Plasmids   Select Another Resource Report Type

http://www.addgene.org/126415

Species: Mus musculus
Genetic Insert: m-Smoothened
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_126415 Copy   

•   Source: Addgene

http://www.addgene.org/126406

Species: Mus musculus
Genetic Insert: m-Smoothened
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_126406 Copy   

•   Source: Addgene

http://www.addgene.org/119900

Species: Mus musculus
Genetic Insert: ret
Vector Backbone Description: Vector Backbone:pBS; Vector Types:Other, in situ hybridization probe; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid can be used to generate an in situ hybridization antisense probe for mouse C-ret. Linearize the plasmid with NotI and use T7 to transcribe the ~1kb probe.

Proper citation: RRID:Addgene_119900 Copy   

•   Source: Addgene

http://www.addgene.org/120165

Species: Mus musculus
Genetic Insert: kinesin family member 1A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4727; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_120165 Copy   

•   Source: Addgene

http://www.addgene.org/120168

Species: Mus musculus
Genetic Insert: BICD cargo adaptor 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4713; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_120168 Copy   

•   Source: Addgene

http://www.addgene.org/120239

Species: Mus musculus
Genetic Insert: Tmem128
Vector Backbone Description: Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_120239 Copy   

•   Source: Addgene

http://www.addgene.org/120177

Species: Mus musculus
Genetic Insert: CAG:mCherry:P2A:HA_NLGN
Vector Backbone Description: Vector Backbone:paavCAG-Jx; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_120177 Copy   

•   Source: Addgene

http://www.addgene.org/120277

Species: Mus musculus
Genetic Insert: Lipin-1
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_120277 Copy   

•   Source: Addgene

http://www.addgene.org/120278

Species: Mus musculus
Genetic Insert: Lipin-1
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_120278 Copy   

•   Source: Addgene

http://www.addgene.org/120353

Species: Mus musculus
Genetic Insert: OKMS cassette
Vector Backbone Description: Vector Backbone:PB-TAC-Cas9; Vector Types:Mammalian Expression, CRISPR, Other, piggyBac transposon; Bacterial Resistance:Ampicillin
References:
Comments: Please Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to depositor's provided sequence for this section of the plasmid.

Proper citation: RRID:Addgene_120353 Copy   

•   Source: Addgene

http://www.addgene.org/120354

Species: Mus musculus
Genetic Insert: OK+9MS cassette
Vector Backbone Description: Vector Backbone:PB-TAC-Cas9; Vector Types:Mammalian Expression, CRISPR, Other, piggyBac transposon; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_120354 Copy   

•   Source: Addgene

http://www.addgene.org/24501

Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gi alpha residues (EYNLV to DCGLF). This construct allows many Gi-coupled receptors to stimulate phospholipase C (PLC). This is the most popular chimera, perhaps because it is easier to talk about coupling to Gi-coupled receptors with "qi5" rather than "qo5" or "qz5." Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual.

Proper citation: RRID:Addgene_24501 Copy   

•   Source: Addgene

http://www.addgene.org/24500

Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues (EYNLV to GCGLY). Works the same as qi5 but (for unknown reasons) has a slightly lower basal PLC activity. This can increase the signal-to-noise ratio. Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual.

Proper citation: RRID:Addgene_24500 Copy   

•   Source: Addgene

http://www.addgene.org/187679

Species: Mus musculus
Genetic Insert: SUN2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA6.2-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_187679 Copy   

•   Source: Addgene

http://www.addgene.org/170298

Species: Mus musculus
Genetic Insert: A recombinant mouse Gnaq
Vector Backbone Description: Vector Backbone:pPB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_170298 Copy   

•   Source: Addgene

http://www.addgene.org/24498

Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gs alpha residues (EYNLV to QYELL). This construct allows some Gs-coupled receptors to stimulate phospholipase C. Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual.

Proper citation: RRID:Addgene_24498 Copy   

•   Source: Addgene

http://www.addgene.org/118612

Species: Mus musculus
Genetic Insert: cdc42E7 3'UTR
Vector Backbone Description: Backbone Size:3311; Vector Backbone:pBS; Vector Types:Other, in vitro transcription; Bacterial Resistance:Ampicillin
References:
Comments: Please note: Addgene NGS unable to fully resolve the poly-A tail length. Please refer to the depositor's sequence for this section of the plasmid.

Proper citation: RRID:Addgene_118612 Copy   

•   Source: Addgene

http://www.addgene.org/101824

Species: Mus musculus
Genetic Insert: U6_sgRNA(Fgfr3)_U6_sgRNA(Tacc3)_CBh_FLAG-Cas9
Vector Backbone Description: Backbone Marker:Gene Transfer Vector Core University of Iowa; Backbone Size:5679; Vector Backbone:pacAd5; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_101824 Copy   

•   Source: Addgene

http://www.addgene.org/119844

Species: Mus musculus
Genetic Insert: Type I procollagen α1 chain
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: These mouse type I procollagen plasmids were designed for immortalized and primary murine osteoblasts and fibroblasts. Their expression in other cells might cause significant disruption of assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in osteoblasts and fibroblasts a fraction of transfected chains, particularly proα2, might be trafficked/degraded as monomers instead of being integrated into procollagen heterotrimers. In our experience, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12 h after transfection. Fluorescent molecules should be incorporated into fibers even when the transfected chains contain the N-propeptide cleavage site, because the cleavage is highly conformation sensitive and always incomplete. We recommend following these guidelines when using the plasmids 1) Only mouse cells that have high expression of endogenous type I procollagen are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid excessive accumulation of aggregates and cell malfunction caused by increased procollagen synthesis. 5) Lack of extracellular fluorescent fibers 12-24 h after transfection indicates improper procollagen synthesis/trafficking or cellular malfunction. 6) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari (shakib.omari@nih.gov), Sergey Leikin (leikins@mail.nih.gov).

Proper citation: RRID:Addgene_119844 Copy   

•   Source: Addgene

http://www.addgene.org/120393

Species: Mus musculus
Genetic Insert: Diras2 shRNA
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pAAV; Vector Types:AAV, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: The original backbone was published in Candemir et al. Eur Neuropsychopharmacol. 2016 (PMID: 26861996)

Proper citation: RRID:Addgene_120393 Copy   

•   Source: Addgene