Species: Mus musculus
Genetic Insert: Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1/V5-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_122681 Copy
Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations A74V, M82I and H202Y when compared to GenBank reference NP_036035.3. M82I and H202Y are possible polymorphisms as they were also present in the wild-type TLR2 sequence from the depositing laboratory (Addgene Plasmid 12291: pCMV-M2-TLR2). It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12290 Copy
Species: Mus musculus
Genetic Insert: m(KS_MAT)_mouseL_ACP
Vector Backbone Description: Backbone Marker:Novagen/Merck Millipore; Backbone Size:5340; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_122850 Copy
Species: Mus musculus
Genetic Insert: mouse FASN/ mouse fatty acid synthase
Vector Backbone Description: Backbone Marker:Novagen/Merck Millipore; Backbone Size:5340; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_122847 Copy
Species: Mus musculus
Genetic Insert: m(KS_MAT)
Vector Backbone Description: Backbone Marker:Novagen/Merck Millipore; Backbone Size:5340; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_122849 Copy
Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations M82I and H202Y when compared to GenBank reference NP_036035.3. It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12291 Copy
Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations M82I and H202Y when compared to GenBank reference NP_036035.3. M82I and H202Y are possible polymorphisms as they were also present in the wild-type TLR2 sequence from the depositing laboratory (Addgene Plasmid 12291: pCMV-M2-TLR2). It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12293 Copy
Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations M82I and H202Y when compared to GenBank reference NP_036035.3. M82I and H202Y are possible polymorphisms as they were also present in the wild-type TLR2 sequence from the depositing laboratory (Addgene Plasmid 12291: pCMV-M2-TLR2). It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12292 Copy
Species: Mus musculus
Genetic Insert: PACT
Vector Backbone Description: Vector Backbone:pSV40_HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_120537 Copy
Species: Mus musculus
Genetic Insert: TARBP2
Vector Backbone Description: Vector Backbone:pSV40_HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_120539 Copy
Species: Mus musculus
Genetic Insert: Mos
Vector Backbone Description: Vector Backbone:pcDNA3.1(-); Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments: A>G mutation in Mos mRNA does not affect plasmid function.
Proper citation: RRID:Addgene_120525 Copy
Species: Mus musculus
Genetic Insert: Mos
Vector Backbone Description: Vector Backbone:pJET1.2; Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_120529 Copy
Species: Mus musculus
Genetic Insert: Dicer
Vector Backbone Description: Vector Backbone:pEF1alpha.Blast; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_120541 Copy
Species: Mus musculus
Genetic Insert: ptch1
Vector Backbone Description: Vector Backbone:pVLAD6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_120886 Copy
Species: Mus musculus
Genetic Insert: Ptch1
Vector Backbone Description: Vector Backbone:pcDNA-h; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_120904 Copy
Species: Mus musculus
Genetic Insert: Ptch1
Vector Backbone Description: Vector Backbone:pcDNA-h; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_120905 Copy
Species: Mus musculus
Genetic Insert: ELK1
Vector Backbone Description: Vector Backbone:pcDNA3.1 HisC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: see attached PDF of sequence
Proper citation: RRID:Addgene_121095 Copy
Species: Mus musculus
Genetic Insert: ELK1
Vector Backbone Description: Vector Backbone:pcDNA3.1 HisC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: see atctehd PDF of sequence
Proper citation: RRID:Addgene_121096 Copy
Species: Mus musculus
Genetic Insert: SrtA-PDGFR
Vector Backbone Description: Backbone Size:6200; Vector Backbone:pMP71; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121168 Copy
Species: Mus musculus
Genetic Insert: Fndc3b
Vector Backbone Description: Vector Backbone:pcDNA3.1(+) Laccase2 MCS Exon; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_126444 Copy
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