Searching the RRID Resource Information Network
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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pcDNA3-mSmo-WT-V5 Resource Report Resource Website |
RRID:Addgene_126415 | m-Smoothened | Mus musculus | Ampicillin | PMID:26291458 | Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:22:25 | 0 | ||
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pCDNA3 mSmoothened NQ3 Resource Report Resource Website |
RRID:Addgene_126406 | m-Smoothened | Mus musculus | Ampicillin | PMID:26291458 | Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Smo glycosylation site NQ3 | 2022-04-22 03:22:24 | 0 | |
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pBS C-ret Resource Report Resource Website |
RRID:Addgene_119900 | ret | Mus musculus | Ampicillin | PMID:20064391 | This plasmid can be used to generate an in situ hybridization antisense probe for mouse C-ret. Linearize the plasmid with NotI and use T7 to transcribe the ~1kb probe. | Vector Backbone:pBS; Vector Types:Other, in situ hybridization probe; Bacterial Resistance:Ampicillin | 2022-04-22 03:20:26 | 0 | |
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mCh-KIF1A*-strep Resource Report Resource Website |
RRID:Addgene_120165 | kinesin family member 1A | Mus musculus | Kanamycin | PMID:31100061 | Backbone Marker:Clontech; Backbone Size:4727; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Truncated KIF1A: 1-405 aa | 2022-04-22 03:20:33 | 0 | |
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mCh-BICD2*-strep Resource Report Resource Website 1+ mentions |
RRID:Addgene_120168 | BICD cargo adaptor 2 | Mus musculus | Kanamycin | PMID:31100061 | Backbone Marker:Clontech; Backbone Size:4713; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Truncated BICD2: 15-595 aa | 2022-04-22 03:20:33 | 1 | |
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pLV-EF1a-V5-Tmem128 Resource Report Resource Website |
RRID:Addgene_120239 | Tmem128 | Mus musculus | Ampicillin | PMID: | Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2022-04-22 03:20:35 | 0 | ||
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jk96 Resource Report Resource Website |
RRID:Addgene_120177 | CAG:mCherry:P2A:HA_NLGN | Mus musculus | Ampicillin | PMID: | Vector Backbone:paavCAG-Jx; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:20:33 | 0 | ||
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pRK5 FLAG membrane-bound lipin-1 (TMD) Resource Report Resource Website |
RRID:Addgene_120277 | Lipin-1 | Mus musculus | Ampicillin | PMID:30718857 | Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:20:37 | 0 | ||
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pRK5 FLAG membrane-bound lipin-1 (delta200) Resource Report Resource Website |
RRID:Addgene_120278 | Lipin-1 | Mus musculus | Ampicillin | PMID:30718857 | Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:20:37 | 0 | ||
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PB-TAC-OKMS-Cas9 Resource Report Resource Website |
RRID:Addgene_120353 | OKMS cassette | Mus musculus | Ampicillin | PMID:30639212 | Please Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to depositor's provided sequence for this section of the plasmid. | Vector Backbone:PB-TAC-Cas9; Vector Types:Mammalian Expression, CRISPR, Other, piggyBac transposon; Bacterial Resistance:Ampicillin | Klf4 [10-483] | 2022-04-22 03:20:39 | 0 |
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PB-TAC-OK+9MS-Cas9 Resource Report Resource Website |
RRID:Addgene_120354 | OK+9MS cassette | Mus musculus | Ampicillin | PMID:30639212 | Vector Backbone:PB-TAC-Cas9; Vector Types:Mammalian Expression, CRISPR, Other, piggyBac transposon; Bacterial Resistance:Ampicillin | Klf4 [1-483] | 2022-04-22 03:20:39 | 0 | |
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qi5 Resource Report Resource Website |
RRID:Addgene_24501 | Gq alpha | Mus musculus | Ampicillin and Tetracycline | PMID:8387644 | This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gi alpha residues (EYNLV to DCGLF). This construct allows many Gi-coupled receptors to stimulate phospholipase C (PLC). This is the most popular chimera, perhaps because it is easier to talk about coupling to Gi-coupled receptors with "qi5" rather than "qo5" or "qz5." Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. | Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | Gq alpha with the C-terminal amino acids changed from Gq alpha to Gi alpha residues (EYNLV to DCGLF) | 2022-09-09 01:02:49 | 0 |
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qo5 Resource Report Resource Website |
