Species: Homo sapiens
Genetic Insert: Oculocerebrorenal syndrome of Lowe
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Bacterial Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22207 Copy
Species: Homo sapiens
Genetic Insert: MLH1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10200; Vector Backbone:pCEP9; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16458 Copy
Species: Mus musculus
Genetic Insert: EB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5443; Vector Backbone:tdTomato-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: We received the EB1 construct from Yuko Mimori-Kiyosue (RIKEN, Kobe, Japan) originally and replaced the GFP with tdTomato.
There is a 4x Gly linker between the EB1 and the tdTomato.
Proper citation: RRID:Addgene_50825 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB-MeCP2
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_110821 Copy
Species: Other
Genetic Insert: Domain 4 of Anthrolysin O
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2887; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111026 Copy
Species: Synthetic
Genetic Insert: dLight1.1
Vector Backbone Description: Backbone Size:5020; Vector Backbone:pAAV-CAG; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: Addgene NGS identified chicken beta-actin promoter, but not the CMV enhancer and chimeric intron often associated with CAG promoters. Please see the associated diagnostic digest for plasmid verification.
Proper citation: RRID:Addgene_111067 Copy
Species: Synthetic
Genetic Insert: dLight1.2
Vector Backbone Description: Backbone Size:4579; Vector Backbone:pAAV-hSyn; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111068 Copy
Species: Synthetic
Genetic Insert: dLight1.1
Vector Backbone Description: Backbone Size:3983; Vector Backbone:pEGFP_N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_111053 Copy
Species: Homo sapiens
Genetic Insert: human fission factor1
Vector Backbone Description: Backbone Marker:Covalys, NEB; Backbone Size:5325; Vector Backbone:pSEMS(26m); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: *Addgene QC found a V9E substitution in hFis1
Proper citation: RRID:Addgene_111136 Copy
Species: Homo sapiens
Genetic Insert: BAF47
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR223; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_111181 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pQCXIX; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111168 Copy
Species: Homo sapiens
Genetic Insert: hCAR
Vector Backbone Description: Backbone Size:4117; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111397 Copy
Species:
Genetic Insert: cyan pre-eGRASP(p30)
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111583 Copy
Species: Homo sapiens
Genetic Insert: ARHGAP29
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_104154 Copy
Species: Other
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_104105 Copy
Species: Synthetic
Genetic Insert: 24xMS2V6
Vector Backbone Description: Backbone Marker:Genescript; Vector Backbone:pUC57; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert generated by duplication of 12xMBSV6 as a BamHI/BglII fragment inserted at the BglII site
Proper citation: RRID:Addgene_104391 Copy
Species: Other
Genetic Insert: rpoA, B, C, and Z
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET; Vector Types:Bacterial Expression, Other, E. coli expression vector; Bacterial Resistance:Ampicillin
References:
Comments: pIA900 (rpoA, B, C, and Z) encodes a C-terminally His-tagged β′ which can be removed using TEV protease. Linker with TEV-His10 cloned between NheI and StuI sites of pIA787; NheI site is gone
Proper citation: RRID:Addgene_104401 Copy
Species: Homo sapiens
Genetic Insert: TDP-43
Vector Backbone Description: Vector Backbone:pJ4M; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_104480 Copy
Species: Other
Genetic Insert: RCaMP1h
Vector Backbone Description: Vector Backbone:pCIG2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_105013 Copy
Species: Other
Genetic Insert: G-CEPIA1er
Vector Backbone Description: Vector Backbone:pCIG2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_105012 Copy
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