Searching the RRID Resource Information Network
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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qi5 Resource Report Resource Website |
RRID:Addgene_24501 | Gq alpha | Mus musculus | Ampicillin and Tetracycline | PMID:8387644 | This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gi alpha residues (EYNLV to DCGLF). This construct allows many Gi-coupled receptors to stimulate phospholipase C (PLC). This is the most popular chimera, perhaps because it is easier to talk about coupling to Gi-coupled receptors with "qi5" rather than "qo5" or "qz5." Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. | Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | Gq alpha with the C-terminal amino acids changed from Gq alpha to Gi alpha residues (EYNLV to DCGLF) | 2022-09-09 01:02:49 | 0 |
|
qo5 Resource Report Resource Website |
RRID:Addgene_24500 | Gq alpha | Mus musculus | Ampicillin and Tetracycline | PMID:8387644 | This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues (EYNLV to GCGLY). Works the same as qi5 but (for unknown reasons) has a slightly lower basal PLC activity. This can increase the signal-to-noise ratio. Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. | Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | Gq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues (EYNLV to GCGLY) | 2022-09-09 01:02:49 | 0 |
|
qs5 Resource Report Resource Website |
RRID:Addgene_24498 | Gq alpha | Mus musculus | Ampicillin and Tetracycline | PMID:8863834 | This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gs alpha residues (EYNLV to QYELL). This construct allows some Gs-coupled receptors to stimulate phospholipase C. Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. | Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | Replacement of the five carboxyl-terminal amino acids of Gq alpha (EYNLV) with those of Gs alpha(QYELL) | 2022-09-09 01:02:49 | 0 |
|
pCDM8 hCD81 Resource Report Resource Website 1+ mentions |
RRID:Addgene_11588 | CD81 | Homo sapiens | Ampicillin and Tetracycline | PMID:1695320 | Backbone Marker:Invitrogen; Backbone Size:4400; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | 2022-04-22 03:19:12 | 1 | ||
|
pcDNA1-Tastin Resource Report Resource Website |
RRID:Addgene_11871 | Tastin | Homo sapiens | Ampicillin and Tetracycline | PMID:7758945 | Backbone Size:4033; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | 2022-04-22 03:19:59 | 0 | ||
|
pJH548 Resource Report Resource Website |
RRID:Addgene_13328 | RAG-1 | Mus musculus | Ampicillin and Tetracycline | PMID:8284210 | cDNA was synthesized by reverse transcription of total RNA obtained from cell line 22D6, an Abelson murine leukemia virus transformed pre-B cell line. RAG-1 cDNA was then amplified by PCR, using specific primers designed also to add restriction enzyme recognition sites adjacent to the largest open reading frame for subsequent cloning. PCR products were subcloned into the pCDM8 vector. | Backbone Marker:Invitrogen; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | 2022-04-22 03:24:48 | 0 | |
|
pJH549 Resource Report Resource Website |
RRID:Addgene_13329 | RAG-2 | Mus musculus | Ampicillin and Tetracycline | PMID:8284210 | cDNA was synthesized by reverse transcription of total RNA obtained from cell line 22D6, an Abelson murine leukemia virus transformed pre-B cell line. RAG-2 cDNA was then amplified by PCR, using specific primers designed also to add restriction enzyme recognition sites adjacent to the largest open reading frame for subsequent cloning. PCR products were subcloned into the pCDM8 vector. | Backbone Marker:Invitrogen; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | 2022-04-22 03:24:48 | 0 | |
|
integrin beta6 pcDNA1 neo Resource Report Resource Website |
RRID:Addgene_13580 | integrin beta 6 | Homo sapiens | Ampicillin and Tetracycline | PMID:8120056 | Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | 2022-04-22 03:25:22 | 0 | ||
|
integrin alpha9 W999A pcDNA1 neo Resource Report Resource Website |
RRID:Addgene_13589 | integrin alpha 9 W999A | Homo sapiens | Ampicillin and Tetracycline | PMID:11598204 | See article for sequences of the primers used for site-directed mutagenesis. | Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | W999A | 2022-04-22 03:25:24 | 0 |
|
integrin alpha9 / alpha4 pcDNA1 neo Resource Report Resource Website |
