Species: Homo sapiens
Genetic Insert: synuclein alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5900; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid encodes a novel 18-amino-acid protein tag - "NE", which can be detected by a specific mouse monoclonal antibody in Western blotting, immunopreciptation, and immunocytochemistry. (Source of antibody: http://www.versitech.hku.hk/reagents/ne/)
Proper citation: RRID:Addgene_102361 Copy
Species: Homo sapiens
Genetic Insert: LGALS3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA6-myc his version B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The his-tag and myc-tag are NOT in frame with the Gal3-mAzami green.
Proper citation: RRID:Addgene_102419 Copy
Species: Homo sapiens
Genetic Insert: Lipoprotein lipase
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102447 Copy
Species: Homo sapiens
Genetic Insert: MS2-P65-HSF1_T2A_Hygro
Vector Backbone Description: Backbone Size:3902; Vector Backbone:piggybac; Vector Types:Mammalian Expression, CRISPR, Other, piggybac transposon; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102559 Copy
Species: Homo sapiens
Genetic Insert: PTGS2
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102498 Copy
Species: Homo sapiens
Genetic Insert: MYC
Vector Backbone Description: Backbone Size:4319; Vector Backbone:pCMV-Tag-4A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102625 Copy
Species: Homo sapiens
Genetic Insert: STING
Vector Backbone Description: Vector Backbone:pMSCV-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102599 Copy
Species: Homo sapiens
Genetic Insert: Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1
Vector Backbone Description: Backbone Marker:Novagen.EMD Millipore; Backbone Size:3719; Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: This plasmid was specifically designed to allow high levels of RAD51 expression in E.coli along with the ability to incorporate non-natural amino acids using amber suppressor technology. It was used to incorporate an artificial amino acid to mimic tyrosine phosphorylation (published in Subramanyam S, et. al. (2016). Proceedings of the National Academy of Sciences 113(41):E6045-E6054).
Proper citation: RRID:Addgene_102562 Copy
Species: Homo sapiens
Genetic Insert: STING
Vector Backbone Description: Vector Backbone:pMSCV-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102600 Copy
Species: Homo sapiens
Genetic Insert: RPA1
Vector Backbone Description: Vector Backbone:pET11d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Note: The RPA2 gene in this plasmid is a variant that also contains a T88I mutation.
Proper citation: RRID:Addgene_102617 Copy
Species: Homo sapiens
Genetic Insert: RPA1
Vector Backbone Description: Vector Backbone:pET11d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102613 Copy
Species: Homo sapiens
Genetic Insert: APPL1
Vector Backbone Description: Backbone Size:4722; Vector Backbone:mCherry-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: *Note: Addgene's quality control sequencing finds additional residues KPNSAVDGTAGPGSTGSR appended to the C-terminus of the APPL1 protein. These additional residues are not thought to affect protein function.
Proper citation: RRID:Addgene_102784 Copy
Species: Homo sapiens
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Size:4731; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: mCherry-C2-Rab5a encodes wild-type human Rab5a,(NM_004162) as a fusion protein with mCherry. Rab5a was inserted between the Eco R1 and Sal 1 restriction sites. mCherry-C2 encodes a monomeric variant of DsRed. The backbone plasmid is pEGFP-C1 (Clontech). The C2 version of mCherry was created by insertion of a short piece of DNA between Xho 1 and EcoR1 restriction sites. In the resulting multiple cloning site, the Sac 1 restriction site has been eliminated and the Eco R1 restriction site is in frame.
Proper citation: RRID:Addgene_102785 Copy
Species: Homo sapiens
Genetic Insert: Zinc finger protein 57
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:NEB DHFR control plasmid; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102716 Copy
Species: Homo sapiens
Genetic Insert: EPHA3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1(+)/myc-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note last 10 amino acids in C-terminal are omitted in these constructs. These series of EPHA3 constructs carry missense mutations found in lung and colon cancer.
Proper citation: RRID:Addgene_102748 Copy
Species: Homo sapiens
Genetic Insert: EPHA3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1(+)/myc-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note last 10 amino acids in C-terminal are omitted in these constructs. These series of EPHA3 constructs carry missense mutations found in lung and colon cancer.
Proper citation: RRID:Addgene_102745 Copy
Species: Homo sapiens
Genetic Insert: EPHA3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1(+)/myc-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note last 10 amino acids in C-terminal are omitted in these constructs. These series of EPHA3 constructs carry missense mutations found in lung and colon cancer.
Proper citation: RRID:Addgene_102740 Copy
Species: Homo sapiens
Genetic Insert: EPHA3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1(+)/myc-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note last 10 amino acids in C-terminal are omitted in these constructs. These series of EPHA3 constructs carry missense mutations found in lung and colon cancer.
Proper citation: RRID:Addgene_102741 Copy
Species: Homo sapiens
Genetic Insert: EPHA3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1(+)/myc-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note last 10 amino acids in C-terminal are omitted in these constructs. These series of EPHA3 constructs carry missense mutations found in lung and colon cancer.
Proper citation: RRID:Addgene_102742 Copy
Species: Homo sapiens
Genetic Insert: EPHA3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1(+)/myc-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note last 10 amino acids in C-terminal are omitted in these constructs. These series of EPHA3 constructs carry missense mutations found in lung and colon cancer.
Proper citation: RRID:Addgene_102750 Copy
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