaster | Ampicillin | PMID: | When describing the use of this plasmid in publications, please state that it is derived from pCFD3 and cite: Port et al. Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):E2967-76. doi: 10.1073/pnas.1405500111 In pCFD3.1, the BbsI sites have been mutated in mini-white, which allows use of the remaining BbsI sites for gRNA cloning (using the protocol for pCFD3 (https://www.crisprflydesign.org/plasmids/)). | Backbone Marker:Fillip Port; Vector Backbone:pCFD3-dU6-3gRNA (Addgene 49410); Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin | 2022-04-22 03:21:27 | 0 | |
|
K401-iLid Resource Report Resource Website |
RRID:Addgene_122484 | Kinesin 1-401 iLid His tag | Drosophila melanogaster | Ampicillin | PMID:31391558 | Backbone Size:2500; Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Kinesin amino acids 1-401 | 2022-04-22 03:21:10 | 0 | |
|
pcDNA3.1(-)-mRho.V5.mER.hOr47a Resource Report Resource Website |
RRID:Addgene_126472 | Or47a | Drosophila melanogaster | Ampicillin | PMID:31504297 | Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint | Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | codon optimization for H. sapiens | 2022-04-22 03:22:26 | 0 |
|
pcDNA3.1(-)-V5.mER.hOr47a Resource Report Resource Website |
RRID:Addgene_126473 | Or47a | Drosophila melanogaster | Ampicillin | PMID:31504297 | Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint | Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | codon optimization for H. sapiens | 2022-04-22 03:22:26 | 0 |
|
pDmelOR Resource Report Resource Website |
RRID:Addgene_126476 | DmelOrco | Drosophila melanogaster | Ampicillin | PMID:31504297 | Is it recommended to digest the vector with XhoI and PvuI-HF and gel purify the two bands before sequencing as the two CMV promoters and SV40-polyA sequences are very similar to each other. Use the EcoRI restriction site for best functional expression on the MCS2. Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint | Backbone Size:4111; Vector Backbone:pCMV-BI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | codon optimization for H. sapiens; includes a recombinant β-globin/IgG chimeric intron to inhibit its functional expression in bacteria | 2022-04-22 03:22:26 | 0 |
|
pDmelOR-mRho.V5.mER.hOr47a Resource Report Resource Website |
RRID:Addgene_126478 | Or47a | Drosophila melanogaster | Ampicillin | PMID:31504297 | Is it recommended to digest the vector with XhoI and NaeI and gel purify the two bands before sequencing as the two CMV promoters and SV40-polyA sequences are very similar to each other Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint | Backbone Size:4111; Vector Backbone:pCMV-BI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | codon optimization for H. sapiens | 2022-04-22 03:22:26 | 0 |
|
pDmelOR-mRho.V5.mER.hOr56a Resource Report Resource Website |
RRID:Addgene_126479 | Or56a | Drosophila melanogaster | Ampicillin | PMID:31504297 | Is it recommended to digest the vector with XhoI and NaeI and gel purify the two bands before sequencing as the two CMV promoters and SV40-polyA sequences are very similar to each other Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint | Backbone Size:4111; Vector Backbone:pCMV-BI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | codon optimization for H. sapiens | 2022-04-22 03:22:26 | 0 |
|
pJC-mhc2.4kb-TALE3-VP64-P10 Resource Report Resource Website |
RRID:Addgene_104611 | 2.4kb mhc enhancer element | Drosophila melanogaster | Ampicillin | PMID:29162808 | Backbone Marker:Tudor A. Fulga; Backbone Size:11291; Vector Backbone:pJC-20xUAS-TALE3-VP64-P10; Vector Types:Insect Expression, TALEN; Bacterial Resistance:Ampicillin | 2022-09-21 01:00:16 | 0 | ||
|
pJC-ase0.8kb-TALE3-VP64-P10 Resource Report Resource Website |
RRID:Addgene_104610 | 0.8kb ase enhancer element | Drosophila melanogaster | Ampicillin | PMID:29162808 | Backbone Marker:Tudor A. Fulga; Backbone Size:11305; Vector Backbone:pJC-20xUAS-TALE3-VP64-P10; Vector Types:Insect Expression, TALEN; Bacterial Resistance:Ampicillin | 2022-09-21 01:00:16 | 0 | ||
|
pJC-repo1.9kb-TALE4-VP64-P10 Resource Report Resource Website |
RRID:Addgene_104612 | 1.9kb repo enhancer element | Drosophila melanogaster | Ampicillin | PMID:29162808 | Backbone Marker:Tudor A. Fulga; Backbone Size:11333; Vector Backbone:pJC-20xUAS-TALE4-VP64-P10; Vector Types:Insect Expression, TALEN; Bacterial Resistance:Ampicillin | 2022-09-21 01:00:16 | 0 | ||
|
pUAST-Myc-dATG1 Resource Report Resource Website |
RRID:Addgene_111801 | dAtg1 | Drosophila melanogaster | Ampicillin | PMID:23819996 | Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2022-11-19 12:00:32 | 0 | ||
|
pBABR IRFP-Bcd-LEXY Resource Report Resource Website |
RRID:Addgene_182595 | Bcd | Drosophila melanogaster | Ampicillin | PMID:35320726 | Vector Backbone:pBABR; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2022-11-22 12:19:48 | 0 | ||
|
KHBD00188 Resource Report Resource Website |
RRID:Addgene_34340 | CG1034 | Drosophila melanogaster | Kanamycin | PMID:22037703 | Please note that the ORFs in this collection were cloned open-ended (without a stop codon). | Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Other, Gateway Donor Vector; Bacterial Resistance:Kanamycin | 2022-11-22 12:04:48 | 0 |
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