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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pCRZeroT
 
Resource Report
Resource Website
RRID:Addgene_120276 Other PMID:31015513 Vector Backbone:pUC18; Vector Types:Other; Bacterial Resistance:Other 2022-04-22 03:20:37 0
pLD1 translation factors
 
Resource Report
Resource Website
RRID:Addgene_117760 Translation factor cistrons 1-13 Other Other PMID:28977654 The plasmid can be prone to recombination. The depositing lab suggests selecting multiple clones for restriction digest analysis as described in the publication before proceeding. Backbone Size:5018; Vector Backbone:BioBrick pET-24a(+); Vector Types:Other, Escherichia coli; Bacterial Resistance:Other 2022-04-22 03:19:40 0
R6K_tac_mCherry_ChInt
 
Resource Report
Resource Website
RRID:Addgene_187382 mCherry Synthetic Other PMID: This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration). Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2022-09-08 01:02:33 0
pKD4_mCherry
 
Resource Report
Resource Website
RRID:Addgene_187386 mCherry Synthetic Other PMID: This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration). Vector Backbone:pkD4; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2022-09-10 01:02:34 0
pOD_009
 
Resource Report
Resource Website
RRID:Addgene_159520 Other PMID:35377874 - The destination strain should be recA+ and not be able to maintain R6K plasmid - Zeocin is used to select colonies with the integrated vector. - The rhaBAD and lac promoters can be induced with 0.2% rhamnose and 0.5mM IPTG Backbone Marker:Ilya B. Tikh and James C. Samuelson1; Backbone Size:4282; Vector Backbone:pDEL; Vector Types:Synthetic Biology; Bacterial Resistance:Other 2022-10-07 04:51:26 0
S1030
 
Resource Report
Resource Website
RRID:Addgene_105063 none Other PMID:24487694 S1030 Genotype: F’ proA+B+ Δ(lacIZY) zzf::Tn10 lacIQ1 PN25-tetR luxCDE / endA1 recA1 galE15 galK16 nupG rpsL ΔlacIZYA araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) proBA::pir116 araE201 ΔrpoZ Δflu ΔcsgABCDEFG ΔpgaC λ– Vector Backbone:none; Vector Types:; Bacterial Resistance:Other 2022-04-22 03:15:53 0
pF2-DTA-Rosa26
 
Resource Report
Resource Website
RRID:Addgene_42522 Rosa26 genomic fragment Mus musculus Other PMID:23437314 Vector Backbone:pF2; Vector Types:Mouse Targeting; Bacterial Resistance:Other 2022-04-22 03:40:45 0
pBMTB-6
 
Resource Report
Resource Website
RRID:Addgene_22823 Other PMID:16496398 Addgene's QC sequence shows a 1bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity. Backbone Size:7044; Vector Backbone:pBMTB-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2022-04-22 03:36:08 0
pBMT-6
 
Resource Report
Resource Website
RRID:Addgene_22835 Other PMID:16496398 Backbone Size:5862; Vector Backbone:pBMT-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2022-04-22 03:36:09 0
pT2ST
 
Resource Report
Resource Website
RRID:Addgene_59384 TT-ISceI-dfrA Synthetic Other PMID:25255806 iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The trimethoprim resistance gene was taken from EZ-Tn5™ (Epicentre). We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script. Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:Other, template; Bacterial Resistance:Other 2022-04-22 03:44:58 0
pRL1058
 
Resource Report
Resource Website
RRID:Addgene_70686 Transposon Tn5-1058 Other PMID:11607193 Tn5 derivative Tn5-1058 (in plasmid pRL1058) contains a p15A oriV, and its antibiotic-resistance determinants are driven by an Amaranthus hybridus ribulose bisphosphate carboxylase promoter (see pRL439 is this series of plasmids), providing large numbers of transposon mutants. The XbaI site near the L end of Tn5 is the sole unmethylated XbaI in the construct, allowing the placement of luciferases (e.g., Vibrio fischeri luxAB as in pRL1063a). An oriT for conjugal transfer is positioned between the L and R ends of the Tn. Vector Backbone:Transposon Tn5, variously modified; Vector Types:; Bacterial Resistance:Other 2022-04-22 03:47:51 0
pAUC40
 
Resource Report
Resource Website
RRID:Addgene_71298 Other PMID:19843225 Please note that there are several discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function. Backbone Marker:Kaniga, et al Gene. 1991 109: 137-141.; Backbone Size:8509; Vector Backbone:pKNG101; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2022-04-22 03:48:00 0
MIP 227 CoMiP 4in1 wo shRNA p53
 
Resource Report
Resource Website
RRID:Addgene_63727 Mini-intronic-plasmid intron Homo sapiens Other PMID:25628230 In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514. Backbone Size:7608; Vector Backbone:codon-optimized minicircle (CoMiC) construct; Vector Types:Mammalian Expression; Bacterial Resistance:Other codon-optimised 2022-04-22 03:46:13 0
FW102 OL2-62
 
Resource Report
Resource Website
RRID:Addgene_53735 none Other Other PMID:9121589 FW102 containing an F' Kan bearing the placOL2–62-lacZ fusion where the lambda-CI operator (OL2) is centered at position –62 relative to the start of transcription. This is the reporter strain used with the Hochschild lab bacterial 2-hybrid system, as described in the following article: http://www.addgene.org/browse/article/8393/ This strain is also described in Deighan, P., Diez, C.M., Leibman, M., Hochschild, A., and Nickels, B.E. (2008) The bacteriophage λ Q antiterminator protein contacts the β-flap domain of RNA polymerase, PNAS 105: 15305-15310 Vector Backbone:none; Vector Types:; Bacterial Resistance:Other 2022-04-22 03:43:35 0
pEx-insertion-erm-T1T2
 
Resource Report
Resource Website
RRID:Addgene_172212 Other PMID:34676563 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at help@addgene.org or contact our distributors if you have any questions. Backbone Marker:Koropatkin, N.M. (PMID: 18611383); Backbone Size:4172; Vector Backbone:pExchange-tdk; Vector Types:Other, Genetic insertion in Prevotella copri; Bacterial Resistance:Other 2023-05-05 01:04:34 0
pFLAG_OriM
 
Resource Report
Resource Website
RRID:Addgene_110097 Other PMID:29934571 Vector Backbone:pFLAG; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2023-05-10 01:00:33 0
pNR-DEST
 
Resource Report
Resource Website
RRID:Addgene_90101 Other PMID: Backbone Size:10902; Vector Backbone:pPtPBR1; Vector Types:Other, Gateway Destination vector for protein expression in diatoms; Bacterial Resistance:Other 2023-05-10 01:06:39 0
p49202-DEST
 
Resource Report
Resource Website
RRID:Addgene_90100 Other PMID: Backbone Size:11457; Vector Backbone:pPtPBR1; Vector Types:Other, Gateway Destination vector for protein expression in diatoms; Bacterial Resistance:Other 2023-05-10 01:06:39 0
pH4-DEST
 
Resource Report
Resource Website
RRID:Addgene_90099 Other PMID: Backbone Size:10591; Vector Backbone:pPtPBR1; Vector Types:Other, Gateway Destination vector for protein expression in diatoms; Bacterial Resistance:Other 2023-05-10 01:06:39 0
MIP 339 CoMiP coLin28 2A Nanog IRES eGFP
 
Resource Report
Resource Website
RRID:Addgene_63729 Lin28 Homo sapiens Other PMID:25628230 In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514. Vector Backbone:no backbone--uses MiP intron for replication in bacteria; Vector Types:Mammalian Expression; Bacterial Resistance:Other codon optimized 2023-09-15 01:12:40 0

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