Species: Synthetic
Genetic Insert: VanRAM-mRFP
Vector Backbone Description: Vector Backbone:pIVR5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167510 Copy
Species: Synthetic
Genetic Insert: CinRAM-mKelly2
Vector Backbone Description: Vector Backbone:pFCiK5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167514 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:ATCC 10326362; Backbone Size:4608; Vector Backbone:pENTR1A-Gent; Vector Types:Other, Entry vector; Bacterial Resistance:Gentamicin
References:
Comments: shRNA expression; miR30-based topology; TRE Pmin promoter; Entry vector backbone; +ccdB in parent; Multiple unique sites d/s of TRE
Proper citation: RRID:Addgene_25748 Copy
Species: Synthetic
Genetic Insert: LuxR-tdKatushka2
Vector Backbone Description: Vector Backbone:pFLxTd5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167519 Copy
Species: Synthetic
Genetic Insert: LuxR-Violacein
Vector Backbone Description: Vector Backbone:pBLxVi5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167516 Copy
Species: Other
Genetic Insert: Q22YU3 C-terminal truncation (Delta-C3)
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_170316 Copy
Species: Synthetic
Genetic Insert: J306 scRNA
Vector Backbone Description: Backbone Marker:Jesse Zalatan; Backbone Size:6261; Vector Backbone:pPPC020.306; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
References:
Comments: Alternative names: pCK134, pBBR1-GmR_J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
Proper citation: RRID:Addgene_171149 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: Gm cassette (BssHII+SphI)
Vector Backbone Description: Vector Backbone:pAM1303; Vector Types:Other, Cyanobacteria cloning vector; Bacterial Resistance:Gentamicin
References:
Comments: NS1 vector
Gm resistant version of pAM1303.
Both pAM1303 and pAM3511 were digested with BssHII and SphI, and then mix-ligated. The accC1gene (Gm) replaces the aadA gene (SpSm) in the Ω cassette. The upstream T4 terminator was lost due to SphI digestion.
Proper citation: RRID:Addgene_40252 Copy
Species: Arabidopsis thaliana
Genetic Insert: ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Other, Gateway Donor vector / entry clone; Bacterial Resistance:Gentamicin
References:
Comments: For use with Three-way Multi-site Gateway Cloning (Invitrogen).
Proper citation: RRID:Addgene_170723 Copy
Species: Other
Genetic Insert: Rhamnogalacturonan lyase B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Other, Gateway Donor vector / entry clone; Bacterial Resistance:Gentamicin
References:
Comments: For use with Three-way Multi-site Gateway Cloning (Invitrogen).
Proper citation: RRID:Addgene_170721 Copy
Species: Other
Genetic Insert: Pectin lyase A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Other, Gateway Donor vector / entry clone; Bacterial Resistance:Gentamicin
References:
Comments: For use with Three-way Multi-site Gateway Cloning (Invitrogen).
Proper citation: RRID:Addgene_170722 Copy
Species:
Genetic Insert: Tobacco rattle virus RNA1
Vector Backbone Description: Vector Backbone:pLX-Z4; Vector Types:Plant Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_180515 Copy
Species: Synthetic
Genetic Insert: non-targeting shRNA
Vector Backbone Description: Backbone Marker:ATCC 10326362 ; Backbone Size:4232; Vector Backbone:pEN_TmiRc3 (pENTR1A-Gent); Vector Types:Other, Cloning; Bacterial Resistance:Gentamicin
References:
Comments: For use with pSLIK lentiviral system. Original publication:
A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, Rebres R, Roach T, Seaman W, Simon MI, Fraser ID. Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64. 10.1073/pnas.0606179103
Proper citation: RRID:Addgene_81067 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Beckwith Lab; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_65098 Copy
Species: Other
Genetic Insert: ugi
Vector Backbone Description: Backbone Size:6041; Vector Backbone:pPSV39-CV; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_186556 Copy
Species: Other
Genetic Insert: dddAI
Vector Backbone Description: Backbone Size:6041; Vector Backbone:pPSV39-CV; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_186558 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: GenBank Accession # DQ296496
Compared to the Genbank reference sequence DQ296496, Addgene's sequencing results found T920I, D999E and F1000L mutations in lacZ. The effect of these differences is unknown. Note that the amino acid numbering is relative to the lacZ encoded by this plasmid.
Proper citation: RRID:Addgene_65028 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18R6K-mini-Tn7T; Vector Types:; Bacterial Resistance:Gentamicin
References:
Comments: GenBank Accession # AY599228
Proper citation: RRID:Addgene_65022 Copy
Species: Other
Genetic Insert: E2-Crimson
Vector Backbone Description: Vector Backbone:pUCP30T; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: There is an ER retention signal at the C-terminus of the E2-Crimson ORF. This sequence is based on the template used to generate this vector. It is not predicted to impact expression or function in bacteria.
Proper citation: RRID:Addgene_78478 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: See J. Microbio. Methods 2015 118:75-79 for details
The authors used this vector along with a Tn7 transposase encoding plasmid, pTNS1.
Proper citation: RRID:Addgene_70115 Copy
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