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http://www.addgene.org/141095

Species: Other
Genetic Insert: Cas9 and tracrRNA
Vector Backbone Description: Vector Backbone:colE1, pAM beta; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_141095 Copy   

•   Source: Addgene

http://www.addgene.org/135656

Species: Synthetic
Genetic Insert: Intron containing lox site
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pAT19; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Erythromycin
References:
Comments: Use in combination with pQlox66F (addgene 135657) or pQlox 66R (addgene 135658), then pQcre1 (addgene 135659). Transcription of LtrB-derived group II intron is driven by the pFer promoter. Redesign intron to target gene using TargeTron or Clostron algorithms (links below), then clone by SOE-PCR or gene synthesis ligation between NdeI and BsrGI sites. Lox66 and Lox71 must be in same orientation to delete the intervening sequence, but the directions of introns relative to one another can generate inverted repeats in the chromosome after deletion has taken place, leading to futher recombinations events. See publication for details. Intron redesign links http://www.clostron.com/clostron1.php http://www.targetrons.com/targetron_pLtrB.php Reference for intron redesign Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP. The ClosTron: A universal gene knock-out system for the genus Clostridium. J Microbiol Methods. 2007;70(3):452-464. Perutka J, Wang W, Goerlitz D, Lambowitz AM. Use of Computer-designed Group II Introns to Disrupt Escherichia coli DExH / D-box Protein and DNA Helicase Genes. 2004:421-439.

Proper citation: RRID:Addgene_135656 Copy   

•   Source: Addgene

http://www.addgene.org/135659

Species: Other
Genetic Insert: Cre recombinase
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pAT19; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments: Cre protein under the control of the gram + PpagA promoter. Leibig M, Krismer B, Kolb M, Friede A, Götz F, Bertram R. 2008. Marker removal in staphylococci via Cre recombinase and different lox sites. Appl Environ Microbiol 74:1316– 1323.

Proper citation: RRID:Addgene_135659 Copy   

•   Source: Addgene

http://www.addgene.org/135662

Species: Synthetic
Genetic Insert: Lox511/66 and LoxFas/71
Vector Backbone Description: Vector Backbone:pAT19; Vector Types:Bacterial Expression, Other, Clostridial vector, pAMβ1 origin; Bacterial Resistance:Erythromycin
References:
Comments: Cargo can be inserted between the lox sites using the SpeI and XhoI sites.

Proper citation: RRID:Addgene_135662 Copy   

•   Source: Addgene

http://www.addgene.org/177208

Species: Other
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pmr101A; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments: Braatsch S, Helmark S, Kranz H, Koebmann B, Jensen PR. Escherichia coli strains with promoter libraries constructed by Red/ET recombination pave the way for transcriptional fine-tuning. Biotechniques. 2008 Sep;45(3):335-7. doi: 10.2144/000112907. PMID: 18778259.

Proper citation: RRID:Addgene_177208 Copy   

•   Source: Addgene

http://www.addgene.org/177206

Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pmr101A; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments: Braatsch S, Helmark S, Kranz H, Koebmann B, Jensen PR. Escherichia coli strains with promoter libraries constructed by Red/ET recombination pave the way for transcriptional fine-tuning. Biotechniques. 2008 Sep;45(3):335-7. doi: 10.2144/000112907. PMID: 18778259.

Proper citation: RRID:Addgene_177206 Copy   

•   Source: Addgene

http://www.addgene.org/25819

Species:
Genetic Insert: qQint
Vector Backbone Description: Backbone Size:6559; Vector Backbone:pAT19; Vector Types:Other, bacterial gene inactivation; Bacterial Resistance:Erythromycin
References:
Comments: Between Addgene sequence and author sequence, there is a 12 nucleotide mismatched region. It is in the intron sequence, which needs to be replaced in order to use the plasmid and should not be of concern.

