Searching the RRID Resource Information Network
Species: Synthetic
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Fluorescent reporter - Red - ?ex (nm) = 559 ?em (nm) = 600 . doi: 10.1038/nmeth.2171
Proper citation: RRID:Addgene_100687 Copy
Species: Homo sapiens
Genetic Insert: Pim1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_100722 Copy
Species: Homo sapiens
Genetic Insert: MTMR1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: The MTMR1 construct is for structural determination and contains the PH-GRAM and PTPase domains (amino acids 95-607). PTPase activity was confirmed.
Proper citation: RRID:Addgene_100723 Copy
Species: Homo sapiens
Genetic Insert: PGKpA
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: mouse phosphoglucokinase polyA. Terminator.
Proper citation: RRID:Addgene_100683 Copy
Species: Synthetic
Genetic Insert: Insulator_FB
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: FII/BEAD-A (FB) element. (STEMCELLS2008;26:3257�3266 ). FII/BEAD-A (FB) contains the minimal enhancer-blocking components of the chicken b-globin 5'HS4 insulator and a homologous region from the human T-cell receptor BEAD-1 insulator
IMPORT NOTES: Special character found in field(Comments)
Proper citation: RRID:Addgene_100686 Copy
Species: Other
Genetic Insert: CMV promoter
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Strong constitutive promoter : Human�cytomegalovirus�(CMV) immediate early enhancer and�promoter
IMPORT NOTES: Special character found in field(Comments)
Proper citation: RRID:Addgene_100690 Copy
Species: Other
Genetic Insert: PuroR
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Puromycin resistance gene ecnoding a puromycin N-acetyl-transferase.
Proper citation: RRID:Addgene_100691 Copy
Species: Other
Genetic Insert: PuroR
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Puromycin resistance gene ecnoding a puromycin N-acetyl-transferase.
Proper citation: RRID:Addgene_100692 Copy
Species: Synthetic
Genetic Insert: DmrC
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DmrC is a chemically inducible dimerization domain.It dimerizes with DmrA in presence of heterodimerizer. iDimerizeTM Inducible Heterodimer System - Clontech
Proper citation: RRID:Addgene_100693 Copy
Species: Synthetic
Genetic Insert: Linker1
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Flexible peptide linker: SGLRSGSGGGSASGGSG . PROTEIN SCIENCE 2013 VOL 22:153�167
IMPORT NOTES: Special character found in field(Comments)
Proper citation: RRID:Addgene_100699 Copy
Species: Homo sapiens
Genetic Insert: Kozak_ATG_Palm
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: N-terminal GAP43-palmitoylation sequence. Petide tag for targeting the protein to the plasma membrane
Proper citation: RRID:Addgene_100694 Copy
Species: Synthetic
Genetic Insert: Linker3
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Flexible peptide linker: GGGSGGGGSAS
Proper citation: RRID:Addgene_100695 Copy
Species: Other
Genetic Insert: P2A
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: p2A-Porcine teschovirus-1: self-cleaving� 2A peptide. Nat.Biotechnol.2004. 22, 589�594
IMPORT NOTES: Special character found in field(Comments)
Proper citation: RRID:Addgene_100696 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector for second step assembly
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function and internal BsaI site generating a ATCC overhang upon cleavage. It is used in the first step BsmBI-based Golden Gate along with HC_Kan_conn_BsaI-1_p1 to flank the vector with BsaI stes. Vectors built in the first BsmBI-based Golden Gate reaction will be flanked by BsaI sites that are used in a second step assembly using as entry vector HC_RFP-4 (the part-entry vector for position 4). See manuscript for futher information
Proper citation: RRID:Addgene_100669 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector Q-W
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 16 and 23 of EMMA platform.
Proper citation: RRID:Addgene_100660 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector W-Y
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 22 and 25 of EMMA platform.
Proper citation: RRID:Addgene_100665 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector W-X
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 22 and 24 of EMMA platform.
Proper citation: RRID:Addgene_100666 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector T-V
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 19 and 22 of EMMA platform.
Proper citation: RRID:Addgene_100662 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector U-V
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 20 and 22 of EMMA platform.
Proper citation: RRID:Addgene_100663 Copy
Species: Homo sapiens
Genetic Insert: Kozak-ATG-MLS
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Kozak-ATG-MLS (Mitochondrial Localization Sequence)
Proper citation: RRID:Addgene_100676 Copy
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