RRID:Addgene_24500 | Gq alpha | Mus musculus | Ampicillin and Tetracycline | PMID:8387644 | This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues (EYNLV to GCGLY). Works the same as qi5 but (for unknown reasons) has a slightly lower basal PLC activity. This can increase the signal-to-noise ratio. Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. | Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | Gq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues (EYNLV to GCGLY) | 2022-09-09 01:02:49 | 0 |
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pcDNA-SUN2 mApple Resource Report Resource Website |
RRID:Addgene_187679 | SUN2 | Mus musculus | Ampicillin | PMID: | Backbone Marker:Invitrogen; Vector Backbone:pcDNA6.2-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | WT | 2022-09-09 01:02:38 | 0 | |
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pPBbsr2-Gnaq Resource Report Resource Website |
RRID:Addgene_170298 | A recombinant mouse Gnaq | Mus musculus | Ampicillin | PMID:34035084 | Vector Backbone:pPB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Asilent mutation was introduced into the sgRNA-targeted region | 2022-09-09 01:02:10 | 0 | |
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qs5 Resource Report Resource Website |
RRID:Addgene_24498 | Gq alpha | Mus musculus | Ampicillin and Tetracycline | PMID:8863834 | This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gs alpha residues (EYNLV to QYELL). This construct allows some Gs-coupled receptors to stimulate phospholipase C. Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. | Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | Replacement of the five carboxyl-terminal amino acids of Gq alpha (EYNLV) with those of Gs alpha(QYELL) | 2022-09-09 01:02:49 | 0 |
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pMCh1807_pBS_cdc42E7-boxB Resource Report Resource Website |
RRID:Addgene_118612 | cdc42E7 3'UTR | Mus musculus | Ampicillin | PMID:30590745 | Please note: Addgene NGS unable to fully resolve the poly-A tail length. Please refer to the depositor's sequence for this section of the plasmid. | Backbone Size:3311; Vector Backbone:pBS; Vector Types:Other, in vitro transcription; Bacterial Resistance:Ampicillin | 2022-09-21 01:00:45 | 0 | |
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Adeno FT Resource Report Resource Website |
RRID:Addgene_101824 | U6_sgRNA(Fgfr3)_U6_sgRNA(Tacc3)_CBh_FLAG-Cas9 | Mus musculus | Ampicillin | PMID:28695888 | Backbone Marker:Gene Transfer Vector Core University of Iowa; Backbone Size:5679; Vector Backbone:pacAd5; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin | 2022-09-21 01:00:11 | 0 | ||
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mApple-proα1(I) Resource Report Resource Website |
RRID:Addgene_119844 | Type I procollagen α1 chain | Mus musculus | Ampicillin | PMID:30287488 | These mouse type I procollagen plasmids were designed for immortalized and primary murine osteoblasts and fibroblasts. Their expression in other cells might cause significant disruption of assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in osteoblasts and fibroblasts a fraction of transfected chains, particularly proα2, might be trafficked/degraded as monomers instead of being integrated into procollagen heterotrimers. In our experience, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12 h after transfection. Fluorescent molecules should be incorporated into fibers even when the transfected chains contain the N-propeptide cleavage site, because the cleavage is highly conformation sensitive and always incomplete. We recommend following these guidelines when using the plasmids 1) Only mouse cells that have high expression of endogenous type I procollagen are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid excessive accumulation of aggregates and cell malfunction caused by increased procollagen synthesis. 5) Lack of extracellular fluorescent fibers 12-24 h after transfection indicates improper procollagen synthesis/trafficking or cellular malfunction. 6) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari (shakib.omari@nih.gov), Sergey Leikin (leikins@mail.nih.gov). | Backbone Size:5300; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Col1a1 exons 2-3 replaced by fluorescent tag | 2022-09-21 01:00:48 | 0 |
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pAAV-U6-Diras2_1-hSyn::mCherry.3xFLAG-WPRE Resource Report Resource Website |
RRID:Addgene_120393 | Diras2 shRNA | Mus musculus | Ampicillin | PMID:30623719 | The original backbone was published in Candemir et al. Eur Neuropsychopharmacol. 2016 (PMID: 26861996) | Backbone Size:4000; Vector Backbone:pAAV; Vector Types:AAV, RNAi; Bacterial Resistance:Ampicillin | 2022-09-21 01:00:49 | 0 |
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