| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
pCRZeroT Resource Report Resource Website |
RRID:Addgene_120276 | Other | PMID:31015513 | Vector Backbone:pUC18; Vector Types:Other; Bacterial Resistance:Other | 2022-04-22 03:20:37 | 0 | ||||
|
pLD1 translation factors Resource Report Resource Website |
RRID:Addgene_117760 | Translation factor cistrons 1-13 | Other | Other | PMID:28977654 | The plasmid can be prone to recombination. The depositing lab suggests selecting multiple clones for restriction digest analysis as described in the publication before proceeding. | Backbone Size:5018; Vector Backbone:BioBrick pET-24a(+); Vector Types:Other, Escherichia coli; Bacterial Resistance:Other | 2022-04-22 03:19:40 | 0 | |
|
R6K_tac_mCherry_ChInt Resource Report Resource Website |
RRID:Addgene_187382 | mCherry | Synthetic | Other | PMID: | This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration). | Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2022-09-08 01:02:33 | 0 | |
|
pKD4_mCherry Resource Report Resource Website |
RRID:Addgene_187386 | mCherry | Synthetic | Other | PMID: | This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration). | Vector Backbone:pkD4; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2022-09-10 01:02:34 | 0 | |
|
pOD_009 Resource Report Resource Website |
RRID:Addgene_159520 | Other | PMID:35377874 | - The destination strain should be recA+ and not be able to maintain R6K plasmid - Zeocin is used to select colonies with the integrated vector. - The rhaBAD and lac promoters can be induced with 0.2% rhamnose and 0.5mM IPTG | Backbone Marker:Ilya B. Tikh and James C. Samuelson1; Backbone Size:4282; Vector Backbone:pDEL; Vector Types:Synthetic Biology; Bacterial Resistance:Other | 2022-10-07 04:51:26 | 0 | |||
|
S1030 Resource Report Resource Website |
RRID:Addgene_105063 | none | Other | PMID:24487694 | S1030 Genotype: F’ proA+B+ Δ(lacIZY) zzf::Tn10 lacIQ1 PN25-tetR luxCDE / endA1 recA1 galE15 galK16 nupG rpsL ΔlacIZYA araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) proBA::pir116 araE201 ΔrpoZ Δflu ΔcsgABCDEFG ΔpgaC λ– | Vector Backbone:none; Vector Types:; Bacterial Resistance:Other | 2022-04-22 03:15:53 | 0 | ||
|
pF2-DTA-Rosa26 Resource Report Resource Website |
RRID:Addgene_42522 | Rosa26 genomic fragment | Mus musculus | Other | PMID:23437314 | Vector Backbone:pF2; Vector Types:Mouse Targeting; Bacterial Resistance:Other | 2022-04-22 03:40:45 | 0 | ||
|
pBMTB-6 Resource Report Resource Website |
RRID:Addgene_22823 | Other | PMID:16496398 | Addgene's QC sequence shows a 1bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity. | Backbone Size:7044; Vector Backbone:pBMTB-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2022-04-22 03:36:08 | 0 | |||
|
pBMT-6 Resource Report Resource Website |
RRID:Addgene_22835 | Other | PMID:16496398 | Backbone Size:5862; Vector Backbone:pBMT-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2022-04-22 03:36:09 | 0 | ||||
|
pT2ST Resource Report Resource Website |
RRID:Addgene_59384 | TT-ISceI-dfrA | Synthetic | Other | PMID:25255806 |
iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The trimethoprim resistance gene was taken from EZ-Tn5™ |
Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:Other, template; Bacterial Resistance:Other | 2022-04-22 03:44:58 | 0 | |
|
pRL1058 Resource Report Resource Website |
RRID:Addgene_70686 | Transposon Tn5-1058 | Other | PMID:11607193 | Tn5 derivative Tn5-1058 (in plasmid pRL1058) contains a p15A oriV, and its antibiotic-resistance determinants are driven by an Amaranthus hybridus ribulose bisphosphate carboxylase promoter (see pRL439 is this series of plasmids), providing large numbers of transposon mutants. The XbaI site near the L end of Tn5 is the sole unmethylated XbaI in the construct, allowing the placement of luciferases (e.g., Vibrio fischeri luxAB as in pRL1063a). An oriT for conjugal transfer is positioned between the L and R ends of the Tn. | Vector Backbone:Transposon Tn5, variously modified; Vector Types:; Bacterial Resistance:Other | 2022-04-22 03:47:51 | 0 | ||
