Plasmids   Select Another Resource Report Type

http://www.addgene.org/102939

Species: Other
Genetic Insert: mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
Vector Backbone Description: Vector Backbone:pSAM; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: The Plac promoter from pGFP-Mut3.1 (Clonetech), along with its associated ribosome binding sequence, was amplified via PCR. Engineered 59 BamHI and 39 NdeI restriction sites were used to sub-clone the resulting fragment into a BamHI/NdeI (New England Biolabs) double digested pSAM_Bt vector upstream of the himar1C9 transposase gene. The kanamycin resistance gene from pKD4 was amplified and ligated using the restriction sites MfeI and XbaI, replacing the erythromycin resistance gene ermG in pSAM_Bt. The resulting transposon mutagenesis vector, pSAM-Ec, was stored and propagated in the pir+ E. coli strain EcS17.

Proper citation: RRID:Addgene_102939 Copy   

•   Source: Addgene

http://www.addgene.org/103191

Species: Homo sapiens
Genetic Insert: hsa-miR-125b-5p target
Vector Backbone Description: Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC.

Proper citation: RRID:Addgene_103191 Copy   

•   Source: Addgene

http://www.addgene.org/103156

Species: Homo sapiens
Genetic Insert: hsa-let-7f-5p target
Vector Backbone Description: Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC.

Proper citation: RRID:Addgene_103156 Copy   

•   Source: Addgene

http://www.addgene.org/103165

Species: Homo sapiens
Genetic Insert: hsa-miR-101-3p target
Vector Backbone Description: Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC.

Proper citation: RRID:Addgene_103165 Copy   

•   Source: Addgene

http://www.addgene.org/103169

Species: Homo sapiens
Genetic Insert: hsa-miR-105-3p target
Vector Backbone Description: Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC.

Proper citation: RRID:Addgene_103169 Copy   

•   Source: Addgene

http://www.addgene.org/103146

Species: Homo sapiens
Genetic Insert: hsa-let-7a-5p target
Vector Backbone Description: Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC.

Proper citation: RRID:Addgene_103146 Copy   

•   Source: Addgene

http://www.addgene.org/1248

Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJC45S; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_1248 Copy   

•   Source: Addgene

http://www.addgene.org/124810

Species: Rattus norvegicus
Genetic Insert: rat IgG2a Hinge-G4S-Sortag-Histag
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:3956; Vector Backbone:pCR4 TOPO TA; Vector Types:Bacterial Expression, CRISPR, Other, HDR template; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_124810 Copy   

•   Source: Addgene

http://www.addgene.org/121902

Species: Danio rerio
Genetic Insert: ApoA4a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCRII; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_121902 Copy   

•   Source: Addgene

http://www.addgene.org/121897

Species: Danio rerio
Genetic Insert: ApoBa
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCRII; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_121897 Copy   

•   Source: Addgene

http://www.addgene.org/121898

Species: Danio rerio
Genetic Insert: ApoBb.1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCRII; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_121898 Copy   

•   Source: Addgene

http://www.addgene.org/121184

Species: Synthetic
Genetic Insert: CMV-OsTIR1-loxP-PURO-loxP
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: This plasmid is a variant of Addgene plasmid 72834.

Proper citation: RRID:Addgene_121184 Copy   

•   Source: Addgene

http://www.addgene.org/126577

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR2.1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_126577 Copy   

•   Source: Addgene

http://www.addgene.org/53969

Species: Mus musculus
Genetic Insert: mouse Ins2 gene partial
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pCR2.1-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_53969 Copy   

•   Source: Addgene

http://www.addgene.org/111873

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5127; Vector Backbone:pONSY; Vector Types:Other, Capsaspora owczarzaki; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_111873 Copy   

•   Source: Addgene

http://www.addgene.org/111876

Species:
Genetic Insert: Lifeact fused to mCherry
Vector Backbone Description: Backbone Size:5127; Vector Backbone:pONSY; Vector Types:Other, Capsaspora owczarzaki; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_111876 Copy   

•   Source: Addgene

http://www.addgene.org/111874

Species:
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5127; Vector Backbone:pONSY; Vector Types:Other, Capsaspora owczarzaki; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_111874 Copy   

•   Source: Addgene

http://www.addgene.org/111878

Species: Other
Genetic Insert: Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
Vector Backbone Description: Backbone Size:5127; Vector Backbone:pONSY; Vector Types:Other, Capsaspora owczarzaki; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_111878 Copy   

•   Source: Addgene

http://www.addgene.org/117480

Species: Other
Genetic Insert: uracil phosphoribosyltransferase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCR4/TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Selection for kanamycin resistance after introduction of pMO746 identifies those cells in which integration into the chromosome of part or all of the plasmid has occurred. Kanamycin resistance is a strong selection in the sulfate-reducing bacteria (SRB). Ampicillin is not universally effective in the SRB but is often used for selection in Escherichia coli. Thus this plasmid can be readily grown in E. coli but is actually unstable in the SRB.

Proper citation: RRID:Addgene_117480 Copy   

•   Source: Addgene

http://www.addgene.org/119559

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pPMQAK1; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.

Proper citation: RRID:Addgene_119559 Copy   

•   Source: Addgene