Plasmids   Select Another Resource Report Type

http://www.addgene.org/91570

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:4559; Vector Backbone:pSAM_Ec; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Mariner himar1C9 transposase driven by lac promoter on an R6K gamma suicide plasmid. The transposon allows identification of fitness factors by Illumina sequencing. This transposon will randomly insert a kanamycin resistance cassette into genomic TA sites. Chloramphenicol resistance is contained on the plasmid backbone. For further background, see: Goodman, Andrew L., Meng Wu, and Jeffrey I. Gordon. “Identifying Microbial Fitness Determinants by Insertion Sequencing (INSeq) Using Genome-Wide Transposon Mutant Libraries.” Nature Protocols 6.12 (2011): 1969–1980. PMC. Web. 11 Apr. 2017. Wiles, Travis J. et al. “Combining Quantitative Genetic Footprinting and Trait Enrichment Analysis to Identify Fitness Determinants of a Bacterial Pathogen.” Ed. Danielle A. Garsin. PLoS Genetics 9.8 (2013): e1003716. PMC. Web. 11 Apr. 2017.

Proper citation: RRID:Addgene_91570 Copy   

•   Source: Addgene

http://www.addgene.org/110080

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMEXC3H; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_110080 Copy   

•   Source: Addgene

http://www.addgene.org/110074

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMINTNH3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_110074 Copy   

•   Source: Addgene

http://www.addgene.org/49399

Species: Mus musculus
Genetic Insert: Olig2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pLP-IRES2-EGFP; Vector Types:Mammalian Expression, Cre/Lox, Other, acceptor vector; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: mOlig2 was transferred from the donor vector (pDNR-mOlig2, Addgene plasmid #49397) to the acceptor vector using Cre-mediated recombination at the LoxP sites.

Proper citation: RRID:Addgene_49399 Copy   

•   Source: Addgene

http://www.addgene.org/49391

Species: Homo sapiens
Genetic Insert: Sox2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pLP-IRES2-EGFP; Vector Types:Mammalian Expression, Cre/Lox, Other, acceptor vector; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: hSox2 was transferred from the donor vector (pDNR-hSox2, Addgene plasmid #49389) to the acceptor vector using Cre-mediated recombination at the LoxP sites.

Proper citation: RRID:Addgene_49391 Copy   

•   Source: Addgene

http://www.addgene.org/61655

Species: Other
Genetic Insert: enhanced yellow fluorescent protein
Vector Backbone Description: Backbone Marker:Cambia, Canberra, Australia; Backbone Size:6000; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_61655 Copy   

•   Source: Addgene

http://www.addgene.org/61705

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:11775; Vector Backbone:pEarleyGate-202; Vector Types:Plant Expression, Other, BiFC assay; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_61705 Copy   

•   Source: Addgene

http://www.addgene.org/122574

Species: Synthetic
Genetic Insert: catP/sfGFP
Vector Backbone Description: Backbone Size:4200; Vector Backbone:pGT-Ah7; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: See supplemental tables of the referenced paper for more information on each plasmid.

Proper citation: RRID:Addgene_122574 Copy   

•   Source: Addgene

http://www.addgene.org/110081

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMEXC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_110081 Copy   

•   Source: Addgene

http://www.addgene.org/126054

Species: Other
Genetic Insert: chloroplastic FNR transit peptide
Vector Backbone Description: Vector Backbone:pAGM4723; Vector Types:Plant Expression, Other, Binary; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Please incubate bacteria at 30 degrees Celsius after transformations to increase efficiency. Further growth after transformants are obtained should be performed at 37 degrees Celsius.

Proper citation: RRID:Addgene_126054 Copy   

•   Source: Addgene

http://www.addgene.org/126055

Species: Other
Genetic Insert: chloroplastic FNR transit peptide
Vector Backbone Description: Vector Backbone:pAGM4723; Vector Types:Plant Expression, Other, Binary; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Please incubate bacteria at 30 degrees Celsius after transformations to increase efficiency. Further growth after transformants are obtained should be performed at 37 degrees Celsius.

Proper citation: RRID:Addgene_126055 Copy   

•   Source: Addgene

http://www.addgene.org/18671

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7040; Vector Backbone:pEGFPC4; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: pEGFPC4 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal EGFP tag.

Proper citation: RRID:Addgene_18671 Copy   

•   Source: Addgene

http://www.addgene.org/24510

Species: Aequorea victoria (Jellyfish)
Genetic Insert: Yn, Yc, ECFP, EYFP
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:7434; Vector Backbone:pVitro2; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Note: This plasmid has been partially sequenced. Due to the presence of repeated features in the plasmid that prohibit thorough sequencing, the provided sequence should be considered theoretical and could contain errors. Please refer to Lu, Jian-Ping, Beatty, Laura, and Pinthus, Jehonathan . Dual expression recombinase based (DERB) single vector system for high throughput screening and verification of protein interactions in living cells. Available from Nature Precedings (2008) http://precedings.nature.com/documents/1550/version/2

Proper citation: RRID:Addgene_24510 Copy   

•   Source: Addgene

http://www.addgene.org/89487

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMDC43; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: To enhance folding of the fusion proteins a flexible linker was introduced between the coding sequence of either fragment of YFP and the attR recombination site. For more information see: http://www.ibmcp.upv.es/FerrandoLabVectors/

Proper citation: RRID:Addgene_89487 Copy   

•   Source: Addgene

http://www.addgene.org/199083

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: For standard propagation, supplement LB growth medium with 2% glucose (w/v) to repress sfYFP dropout expression, otherwise escape mutants may arise.

Proper citation: RRID:Addgene_199083 Copy   

•   Source: Addgene

http://www.addgene.org/197782

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:derived from pUC19; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.

Proper citation: RRID:Addgene_197782 Copy   

•   Source: Addgene

http://www.addgene.org/199721

Species: Synthetic
Genetic Insert: NPTII
Vector Backbone Description: Backbone Marker:Yiping Qi; Vector Backbone:pYPQ210; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: A 185T mutation was found in mCherry.

Proper citation: RRID:Addgene_199721 Copy   

•   Source: Addgene

http://www.addgene.org/128440

Species: Synthetic
Genetic Insert: C-terminal V5 tag
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_128440 Copy   

•   Source: Addgene

http://www.addgene.org/128439

Species: Synthetic
Genetic Insert: C-terminal HA tag
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_128439 Copy   

•   Source: Addgene

http://www.addgene.org/128461

Species: Synthetic
Genetic Insert: CAMKIIA promoter and C-terminal V5 tag
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_128461 Copy   

•   Source: Addgene