|
pAUC40 Resource Report Resource Website |
RRID:Addgene_71298 | Other | PMID:19843225 | Please note that there are several discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function. | Backbone Marker:Kaniga, et al Gene. 1991 109: 137-141.; Backbone Size:8509; Vector Backbone:pKNG101; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2022-04-22 03:48:00 | 0 | |||
|
MIP 227 CoMiP 4in1 wo shRNA p53 Resource Report Resource Website |
RRID:Addgene_63727 | Mini-intronic-plasmid intron | Homo sapiens | Other | PMID:25628230 | In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514. | Backbone Size:7608; Vector Backbone:codon-optimized minicircle (CoMiC) construct; Vector Types:Mammalian Expression; Bacterial Resistance:Other | codon-optimised | 2022-04-22 03:46:13 | 0 |
|
FW102 OL2-62 Resource Report Resource Website |
RRID:Addgene_53735 | none | Other | Other | PMID:9121589 | FW102 containing an F' Kan bearing the placOL2–62-lacZ fusion where the lambda-CI operator (OL2) is centered at position –62 relative to the start of transcription. This is the reporter strain used with the Hochschild lab bacterial 2-hybrid system, as described in the following article: http://www.addgene.org/browse/article/8393/ This strain is also described in Deighan, P., Diez, C.M., Leibman, M., Hochschild, A., and Nickels, B.E. (2008) The bacteriophage λ Q antiterminator protein contacts the β-flap domain of RNA polymerase, PNAS 105: 15305-15310 | Vector Backbone:none; Vector Types:; Bacterial Resistance:Other | 2022-04-22 03:43:35 | 0 | |
|
pEx-insertion-erm-T1T2 Resource Report Resource Website |
RRID:Addgene_172212 | Other | PMID:34676563 | Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at help@addgene.org or contact our distributors if you have any questions. | Backbone Marker:Koropatkin, N.M. (PMID: 18611383); Backbone Size:4172; Vector Backbone:pExchange-tdk; Vector Types:Other, Genetic insertion in Prevotella copri; Bacterial Resistance:Other | 2023-05-05 01:04:34 | 0 | |||
|
pFLAG_OriM Resource Report Resource Website |
RRID:Addgene_110097 | Other | PMID:29934571 | Vector Backbone:pFLAG; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2023-05-10 01:00:33 | 0 | ||||
|
pNR-DEST Resource Report Resource Website |
RRID:Addgene_90101 | Other | PMID: | Backbone Size:10902; Vector Backbone:pPtPBR1; Vector Types:Other, Gateway Destination vector for protein expression in diatoms; Bacterial Resistance:Other | 2023-05-10 01:06:39 | 0 | ||||
|
p49202-DEST Resource Report Resource Website |
RRID:Addgene_90100 | Other | PMID: | Backbone Size:11457; Vector Backbone:pPtPBR1; Vector Types:Other, Gateway Destination vector for protein expression in diatoms; Bacterial Resistance:Other | 2023-05-10 01:06:39 | 0 | ||||
|
pH4-DEST Resource Report Resource Website |
RRID:Addgene_90099 | Other | PMID: | Backbone Size:10591; Vector Backbone:pPtPBR1; Vector Types:Other, Gateway Destination vector for protein expression in diatoms; Bacterial Resistance:Other | 2023-05-10 01:06:39 | 0 | ||||
|
MIP 339 CoMiP coLin28 2A Nanog IRES eGFP Resource Report Resource Website |
RRID:Addgene_63729 | Lin28 | Homo sapiens | Other | PMID:25628230 | In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514. | Vector Backbone:no backbone--uses MiP intron for replication in bacteria; Vector Types:Mammalian Expression; Bacterial Resistance:Other | codon optimized | 2023-09-15 01:12:40 | 0 |
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the FDI Lab - SciCrunch.org Resources search. From here you can search through a compilation of resources used by FDI Lab - SciCrunch.org and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that FDI Lab - SciCrunch.org has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on FDI Lab - SciCrunch.org then you can log in from here to get additional features in FDI Lab - SciCrunch.org such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into FDI Lab - SciCrunch.org you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.