RRID:Addgene_13587 | integrin alpha 9 / alpha 4 | Homo sapiens | Ampicillin and Tetracycline | PMID:11598204 | See article for cloning details. | Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | Integrin alpha 9 chimera containing the cytoplasmic domain of integrin alpha 4 | 2022-04-22 03:25:24 | 0 |
|
integrin alpha9 / alpha2 pcDNA1 neo Resource Report Resource Website |
RRID:Addgene_13586 | integrin alpha 9 / alpha 2 | Homo sapiens | Ampicillin and Tetracycline | PMID:11598204 | See article for cloning details. | Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | Integrin alpha 9 chimera containing the cytoplasmic domain of integrin alpha 2 | 2022-04-22 03:25:24 | 0 |
|
pTpPBR11_accNAT Resource Report Resource Website |
RRID:Addgene_154034 | Ampicillin and Tetracycline | PMID: | selectable marker added for centric diatoms | Vector Backbone:pBR322; Vector Types:Other, Diatom cloning; Bacterial Resistance:Ampicillin and Tetracycline | 2022-04-22 03:28:40 | 0 | |||
|
pRS_2N_400 Resource Report Resource Website |
RRID:Addgene_170479 | T7A1, O2, O-, Lambda t1 in lacZ fragment | Synthetic | Ampicillin and Tetracycline | PMID: | LacI operators separated by 400 base pairs. | Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline | 2022-04-22 03:32:30 | 0 | |
|
CHOP 6: mCHOP-WT-9E10-pcDNA1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_21913 | CHOP10 | Mus musculus | Ampicillin and Tetracycline | PMID: | From depositing lab: "9E10 (Myc-epitope) tagged mouse CHOP coding region only. The insert was joined to the epitope tag using patch-PCR and incorporating unique BamH1 and Xho1 sites for ligation into pcDNA1. Stocks are provided as MC1060 (or MC1061, we are not sure) host strain transformed with the plasmid. Note that pCDNA1 plasmids must be propagated on combined amp and tet selection. I would recommedn that you consider transferring the insert into a better host strain such as pcDNA3" | Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | 2022-04-22 03:35:54 | 2 | |
|
sq5 Resource Report Resource Website |
RRID:Addgene_24499 | Gs alpha | Rattus norvegicus | Ampicillin and Tetracycline | PMID:8863834 | This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues (QYELL to EYNLV). This construct allows some Gq-coupled receptors to stimulate Adenylate cyclase. There isn't much experience with this chimera at the moment. It may be useful for people who find the AC stimulation is better readout of receptor activation than PLC stimulation. Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. | Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues (QYELL to EYNLV). | 2022-04-22 03:36:41 | 0 |
|
pAXEF-Rap1-E63(GTP) Resource Report Resource Website 1+ mentions |
RRID:Addgene_32698 | RAP1 | Homo sapiens | Ampicillin and Tetracycline | PMID:15469846 | Q63 changed to E to give phenotype | Vector Backbone:pAXEF-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | E63: Consitutively GTP-bound | 2022-04-22 03:38:28 | 1 |
|
pRS_SN_400 Resource Report Resource Website |
RRID:Addgene_170472 | T7A1, Os, O-, Lambda t1 in lacZ fragment | Synthetic | Ampicillin and Tetracycline | PMID: | LacI operators separated by 400 base pairs. | Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline | 2022-04-22 03:32:29 | 0 | |
|
pYY_S1_400 Resource Report Resource Website |
RRID:Addgene_170470 | T7A1, Os, O1, lambda t1, in lacZ fragment | Synthetic | Ampicillin and Tetracycline | PMID:28940077 | LacI operators separated by 400 base pairs. | Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline | 2022-04-22 03:32:29 | 0 | |
|
pPtPBR2 Resource Report Resource Website |
RRID:Addgene_80387 | Ampicillin and Tetracycline | PMID: | Backbone Size:4360; Vector Backbone:pBR322; Vector Types:Synthetic Biology, Other, ; Episomal vector for Phaeodactylum tricornutum; Bacterial Resistance:Ampicillin and Tetracycline | 2022-04-22 03:50:32 | 0 | ||||
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pPtPBR11 Resource Report Resource Website |
RRID:Addgene_80386 | Ampicillin and Tetracycline | PMID: | Sequence is associated with the manuscript Diner et al. 2016 in review | Backbone Size:4360; Vector Backbone:pBR322; Vector Types:Synthetic Biology, Other, : Episomal vector for Phaeodactylum tricornutum; Bacterial Resistance:Ampicillin and Tetracycline | 2022-04-22 03:50:32 | 0 |
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