Searching the RRID Resource Information Network
Species: Arabidopsis thaliana
Genetic Insert: SOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal
Vector Backbone Description: Vector Backbone:pRI 201-AN; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: The Eevee Linker present in this plasmid was invented by Prof. Michiyuki Matsuda at Kyoto University. The Eevee Linker has been patented by Kyoto University. The plasmid backbone used to produce the SOMA sensor was a kind gift of Michiyuki Matsuda and Kazuhiro Aoki. Please note there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied Genbank sequence. The authors have noted that the discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_118939 Copy
Species: Arabidopsis thaliana
Genetic Insert: pVND7:GR-LhG4:tVND7:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_116010 Copy
Species: Arabidopsis thaliana
Genetic Insert: pWOX4:GR-LhG4:tWOX4:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_116012 Copy
Species: Arabidopsis thaliana
Genetic Insert: pPXY
Vector Backbone Description: Vector Backbone:A000; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_116017 Copy
Species: Arabidopsis thaliana
Genetic Insert: pNST3
Vector Backbone Description: Vector Backbone:A000; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_116018 Copy
Species: Arabidopsis thaliana
Genetic Insert: pOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_116006 Copy
Species: Arabidopsis thaliana
Genetic Insert: pSMXL5:GR-LhG4:tSMXL5:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_116007 Copy
Species: Arabidopsis thaliana
Genetic Insert: pNST3:GR-LhG4:tNST3:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_116009 Copy
Species: Arabidopsis thaliana
Genetic Insert: pAHP6:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_115998 Copy
Species: Arabidopsis thaliana
Genetic Insert: Auxin Signaling Receptor AFB2
Vector Backbone Description: Vector Backbone:pGP8GF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_188426 Copy
Species: Arabidopsis thaliana
Genetic Insert: Auxin Signaling Receptor TIR1
Vector Backbone Description: Vector Backbone:pGP8GF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_188427 Copy
Species: Arabidopsis thaliana
Genetic Insert: sgRNA cassette under U6 promoter
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
References:
Comments: This vector was designed and constructed by Dr. Tomasz Bieluszewski.
Detailed protocol for CRISPR/Cas9-based mutagenesis using this system in:
Bieluszewski T, Szymanska-Lejman M, Dziegielewski W, Zhu L, Ziolkowski PA. Efficient Generation of CRISPR/Cas9-Based Mutants Supported by Fluorescent Seed Selection in Different Arabidopsis Accessions, in: Plant Gametogenesis: Methods and Protocols (ed. Christophe Lambing), Methods in Molecular Biology, vol. 2484.
https://doi.org/10.1007/978-1-0716-2253-7_13
https://pubmed.ncbi.nlm.nih.gov/35461452/
Proper citation: RRID:Addgene_173157 Copy
Species: Arabidopsis thaliana
Genetic Insert: Arabidopsis thaliana Atm3
Vector Backbone Description: Backbone Size:5340; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_172321 Copy
Species: Arabidopsis thaliana
Genetic Insert: 5X Myc-Spacers-Auxin Inducible Degron for N-terminal attachment
Vector Backbone Description: Backbone Size:2710; Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Submitted the 5X Myc-Spacers+Degron sequence (see below) to Genscript for GenePlus Economy synthesis and cloning into the EcoRV site of pUC57.
Proper citation: RRID:Addgene_110913 Copy
Species: Arabidopsis thaliana
Genetic Insert: CRY2(neutral2-6)-mCherry
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_104181 Copy
Species: Arabidopsis thaliana
Genetic Insert: CRY2low-mCherry
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_104065 Copy
Species: Arabidopsis thaliana
Genetic Insert: sgRNA-transRNA transcription by U6 promoter
Vector Backbone Description: Backbone Marker:Lampropoulos et al., 2013; Vector Backbone:pE000; Vector Types:Bacterial Expression, Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_104441 Copy
Species: Arabidopsis thaliana
Genetic Insert: sgRNA-transRNA transcription by U6 promoter
Vector Backbone Description: Backbone Marker:Lampropoulos et al., 2013; Vector Backbone:pD000; Vector Types:Bacterial Expression, Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_104440 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cry2-mCherry-PKA W196R/F327A
Vector Backbone Description: Backbone Marker:Chandra Tucker; Backbone Size:6219; Vector Backbone:pmCherryCry2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_104547 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cry2-mCherry-PKA W196R/Y204A
Vector Backbone Description: Backbone Marker:Chandra Tucker; Backbone Size:6219; Vector Backbone:pmCherryCry2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_104546 Copy
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