Species: Synthetic
Genetic Insert: cis-aconitate decarboxylase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3519; Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_124842 Copy
Species: Synthetic
Genetic Insert: SPOON
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124840 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-FLPo
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: In the NGS sequence, polymorphisms are present in a GC-rich region of the Chicken beta-actin promoter that do not affect the function of the plasmid.
Proper citation: RRID:Addgene_124834 Copy
Species: Synthetic
Genetic Insert: pac-T2A-FLPo
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124835 Copy
Species: Synthetic
Genetic Insert: pac-T2A-dre
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124833 Copy
Species: Synthetic
Genetic Insert: dsRed
Vector Backbone Description: Backbone Marker:BCM; Backbone Size:24000; Vector Backbone:pD21; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments: As this plasmid is very large, NGS had difficulty sequencing it. There are several differences between Addgene's NGS and depositors sequence. We recommend running additional diagnostic tests before use.
Depositor also recommends growing plasmid at 30 degrees C due to their size.
Proper citation: RRID:Addgene_124977 Copy
Species: Synthetic
Genetic Insert: copGFP
Vector Backbone Description: Backbone Marker:BCM; Backbone Size:24000; Vector Backbone:pD21; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments: As this plasmid is very large, NGS had difficulty sequencing it. There are several differences between Addgene's NGS and depositor's sequence. We recommend running additional diagnostic tests before use.
Depositor also recommends growing plasmid at 30 degrees C due to their size.
Proper citation: RRID:Addgene_124963 Copy
Species: Synthetic
Genetic Insert: sgGTTG
Vector Backbone Description: Vector Backbone:pAAVsc; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121512 Copy
Species: Synthetic
Genetic Insert: Polyadenylation signal (polyA)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121746 Copy
Species: Synthetic
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121740 Copy
Species: Synthetic
Genetic Insert: Firefly Luciferase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments: Luc2 PCR'd from pGL4.23 (Promega)
Proper citation: RRID:Addgene_121741 Copy
Species: Synthetic
Genetic Insert: βgal
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121738 Copy
Species: Synthetic
Genetic Insert: βgal (open)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121739 Copy
Species: Synthetic
Genetic Insert: cTnT promoter (Cardiomyocyte specific)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2557; Vector Backbone:pDONR221 P1-P4; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121690 Copy
Species: Synthetic
Genetic Insert: CMV promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pDONR221 P1-P5r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121692 Copy
Species: Synthetic
Genetic Insert: EF1a promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pDONR221 P1-P5r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121693 Copy
Species: Synthetic
Genetic Insert: cTnT promoter (Cardiomyocyte specific)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pDONR221 P1-P5r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121696 Copy
Species: Synthetic
Genetic Insert: HA epitope tag (open)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2554; Vector Backbone:pDONR221 P5-P4; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121699 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121732 Copy
Species: Synthetic
Genetic Insert: mCherry (open)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121733 Copy
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