| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
ZfWnt3:Luc Resource Report Resource Website |
RRID:Addgene_192992 | Zebrafish Wnt3 promoter | Danio rerio | Ampicillin | PMID:30659200 | Vector Backbone:pGL3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-12-04 12:04:11 | 0 | ||
|
ZfSp5l1 Resource Report Resource Website |
RRID:Addgene_192999 | Zebrafish Sp5-like | Danio rerio | Ampicillin | PMID:30659200 | Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-12-04 12:04:11 | 0 | ||
|
pZL1-ZF - myelin basic protein a (mbpa) Resource Report Resource Website |
RRID:Addgene_103007 | myelin basic protein | Danio rerio | Ampicillin | PMID:12112375 | Backbone Size:4308; Vector Backbone:pZL1; Vector Types:; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:24 | 0 | ||
|
p5E-0.349cldn15la Resource Report Resource Website |
RRID:Addgene_125026 | Claudin 15-like a | Danio rerio | Kanamycin | PMID:30845179 | Backbone Size:2663; Vector Backbone:p5E-Fse-Asc 381; Vector Types:Other; Bacterial Resistance:Kanamycin | 2022-04-22 03:22:05 | 0 | ||
|
PCRII apoA-IVa Resource Report Resource Website |
RRID:Addgene_121902 | ApoA4a | Danio rerio | Ampicillin and Kanamycin | PMID:25633982 | Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCRII; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin | 2022-04-22 03:21:00 | 0 | ||
|
PCRII apoBa Resource Report Resource Website |
RRID:Addgene_121897 | ApoBa | Danio rerio | Ampicillin and Kanamycin | PMID:25633982 | Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCRII; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin | 2022-04-22 03:21:00 | 0 | ||
|
PCRII apoBb.1 Resource Report Resource Website |
RRID:Addgene_121898 | ApoBb.1 | Danio rerio | Ampicillin and Kanamycin | PMID:25633982 | Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCRII; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin | 2022-04-22 03:21:00 | 0 | ||
|
kank4_pJet1.2 Resource Report Resource Website |
RRID:Addgene_121982 | kank4 | Danio rerio | Ampicillin | PMID:27292017 | Vector Backbone:pJet1.2; Vector Types:Other, Cloning Vector; Bacterial Resistance:Ampicillin | 2022-04-22 03:21:02 | 0 | ||
|
kank1a_pJet1.2 Resource Report Resource Website |
RRID:Addgene_121978 | kank1a | Danio rerio | Ampicillin | PMID:27292017 | Vector Backbone:pJet1.2; Vector Types:Other, Cloning Vector; Bacterial Resistance:Ampicillin | 2022-04-22 03:21:02 | 0 | ||
|
kank1b_pJet1.2 Resource Report Resource Website |
RRID:Addgene_121979 | kank1b | Danio rerio | Ampicillin | PMID:27292017 | Vector Backbone:pJet1.2; Vector Types:Other, Cloning Vector; Bacterial Resistance:Ampicillin | 2022-04-22 03:21:02 | 0 | ||
|
p3E-U6a-U6c-mfn2 guide#2 Resource Report Resource Website |
RRID:Addgene_121994 | mfn2 gRNA | Danio rerio | Kanamycin | PMID:32788232 | Backbone Marker:Tol2 kit; Backbone Size:4000; Vector Backbone:p3E; Vector Types:CRISPR; Bacterial Resistance:Kanamycin | 2022-04-22 03:21:02 | 0 | ||
|
p3E-U6a-U6c-opa1 guide Resource Report Resource Website |
RRID:Addgene_121995 | opa1 gRNA | Danio rerio | Kanamycin | PMID:32788232 | Backbone Marker:Tol2 kit; Backbone Size:4000; Vector Backbone:p3E; Vector Types:CRISPR; Bacterial Resistance:Kanamycin | 2022-04-22 03:21:02 | 0 | ||
|
zbFGF Resource Report Resource Website 1+ mentions |
RRID:Addgene_12309 | Fgf2 | Danio rerio | Kanamycin | PMID:16862139 | Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Ala 11 mutated to Pro, Ser 55 mutated to Asn | 2022-04-22 03:21:20 | 1 | |
|
Tol2-lyzC-mcherry-2a-CDK2-D145N Resource Report Resource Website |
RRID:Addgene_121132 | dre-D145N CDK2 DN | Danio rerio | Ampicillin | PMID:31451657 | Backbone Size:8500; Vector Backbone:tol2 insertion; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | D145N point mutation DN | 2022-04-22 03:20:49 | 0 | |
|
pCMV-Trpa1b-FLAG Resource Report Resource Website |
RRID:Addgene_106403 | Trpa1b-FLAG | Danio rerio | Kanamycin | PMID:28928472 | Backbone Size:4275; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2022-09-21 01:00:19 | 0 | ||
|
pCS2-Dnase1l1l-mRFP Resource Report Resource Website |
RRID:Addgene_169002 | Dnase1l1l-mRFP | Danio rerio | Ampicillin | PMID:33854238 | Backbone Size:4120; Vector Backbone:pCS2; Vector Types:Unspecified; Bacterial Resistance:Ampicillin | 2022-09-21 01:02:11 | 0 | ||
|
776 pME-miR-126 Resource Report Resource Website |
RRID:Addgene_26033 | miR-126 | Danio rerio | Kanamycin | PMID:20364122 | The insert includes genomic sequence flanking the mature miR-126 sequence on chromosome 8 (miRBase accession number MI0001979, Zv8). There is a C->T mutation in the miR126. The mutation is probably due to a SNP and should not affect plasmid function. | Backbone Size:0; Vector Backbone:pME; Vector Types:Other, Entry vector; Bacterial Resistance:Kanamycin | 2022-09-15 01:02:59 | 0 | |
|
acta1-Cavin1a-Clover Resource Report Resource Website |
RRID:Addgene_163852 | Cavin1a | Danio rerio | Ampicillin | PMID:33591275 | Vector Backbone:pDest-Tol2-pA2; Vector Types:Other, Zebrafish expression; Bacterial Resistance:Ampicillin | 2022-11-11 12:02:45 | 0 | ||
|
36 p flk1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_13061 | flk1 | Danio rerio | Ampicillin | PMID:11585794 | For riboprobe, digest with EcoRI and use T7 polymerase. | Backbone Size:0; Vector Backbone:na; Vector Types:; Bacterial Resistance:Ampicillin | 2022-11-11 12:01:44 | 1 | |
|
PTRF/Cavin1_R (OZ514) Resource Report Resource Website |
RRID:Addgene_27189 | Zinc finger array targeting PTRF/Cavin1 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene PTRF/Cavin1 (see below). Please note that this plasmid does NOT contain the PTRF/Cavin1 sequence. Users must order the complementary plasmid PTRF/Cavin1_L (OZ513) [Addgene plasmid 27188] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGAGGTGCC-3' in a bacterial two hybrid assay, and resulted in 6.81 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify zebrafishzfs@zincfingers.org and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Other, Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2022-11-11 12:03:49 | 0 |
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