Searching the RRID Resource Information Network
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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pCB578 Resource Report Resource Website |
RRID:Addgene_141095 | Cas9 and tracrRNA | Other | Erythromycin | PMID:30156038 | Vector Backbone:colE1, pAM beta; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2023-04-01 01:02:43 | 0 | ||
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pQlox71R Resource Report Resource Website |
RRID:Addgene_135656 | Intron containing lox site | Synthetic | Erythromycin | PMID:31826971 | Use in combination with pQlox66F (addgene 135657) or pQlox 66R (addgene 135658), then pQcre1 (addgene 135659). Transcription of LtrB-derived group II intron is driven by the pFer promoter. Redesign intron to target gene using TargeTron or Clostron algorithms (links below), then clone by SOE-PCR or gene synthesis ligation between NdeI and BsrGI sites. Lox66 and Lox71 must be in same orientation to delete the intervening sequence, but the directions of introns relative to one another can generate inverted repeats in the chromosome after deletion has taken place, leading to futher recombinations events. See publication for details. Intron redesign links http://www.clostron.com/clostron1.php http://www.targetrons.com/targetron_pLtrB.php Reference for intron redesign Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP. The ClosTron: A universal gene knock-out system for the genus Clostridium. J Microbiol Methods. 2007;70(3):452-464. Perutka J, Wang W, Goerlitz D, Lambowitz AM. Use of Computer-designed Group II Introns to Disrupt Escherichia coli DExH / D-box Protein and DNA Helicase Genes. 2004:421-439. | Backbone Size:6500; Vector Backbone:pAT19; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Erythromycin | 2023-04-01 01:02:24 | 0 | |
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pQcre1 Resource Report Resource Website |
RRID:Addgene_135659 | Cre recombinase | Other | Erythromycin | PMID:31826971 | Cre protein under the control of the gram + PpagA promoter. Leibig M, Krismer B, Kolb M, Friede A, Götz F, Bertram R. 2008. Marker removal in staphylococci via Cre recombinase and different lox sites. Appl Environ Microbiol 74:1316– 1323. | Backbone Size:6500; Vector Backbone:pAT19; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2023-04-01 01:02:24 | 0 | |
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pQadd2 Resource Report Resource Website |
RRID:Addgene_135662 | Lox511/66 and LoxFas/71 | Synthetic | Erythromycin | PMID:31826971 | Cargo can be inserted between the lox sites using the SpeI and XhoI sites. | Vector Backbone:pAT19; Vector Types:Bacterial Expression, Other, Clostridial vector, pAMβ1 origin; Bacterial Resistance:Erythromycin | 2023-04-01 01:02:24 | 0 | |
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pmr101A-cPr-mNeonGreen (ErmR) Resource Report Resource Website |
RRID:Addgene_177208 | mNeonGreen | Other | Erythromycin | PMID: | Braatsch S, Helmark S, Kranz H, Koebmann B, Jensen PR. Escherichia coli strains with promoter libraries constructed by Red/ET recombination pave the way for transcriptional fine-tuning. Biotechniques. 2008 Sep;45(3):335-7. doi: 10.2144/000112907. PMID: 18778259. | Vector Backbone:pmr101A; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | Yeast codon optimized | 2023-04-01 01:04:42 | 0 |
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pmr101A-cPr-mCherry (ErmR) Resource Report Resource Website |
RRID:Addgene_177206 | mCherry | Other | Erythromycin | PMID: | Braatsch S, Helmark S, Kranz H, Koebmann B, Jensen PR. Escherichia coli strains with promoter libraries constructed by Red/ET recombination pave the way for transcriptional fine-tuning. Biotechniques. 2008 Sep;45(3):335-7. doi: 10.2144/000112907. PMID: 18778259. | Vector Backbone:pmr101A; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2023-04-01 01:04:42 | 0 | |
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pQint Resource Report Resource Website |
RRID:Addgene_25819 | qQint | Erythromycin | PMID:19775243 | Between Addgene sequence and author sequence, there is a 12 nucleotide mismatched region. It is in the intron sequence, which needs to be replaced in order to use the plasmid and should not be of concern. | Backbone Size:6559; Vector Backbone:pAT19; Vector Types:Other, bacterial gene inactivation; Bacterial Resistance:Erythromycin | plasmid to make targeted insertions in Clostridium phytofermentans chromosome using group II intron | 2023-04-01 01:05:42 | 0 | |
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pIB190 Resource Report Resource Website |
RRID:Addgene_90197 | Erythromycin | PMID:18667560 | This plasmid can also be used for His-tag or Strep-tag fusion protein expression in gram-positive bacteria. | Backbone Marker:Philippe Moreillon (PMID 10816506); Backbone Size:6000; Vector Backbone:pAMB1 (pORI23); Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2023-04-01 01:09:00 | 0 | |||
