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  • Organism:danio rerio (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
ZfWnt3:Luc
 
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RRID:Addgene_192992 Zebrafish Wnt3 promoter Danio rerio Ampicillin PMID:30659200 Vector Backbone:pGL3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-12-04 12:04:11 0
ZfSp5l1
 
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RRID:Addgene_192999 Zebrafish Sp5-like Danio rerio Ampicillin PMID:30659200 Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-12-04 12:04:11 0
pZL1-ZF - myelin basic protein a (mbpa)
 
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RRID:Addgene_103007 myelin basic protein Danio rerio Ampicillin PMID:12112375 Backbone Size:4308; Vector Backbone:pZL1; Vector Types:; Bacterial Resistance:Ampicillin 2022-04-22 03:15:24 0
p5E-0.349cldn15la
 
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RRID:Addgene_125026 Claudin 15-like a Danio rerio Kanamycin PMID:30845179 Backbone Size:2663; Vector Backbone:p5E-Fse-Asc 381; Vector Types:Other; Bacterial Resistance:Kanamycin 2022-04-22 03:22:05 0
PCRII apoA-IVa
 
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RRID:Addgene_121902 ApoA4a Danio rerio Ampicillin and Kanamycin PMID:25633982 Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCRII; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin 2022-04-22 03:21:00 0
PCRII apoBa
 
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RRID:Addgene_121897 ApoBa Danio rerio Ampicillin and Kanamycin PMID:25633982 Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCRII; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin 2022-04-22 03:21:00 0
PCRII apoBb.1
 
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RRID:Addgene_121898 ApoBb.1 Danio rerio Ampicillin and Kanamycin PMID:25633982 Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCRII; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin 2022-04-22 03:21:00 0
kank4_pJet1.2
 
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RRID:Addgene_121982 kank4 Danio rerio Ampicillin PMID:27292017 Vector Backbone:pJet1.2; Vector Types:Other, Cloning Vector; Bacterial Resistance:Ampicillin 2022-04-22 03:21:02 0
kank1a_pJet1.2
 
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RRID:Addgene_121978 kank1a Danio rerio Ampicillin PMID:27292017 Vector Backbone:pJet1.2; Vector Types:Other, Cloning Vector; Bacterial Resistance:Ampicillin 2022-04-22 03:21:02 0
kank1b_pJet1.2
 
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RRID:Addgene_121979 kank1b Danio rerio Ampicillin PMID:27292017 Vector Backbone:pJet1.2; Vector Types:Other, Cloning Vector; Bacterial Resistance:Ampicillin 2022-04-22 03:21:02 0
p3E-U6a-U6c-mfn2 guide#2
 
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RRID:Addgene_121994 mfn2 gRNA Danio rerio Kanamycin PMID:32788232 Backbone Marker:Tol2 kit; Backbone Size:4000; Vector Backbone:p3E; Vector Types:CRISPR; Bacterial Resistance:Kanamycin 2022-04-22 03:21:02 0
p3E-U6a-U6c-opa1 guide
 
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RRID:Addgene_121995 opa1 gRNA Danio rerio Kanamycin PMID:32788232 Backbone Marker:Tol2 kit; Backbone Size:4000; Vector Backbone:p3E; Vector Types:CRISPR; Bacterial Resistance:Kanamycin 2022-04-22 03:21:02 0
zbFGF
 
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1+ mentions
RRID:Addgene_12309 Fgf2 Danio rerio Kanamycin PMID:16862139 Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Ala 11 mutated to Pro, Ser 55 mutated to Asn 2022-04-22 03:21:20 1
Tol2-lyzC-mcherry-2a-CDK2-D145N
 
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RRID:Addgene_121132 dre-D145N CDK2 DN Danio rerio Ampicillin PMID:31451657 Backbone Size:8500; Vector Backbone:tol2 insertion; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin D145N point mutation DN 2022-04-22 03:20:49 0
pCMV-Trpa1b-FLAG
 
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RRID:Addgene_106403 Trpa1b-FLAG Danio rerio Kanamycin PMID:28928472 Backbone Size:4275; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2022-09-21 01:00:19 0
pCS2-Dnase1l1l-mRFP
 
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RRID:Addgene_169002 Dnase1l1l-mRFP Danio rerio Ampicillin PMID:33854238 Backbone Size:4120; Vector Backbone:pCS2; Vector Types:Unspecified; Bacterial Resistance:Ampicillin 2022-09-21 01:02:11 0
776 pME-miR-126
 
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RRID:Addgene_26033 miR-126 Danio rerio Kanamycin PMID:20364122 The insert includes genomic sequence flanking the mature miR-126 sequence on chromosome 8 (miRBase accession number MI0001979, Zv8). There is a C->T mutation in the miR126. The mutation is probably due to a SNP and should not affect plasmid function. Backbone Size:0; Vector Backbone:pME; Vector Types:Other, Entry vector; Bacterial Resistance:Kanamycin 2022-09-15 01:02:59 0
acta1-Cavin1a-Clover
 
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RRID:Addgene_163852 Cavin1a Danio rerio Ampicillin PMID:33591275 Vector Backbone:pDest-Tol2-pA2; Vector Types:Other, Zebrafish expression; Bacterial Resistance:Ampicillin 2022-11-11 12:02:45 0
36 p flk1
 
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1+ mentions
RRID:Addgene_13061 flk1 Danio rerio Ampicillin PMID:11585794 For riboprobe, digest with EcoRI and use T7 polymerase. Backbone Size:0; Vector Backbone:na; Vector Types:; Bacterial Resistance:Ampicillin 2022-11-11 12:01:44 1
PTRF/Cavin1_R (OZ514)
 
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RRID:Addgene_27189 Zinc finger array targeting PTRF/Cavin1 Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene PTRF/Cavin1 (see below). Please note that this plasmid does NOT contain the PTRF/Cavin1 sequence. Users must order the complementary plasmid PTRF/Cavin1_L (OZ513) [Addgene plasmid 27188] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGAGGTGCC-3' in a bacterial two hybrid assay, and resulted in 6.81 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify zebrafishzfs@zincfingers.org and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Other, Zebrafish Targeting; Bacterial Resistance:Kanamycin 2022-11-11 12:03:49 0

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