Searching the RRID Resource Information Network
Species: Danio rerio
Genetic Insert: Zebrafish Wnt3 promoter
Vector Backbone Description: Vector Backbone:pGL3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192992 Copy
Species: Danio rerio
Genetic Insert: Zebrafish Sp5-like
Vector Backbone Description: Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192999 Copy
Species: Danio rerio
Genetic Insert: myelin basic protein
Vector Backbone Description: Backbone Size:4308; Vector Backbone:pZL1; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103007 Copy
Species: Danio rerio
Genetic Insert: Claudin 15-like a
Vector Backbone Description: Backbone Size:2663; Vector Backbone:p5E-Fse-Asc 381; Vector Types:Other; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_125026 Copy
Species: Danio rerio
Genetic Insert: ApoA4a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCRII; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121902 Copy
Species: Danio rerio
Genetic Insert: ApoBa
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCRII; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121897 Copy
Species: Danio rerio
Genetic Insert: ApoBb.1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCRII; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121898 Copy
Species: Danio rerio
Genetic Insert: kank4
Vector Backbone Description: Vector Backbone:pJet1.2; Vector Types:Other, Cloning Vector; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121982 Copy
Species: Danio rerio
Genetic Insert: kank1a
Vector Backbone Description: Vector Backbone:pJet1.2; Vector Types:Other, Cloning Vector; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121978 Copy
Species: Danio rerio
Genetic Insert: kank1b
Vector Backbone Description: Vector Backbone:pJet1.2; Vector Types:Other, Cloning Vector; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121979 Copy
Species: Danio rerio
Genetic Insert: mfn2 gRNA
Vector Backbone Description: Backbone Marker:Tol2 kit; Backbone Size:4000; Vector Backbone:p3E; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121994 Copy
Species: Danio rerio
Genetic Insert: opa1 gRNA
Vector Backbone Description: Backbone Marker:Tol2 kit; Backbone Size:4000; Vector Backbone:p3E; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121995 Copy
Species: Danio rerio
Genetic Insert: Fgf2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_12309 Copy
Species: Danio rerio
Genetic Insert: dre-D145N CDK2 DN
Vector Backbone Description: Backbone Size:8500; Vector Backbone:tol2 insertion; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121132 Copy
Species: Danio rerio
Genetic Insert: Trpa1b-FLAG
Vector Backbone Description: Backbone Size:4275; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_106403 Copy
Species: Danio rerio
Genetic Insert: Dnase1l1l-mRFP
Vector Backbone Description: Backbone Size:4120; Vector Backbone:pCS2; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_169002 Copy
Species: Danio rerio
Genetic Insert: miR-126
Vector Backbone Description: Backbone Size:0; Vector Backbone:pME; Vector Types:Other, Entry vector; Bacterial Resistance:Kanamycin
References:
Comments: The insert includes genomic sequence flanking the mature miR-126 sequence on chromosome 8 (miRBase accession number MI0001979, Zv8).
There is a C->T mutation in the miR126. The mutation is probably due to a SNP and should not affect plasmid function.
Proper citation: RRID:Addgene_26033 Copy
Species: Danio rerio
Genetic Insert: Cavin1a
Vector Backbone Description: Vector Backbone:pDest-Tol2-pA2; Vector Types:Other, Zebrafish expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163852 Copy
Species: Danio rerio
Genetic Insert: flk1
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments: For riboprobe, digest with EcoRI and use T7 polymerase.
Proper citation: RRID:Addgene_13061 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting PTRF/Cavin1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Other, Zebrafish Targeting; Bacterial Resistance:Kanamycin
References:
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene PTRF/Cavin1 (see below). Please note that this plasmid does NOT contain the PTRF/Cavin1 sequence.
Users must order the complementary plasmid PTRF/Cavin1_L (OZ513) [Addgene plasmid 27188] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-GGAGGTGCC-3' in a bacterial two hybrid assay, and resulted in 6.81 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify zebrafishzfs@zincfingers.org and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles:
"Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_27189 Copy
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