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http://www.addgene.org/120276

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Other; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_120276 Copy   

•   Source: Addgene

http://www.addgene.org/117760

Species: Other
Genetic Insert: Translation factor cistrons 1-13
Vector Backbone Description: Backbone Size:5018; Vector Backbone:BioBrick pET-24a(+); Vector Types:Other, Escherichia coli; Bacterial Resistance:Other
References:
Comments: The plasmid can be prone to recombination. The depositing lab suggests selecting multiple clones for restriction digest analysis as described in the publication before proceeding.

Proper citation: RRID:Addgene_117760 Copy   

•   Source: Addgene

http://www.addgene.org/187382

Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration).

Proper citation: RRID:Addgene_187382 Copy   

•   Source: Addgene

http://www.addgene.org/187386

Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pkD4; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration).

Proper citation: RRID:Addgene_187386 Copy   

•   Source: Addgene

http://www.addgene.org/159520

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ilya B. Tikh and James C. Samuelson1; Backbone Size:4282; Vector Backbone:pDEL; Vector Types:Synthetic Biology; Bacterial Resistance:Other
References:
Comments: - The destination strain should be recA+ and not be able to maintain R6K plasmid - Zeocin is used to select colonies with the integrated vector. - The rhaBAD and lac promoters can be induced with 0.2% rhamnose and 0.5mM IPTG

Proper citation: RRID:Addgene_159520 Copy   

•   Source: Addgene

http://www.addgene.org/105063

Species:
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Other
References:
Comments: S1030 Genotype: F’ proA+B+ Δ(lacIZY) zzf::Tn10 lacIQ1 PN25-tetR luxCDE / endA1 recA1 galE15 galK16 nupG rpsL ΔlacIZYA araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) proBA::pir116 araE201 ΔrpoZ Δflu ΔcsgABCDEFG ΔpgaC λ–

Proper citation: RRID:Addgene_105063 Copy   

•   Source: Addgene

http://www.addgene.org/42522

Species: Mus musculus
Genetic Insert: Rosa26 genomic fragment
Vector Backbone Description: Vector Backbone:pF2; Vector Types:Mouse Targeting; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_42522 Copy   

•   Source: Addgene

http://www.addgene.org/22823

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7044; Vector Backbone:pBMTB-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: Addgene's QC sequence shows a 1bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity.

Proper citation: RRID:Addgene_22823 Copy   

•   Source: Addgene

http://www.addgene.org/22835

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5862; Vector Backbone:pBMT-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_22835 Copy   

•   Source: Addgene

http://www.addgene.org/59384

Species: Synthetic
Genetic Insert: TT-ISceI-dfrA
Vector Backbone Description: Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:Other, template; Bacterial Resistance:Other
References:
Comments: iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The trimethoprim resistance gene was taken from EZ-Tn5™ (Epicentre). We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script.

Proper citation: RRID:Addgene_59384 Copy   

•   Source: Addgene

http://www.addgene.org/70686

Species:
Genetic Insert: Transposon Tn5-1058
Vector Backbone Description: Vector Backbone:Transposon Tn5, variously modified; Vector Types:; Bacterial Resistance:Other
References:
Comments: Tn5 derivative Tn5-1058 (in plasmid pRL1058) contains a p15A oriV, and its antibiotic-resistance determinants are driven by an Amaranthus hybridus ribulose bisphosphate carboxylase promoter (see pRL439 is this series of plasmids), providing large numbers of transposon mutants. The XbaI site near the L end of Tn5 is the sole unmethylated XbaI in the construct, allowing the placement of luciferases (e.g., Vibrio fischeri luxAB as in pRL1063a). An oriT for conjugal transfer is positioned between the L and R ends of the Tn.

Proper citation: RRID:Addgene_70686 Copy   

•   Source: Addgene

http://www.addgene.org/71298

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Kaniga, et al Gene. 1991 109: 137-141.; Backbone Size:8509; Vector Backbone:pKNG101; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: Please note that there are several discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Proper citation: RRID:Addgene_71298 Copy   

•   Source: Addgene

http://www.addgene.org/63727

Species: Homo sapiens
Genetic Insert: Mini-intronic-plasmid intron
Vector Backbone Description: Backbone Size:7608; Vector Backbone:codon-optimized minicircle (CoMiC) construct; Vector Types:Mammalian Expression; Bacterial Resistance:Other
References:
Comments: In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514.

Proper citation: RRID:Addgene_63727 Copy   

•   Source: Addgene

http://www.addgene.org/53735

Species: Other
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Other
References:
Comments: FW102 containing an F' Kan bearing the placOL2–62-lacZ fusion where the lambda-CI operator (OL2) is centered at position –62 relative to the start of transcription. This is the reporter strain used with the Hochschild lab bacterial 2-hybrid system, as described in the following article: http://www.addgene.org/browse/article/8393/ This strain is also described in Deighan, P., Diez, C.M., Leibman, M., Hochschild, A., and Nickels, B.E. (2008) The bacteriophage λ Q antiterminator protein contacts the β-flap domain of RNA polymerase, PNAS 105: 15305-15310

Proper citation: RRID:Addgene_53735 Copy   

•   Source: Addgene

http://www.addgene.org/172212

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Koropatkin, N.M. (PMID: 18611383); Backbone Size:4172; Vector Backbone:pExchange-tdk; Vector Types:Other, Genetic insertion in Prevotella copri; Bacterial Resistance:Other
References:
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at help@addgene.org or contact our distributors if you have any questions.

Proper citation: RRID:Addgene_172212 Copy   

•   Source: Addgene

http://www.addgene.org/110097

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pFLAG; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_110097 Copy   

•   Source: Addgene

http://www.addgene.org/63729

Species: Homo sapiens
Genetic Insert: Lin28
Vector Backbone Description: Vector Backbone:no backbone--uses MiP intron for replication in bacteria; Vector Types:Mammalian Expression; Bacterial Resistance:Other
References:
Comments: In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514.

Proper citation: RRID:Addgene_63729 Copy   

•   Source: Addgene

http://www.addgene.org/110083

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMEXC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_110083 Copy   

•   Source: Addgene

http://www.addgene.org/110077

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMINTC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_110077 Copy   

•   Source: Addgene

http://www.addgene.org/187394

Species: Synthetic
Genetic Insert: GFP, mCherry
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration).

Proper citation: RRID:Addgene_187394 Copy   

•   Source: Addgene