Searching the RRID Resource Information Network
Species: Homo sapiens
Genetic Insert: Barcoded piggyBac Puro SRT
Vector Backbone Description: Vector Backbone:PB-SRT-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_193162 Copy
Species: Homo sapiens
Genetic Insert: TRPM7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
References:
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23476 Copy
Species: Homo sapiens
Genetic Insert: MSH2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: A full-length clone of hMSH2 cDNA was cloned into the XbaI/XhoI site of pBluescript SK.
Proper citation: RRID:Addgene_16453 Copy
Species: Homo sapiens
Genetic Insert: human MSH2_G674A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4776; Vector Backbone:pFastBac 1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_134874 Copy
Species: Homo sapiens
Genetic Insert: YTHDC1
Vector Backbone Description: Vector Backbone:modified from Addgene 61425; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177129 Copy
Species: Homo sapiens
Genetic Insert: BAF (BANF1)
Vector Backbone Description: Backbone Size:5517; Vector Backbone:custom bacterial expression vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_101779 Copy
Species: Homo sapiens
Genetic Insert: CETN2 Homology Arms with mTagRFPT-linker
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Other, Donor Template; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human CETN2 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_101781 Copy
Species: Homo sapiens
Genetic Insert: TUBA1B Homology Arms with mTagRFPT-linker
Vector Backbone Description: Backbone Marker:New England BioLabs; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Other, Donor Template; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human TUBA1B in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GGSGGS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_101785 Copy
Species: Homo sapiens
Genetic Insert: SRY-box 2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101761 Copy
Species: Homo sapiens
Genetic Insert: transforming growth factor beta 1 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101762 Copy
Species: Homo sapiens
Genetic Insert: SMAD2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101763 Copy
Species: Homo sapiens
Genetic Insert: Flag-BPTF
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: BPTF insert contains amino acid residues 140-2903 with C1746R, T1752A, D1852N variants compared to the NCBI reference [NM_004459.6].
Proper citation: RRID:Addgene_102242 Copy
Species: Homo sapiens
Genetic Insert: LAMP-mCherry-CRY2
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102249 Copy
Species: Homo sapiens
Genetic Insert: METTL14
Vector Backbone Description: Vector Backbone:pFBOH-LIC notag; Vector Types:Other, Baculovirus expression; Bacterial Resistance:Ampicillin
References:
Comments: N terminal tag: none. SGC Clone Sample ID: METTL14:JMC097-G06:C232534. SGC or PDG link: http://www.thesgc.org/structures/5TEY/
Proper citation: RRID:Addgene_101891 Copy
Species: Homo sapiens
Genetic Insert: METTL3
Vector Backbone Description: Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 62304); Vector Backbone:pFBOH-MHL; Vector Types:Other, Baculovirus expression; Bacterial Resistance:Ampicillin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: METTL3:JMC094-C09:C231635. SGC or PDG link: http://www.thesgc.org/structures/5TEY/
Proper citation: RRID:Addgene_101892 Copy
Species: Homo sapiens
Genetic Insert: USP15_SART3
Vector Backbone Description: Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: SART3|USP15:YTC044-A09:C237276. SGC or PDG link: http://www.thesgc.org/structures/5JJW/
Proper citation: RRID:Addgene_101880 Copy
Species: Homo sapiens
Genetic Insert: SETDB1
Vector Backbone Description: Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: SETDB1:APC043-C07:C27642.
SGC or PDG links:
5KCH http://www.thesgc.org/structures/5KCH/
6AU2 http://www.thesgc.org/structures/6AU2/
6AU3 http://www.thesgc.org/structures/6AU3/
6BPI http://www.thesgc.org/structures/6BPI/
5KCO http://www.thesgc.org/structures/5KCO/
5KE2 http://www.thesgc.org/structures/5KE2/
5KE3 http://www.thesgc.org/structures/5KE3/
5KH6 http://www.thesgc.org/structures/5KH6/
Proper citation: RRID:Addgene_101881 Copy
Species: Homo sapiens
Genetic Insert: SETD8
Vector Backbone Description: Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: SETD8:PBC001-G01:C233009. SGC or PDG link:
http://www.thesgc.org/structures/5T5G/
http://www.thesgc.org/structures/5TH7/
Proper citation: RRID:Addgene_101886 Copy
Species: Homo sapiens
Genetic Insert: PREB
Vector Backbone Description: Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 62304); Vector Backbone:pFBOH-MHL; Vector Types:Other, Baculovirus expression; Bacterial Resistance:Ampicillin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: PREB:YTC012-F10:C229890. SGC or PDG link: http://www.thesgc.org/structures/5TF2/
Proper citation: RRID:Addgene_101889 Copy
Species: Homo sapiens
Genetic Insert: Rab interacting lysosomal protein
Vector Backbone Description: Backbone Size:4858; Vector Backbone:pTre2-Bla; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102425 Copy
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