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pIB184 (EM) Resource Report Resource Website |
RRID:Addgene_90194 | Erythromycin | PMID:18667560 | Backbone Marker:Philippe Moreillon (PMID 10816506); Backbone Size:6000; Vector Backbone:pAMB1 (pORI23); Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2023-04-01 01:09:00 | 0 | ||||
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pIB185 Resource Report Resource Website |
RRID:Addgene_90196 | Erythromycin | PMID:18667560 | Backbone Marker:Philippe Moreillon (PMID 10816506); Backbone Size:6000; Vector Backbone:pAMB1 (pORI23); Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2023-04-01 01:09:00 | 0 | ||||
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pTRKH2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_71312 | Erythromycin | PMID:8299952 | Backbone Size:4963; Vector Backbone:pIL253; Vector Types:Bacterial Expression, Other, E. coli-gram positive bacteria shuttle vector; Bacterial Resistance:Erythromycin | 2023-04-01 01:07:55 | 1 | ||||
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pTRK892 Resource Report Resource Website |
RRID:Addgene_71803 | gusA3 | Other | Erythromycin | PMID:21375708 | Backbone Marker:N/A; Backbone Size:4568; Vector Backbone:pTRK882; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | D573A | 2023-04-01 01:07:57 | 0 | |
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pORI28 Resource Report Resource Website |
RRID:Addgene_71595 | Erythromycin | PMID:9003306 | Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at help@addgene.org or contact our distributors if you have any questions. This plasmid has been found to be somewhat prone to multimerization. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid. | Backbone Size:2200; Vector Backbone:pWV01; Vector Types:Other, integration vector for bacteria; Bacterial Resistance:Erythromycin | 2023-04-01 01:07:56 | 0 | |||
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pBTK1006 (pSL5) Resource Report Resource Website |
RRID:Addgene_191001 | red chromoprotein | Other | Erythromycin | PMID:37225918 | Please visit https://www.biorxiv.org/content/10.1101/2023.02.15.528778v1 for bioRxiv preprint. | Backbone Size:7023; Vector Backbone:pBTK402; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Erythromycin | 2023-07-19 01:04:10 | 0 | |
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pPIPL12-FnCas12a Resource Report Resource Website |
RRID:Addgene_199563 | Fncas12a | Other | Erythromycin | PMID:37947521 | Backbone Size:4200; Vector Backbone:pGG3221; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology, Other, Tetracycline Inducible; Bacterial Resistance:Erythromycin | 2023-11-15 12:06:30 | 0 | ||
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PJEBAN3 Resource Report Resource Website |
RRID:Addgene_203682 | Yellow Fluorescent Protein (YFP) | Erythromycin | PMID:17014739 | Optimized version of the Cyan- and Yellow fluorescent proteins were created as follows: The hsp60 promoter driven GFP+ [19] was amplified and cloned into the pUC18 plasmid. pUC-yfp++ was obtained by introducing the following mutations by site-directed mutagenesis (Stratagene): F46L, S65G, V68A, S72A, S175G, T203Y, whereas the pUC-cfp++, was obtained by introducing the K26R, Y66W, N146I, N164H, S175G, N212K mutations | Vector Backbone:pNF8; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2023-09-15 01:09:46 | 0 | ||
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PJEBAN2 Resource Report Resource Website |
RRID:Addgene_203681 | Cyan Fluorescent Protein (CFP) | Erythromycin | PMID:17014739 | Please note that this plasmid contains an additional IS4-like element in the backbone. | Vector Backbone:pNF8; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2023-09-22 01:05:01 | 0 | ||
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pSR1002 Resource Report Resource Website |
RRID:Addgene_208735 | Erythromycin | PMID:38064657 | Erythromycin resistance in S. aureus | Backbone Size:4475; Vector Backbone:pSR1002; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Erythromycin | 2023-12-16 12:05:13 | 0 | |||
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pMSP3545-dCas9Str Resource Report Resource Website |
RRID:Addgene_153516 | dCas9Str | Other | Erythromycin | PMID:33082254 | Backbone Marker:Gary Dunny; Backbone Size:8514; Vector Backbone:pMSP3545; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2024-03-01 12:02:55 | 0 | ||
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pQmod3E-GG Resource Report Resource Website |
RRID:Addgene_191348 | Plac-RFP (IPTG-inducible red fluorescent protein) | Other | Erythromycin | PMID:36427328 | Backbone Marker:Chain Biotech; Backbone Size:3823; Vector Backbone:pMTL83241; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2024-03-19 01:04:49 | 0 |
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