Searching the RRID Resource Information Network
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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W999F Resource Report Resource Website |
RRID:Addgene_52712 | W999F strain | None | PMID:22605776 | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:44 | 0 | |||
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delta-ZYA Resource Report Resource Website |
RRID:Addgene_52695 | ΔZYA strain | None | PMID:22605776 | MG1655 was transformed with pKD46 (carrying phage lambda Red recombinase) and a PCR fragment encoding a kanamycin resistance gene with flanking nucleotide sequences homologous to those flanking the lac operon. Upon recombination and selection for resistant strains, the kanamycin-resistant gene was eliminated following transformation with pCP20 (encoding the FLP recombinase), which is subsequently cured by growth at 30°C. The deletion in our ΔZYA strain started 20bp upstream of lacI and ran 40bp downstream of lacA. | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:43 | 0 | ||
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OpLac1-delta-6 Resource Report Resource Website |
RRID:Addgene_52698 | OpLac1-Δ6 strain | None | PMID:22605776 | Oplac1Δ6 were erroneously synthesized missing the first 6 nucleotides of Oplac1. These deletions correspond to the first 2 N-terminal amino acid residues (Methionine and Threonine), and instead begin at the Methionine at position 3. | Vector Backbone:na; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:43 | 0 | ||
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HL3721 Resource Report Resource Website |
RRID:Addgene_52950 | none | Other | None | PMID:23878244 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-05 07:56:46 | 0 | ||
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Top10∆serB Resource Report Resource Website |
RRID:Addgene_34928 | None | PMID:21868676 | To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which catalyzes the last step in serine biosynthesis, was deleted from Escherichia coli strain Top10. Markerless gene deletions were carried out using a λ-red and FLP recombinase-based gene knockout strategy. | Vector Backbone:None; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:50:38 | 0 | |||
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HL 716 Resource Report Resource Website |
RRID:Addgene_60368 | See comments | None | PMID:21189298 | MG1655 + lacIq integrated at intS | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-05 07:59:29 | 0 | ||
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HL 770 Resource Report Resource Website |
RRID:Addgene_60369 | See comments | None | PMID:21189298 | MG1655 + lacIq intergrated at intS + Δ hfq | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-05 07:59:29 | 0 | ||
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RF2 Resource Report Resource Website |
RRID:Addgene_62070 | none | None | PMID:26577727 | Genotype = thrC Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Thr Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:39 | 0 | ||
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RF12 Resource Report Resource Website |
RRID:Addgene_62077 | none | None | PMID:26577727 | Genotype = trpA trpB Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Trp## ##RF12 strain requires Trp for growth in M63 minimal medium. For effective Trp labeling of a recombinant protein, the presence of 0.4-1 mM Tyr in growth medium to repress the tyrB gene is recommended. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:39 | 0 | ||
|
RF5 Resource Report Resource Website |
RRID:Addgene_62073 | none | None | PMID:26577727 | Genotype = aspC tyrB hisG Precursor strain = RF4 modified from the parent Escherichia coli BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Asp++++, Tyr, (Phe), His ++++RF4, RF5, and RF13 strains have knockouts in both aspC and tyrB genes and therefore require the presence of L-Asp (but not L-Glu) for growth in M63 minimal medium. These strains can be used for selective labeling of selected aromatic amino acid(s) such as Tyr (RF4, RF5, RF13) and/or Trp (RF13). We have NOT tested if these strains could also be used for selective Asp labeling studies (under investigation). Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:39 | 0 | ||
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HL 1188 Resource Report Resource Website |
RRID:Addgene_60373 | See comments | None | PMID:21189298 | MG1655 + lacIq intergrated at intS + Δ hfq + Δ dsrA | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-14 01:07:34 | 0 | ||
|
RF10 Resource Report Resource Website |
RRID:Addgene_62076 | none | None | PMID:26577727 | Genotype = lysA Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Lys Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:39 | 0 | ||
|
ML14 Resource Report Resource Website |
RRID:Addgene_61911 | none | None | PMID:21925267 | Genotype = tyrA Precursor strain = C43(DE3) modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Tyr) Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:38 | 0 | ||
|
ML17 Resource Report Resource Website |
RRID:Addgene_61912 | none | None | PMID:21925267 | Genotype = glnA Precursor strain = C43(DE3) modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = Gln Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:38 | 0 | ||
|
ML36 Resource Report Resource Website |
RRID:Addgene_61919 | none | None | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG metA Precursor strain = ML23 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Met *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:38 | 0 | ||
|
ML24 Resource Report Resource Website |
RRID:Addgene_61915 | none | None | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG asnA Precursor strain = ML23 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:38 | 0 | ||
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ML25 Resource Report Resource Website |
RRID:Addgene_61916 | none | None | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG asnA asnB Precursor strain = ML24 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Asn *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:38 | 0 | ||
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ML42 Resource Report Resource Website |
RRID:Addgene_61921 | none | None | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG argH metA lysA thrC asnB Precursor strain = ML41 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg, Met, Lys, Thr *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:38 | 0 | ||
|
RF11 Resource Report Resource Website |
RRID:Addgene_61961 | none | None | PMID:26577727 | Genotype = metA Precursor strain = C43(DE3) Selective amino acid labeling (and/or requirement) = Met Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:38 | 0 | ||
|
HL 2028 Resource Report Resource Website |
RRID:Addgene_61163 | See comments | None | PMID:22833608 | HL1745 + PLlacO-1::T710::yfp intergrated at galK+ ∆lacI | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-14 01:07:36 | 0 |
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