Proper citation: RRID:Addgene_25819 Copy   

•   Source: Addgene

http://www.addgene.org/90197

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Philippe Moreillon (PMID 10816506); Backbone Size:6000; Vector Backbone:pAMB1 (pORI23); Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments: This plasmid can also be used for His-tag or Strep-tag fusion protein expression in gram-positive bacteria.

Proper citation: RRID:Addgene_90197 Copy   

•   Source: Addgene

http://www.addgene.org/90194

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Philippe Moreillon (PMID 10816506); Backbone Size:6000; Vector Backbone:pAMB1 (pORI23); Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_90194 Copy   

•   Source: Addgene

http://www.addgene.org/90196

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Philippe Moreillon (PMID 10816506); Backbone Size:6000; Vector Backbone:pAMB1 (pORI23); Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_90196 Copy   

•   Source: Addgene

http://www.addgene.org/71312

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4963; Vector Backbone:pIL253; Vector Types:Bacterial Expression, Other, E. coli-gram positive bacteria shuttle vector; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_71312 Copy   

•   Source: Addgene

http://www.addgene.org/71803

Species: Other
Genetic Insert: gusA3
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:4568; Vector Backbone:pTRK882; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_71803 Copy   

•   Source: Addgene

http://www.addgene.org/71595

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2200; Vector Backbone:pWV01; Vector Types:Other, integration vector for bacteria; Bacterial Resistance:Erythromycin
References:
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at help@addgene.org or contact our distributors if you have any questions. This plasmid has been found to be somewhat prone to multimerization. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.

Proper citation: RRID:Addgene_71595 Copy   

•   Source: Addgene

http://www.addgene.org/191001

Species: Other
Genetic Insert: red chromoprotein
Vector Backbone Description: Backbone Size:7023; Vector Backbone:pBTK402; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Erythromycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.02.15.528778v1 for bioRxiv preprint.

Proper citation: RRID:Addgene_191001 Copy   

•   Source: Addgene

http://www.addgene.org/199563

Species: Other
Genetic Insert: Fncas12a
Vector Backbone Description: Backbone Size:4200; Vector Backbone:pGG3221; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology, Other, Tetracycline Inducible; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_199563 Copy   

•   Source: Addgene

http://www.addgene.org/203682

Species:
Genetic Insert: Yellow Fluorescent Protein (YFP)
Vector Backbone Description: Vector Backbone:pNF8; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments: Optimized version of the Cyan- and Yellow fluorescent proteins were created as follows: The hsp60 promoter driven GFP+ [19] was amplified and cloned into the pUC18 plasmid. pUC-yfp++ was obtained by introducing the following mutations by site-directed mutagenesis (Stratagene): F46L, S65G, V68A, S72A, S175G, T203Y, whereas the pUC-cfp++, was obtained by introducing the K26R, Y66W, N146I, N164H, S175G, N212K mutations

Proper citation: RRID:Addgene_203682 Copy   

•   Source: Addgene

http://www.addgene.org/203681

Species:
Genetic Insert: Cyan Fluorescent Protein (CFP)
Vector Backbone Description: Vector Backbone:pNF8; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments: Please note that this plasmid contains an additional IS4-like element in the backbone.

Proper citation: RRID:Addgene_203681 Copy   

•   Source: Addgene

http://www.addgene.org/208735

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4475; Vector Backbone:pSR1002; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Erythromycin
References:
Comments: Erythromycin resistance in S. aureus

Proper citation: RRID:Addgene_208735 Copy   

•   Source: Addgene

http://www.addgene.org/153516

Species: Other
Genetic Insert: dCas9Str
Vector Backbone Description: Backbone Marker:Gary Dunny; Backbone Size:8514; Vector Backbone:pMSP3545; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_153516 Copy   

•   Source: Addgene

http://www.addgene.org/191348

Species: Other
Genetic Insert: Plac-RFP (IPTG-inducible red fluorescent protein)
Vector Backbone Description: Backbone Marker:Chain Biotech; Backbone Size:3823; Vector Backbone:pMTL83241; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_191348 Copy   

•   Source: Addgene