Species: Other
Genetic Insert: SV40-polyA
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: SV40-polyA terminator sequence.
Proper citation: RRID:Addgene_100678 Copy
Species:
Genetic Insert: Fyn
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_100741 Copy
Species: Homo sapiens
Genetic Insert: AP2B1
Vector Backbone Description: Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_100736 Copy
Species: Other
Genetic Insert: FbGH17Bcat
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:5369; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_100912 Copy
Species: Other
Genetic Insert: Kanamycin casette (in place of spoT gene) flanked by S. elongatus sequence
Vector Backbone Description: Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Other, Cloning vector; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_100913 Copy
Species: Homo sapiens
Genetic Insert: RERE homology arms
Vector Backbone Description: Backbone Marker:Eric Mendenhall & Richard M. Myers (Addgene plasmid # 63934); Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
References:
Comments: Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_101069 Copy
Species: Homo sapiens
Genetic Insert: EED
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: EED:APC049-D10:C27444. SGC or PDB link: http://www.thesgc.org/structures/5TTW/
Proper citation: RRID:Addgene_101254 Copy
Species: Mus musculus
Genetic Insert: alpha-Catenin
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: The TS module with mTFP1-F40-venusA206K was adapted from Vinculin TS (a kind gift from Martin Schwartz [Grashoff et al., 2010]). The TSmod was cloned in to pEGFP-N1 vector at Xho1 and Not1 restriction sites. For aE-Cat-TS, the mouse N-terminal (1_697 aa) of alpha-catenin was inserted before mTFP1 by in- fusion cloning (Takara Bioscience, Clontech) at the Xho1 restriction site
Proper citation: RRID:Addgene_101298 Copy
Species: Mus musculus
Genetic Insert: alpha-Catenin
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: The C-terminal fragment (698-906 aa) of alpha-catenin was cloned after VenusA206K at the Not1 restriction site
Proper citation: RRID:Addgene_101299 Copy
Species: Homo sapiens
Genetic Insert: EPHA3
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26094); Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: N terminal tag: MGSSHHHHHHSSGLVPRGS. SGC Clone Sample ID: EPHA3:SDC033-F08:C12121. SGC or PDB link: http://www.rcsb.org/pdb/explore/explore.do?structureId=2QO2
Proper citation: RRID:Addgene_101271 Copy
Species: Homo sapiens
Genetic Insert: RPUSD4
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: RPUSD4:JMC111-H03:C235563. SGC or PDB link: http://www.thesgc.org/structures/5UBA/
Proper citation: RRID:Addgene_101256 Copy
Species: Homo sapiens
Genetic Insert: BSX
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Other, Gateway shuttling vector; Bacterial Resistance:Kanamycin
References:
Comments: Harvard generated by gene synthesis
Proper citation: RRID:Addgene_101410 Copy
Species: Homo sapiens
Genetic Insert: HES3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Other, Gateway shuttling vector; Bacterial Resistance:Kanamycin
References:
Comments: Harvard generated by gene synthesis
Proper citation: RRID:Addgene_101411 Copy
Species: Homo sapiens
Genetic Insert: WHSC1
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: WHSC1:JMC062-B09:C224576. SGC or PDB links:
https://www.thesgc.org/structures/5VC8/
https://www.thesgc.org/structures/7LMT/
Proper citation: RRID:Addgene_101262 Copy
Species: Other
Genetic Insert: PP-BRD4
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: PF14_0724:MAC045-C12:C221552. SGC or PDB link: http://www.thesgc.org/structures/5VS7/
Proper citation: RRID:Addgene_101264 Copy
Species: Homo sapiens
Genetic Insert: CXXC5
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: CXXC5:JMC02B-D02:C226464. SGC or PDB link: http://www.thesgc.org/structures/5W9S/
Proper citation: RRID:Addgene_101266 Copy
Species: Homo sapiens
Genetic Insert: HES5
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Other, Gateway shuttling vector; Bacterial Resistance:Kanamycin
References:
Comments: Harvard generated by gene synthesis
Proper citation: RRID:Addgene_101409 Copy
Species: Homo sapiens
Genetic Insert: HOXD8
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Other, Gateway shuttling vector; Bacterial Resistance:Kanamycin
References:
Comments: Harvard generated by gene synthesis
Proper citation: RRID:Addgene_101402 Copy
Species: Homo sapiens
Genetic Insert: ZNF251
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Other, Gateway shuttling vector; Bacterial Resistance:Kanamycin
References:
Comments: Harvard generated by gene synthesis
Proper citation: RRID:Addgene_101451 Copy
Species: Homo sapiens
Genetic Insert: ZNF234
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Other, Gateway shuttling vector; Bacterial Resistance:Kanamycin
References:
Comments: Harvard generated by gene synthesis
Proper citation: RRID:Addgene_101611 Copy
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