Searching the RRID Resource Information Network
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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PB-SRT-Puro_BC20 Resource Report Resource Website |
RRID:Addgene_193162 | Barcoded piggyBac Puro SRT | Homo sapiens | Ampicillin | PMID:36062164 | Vector Backbone:PB-SRT-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Barcode: TCAG | 2022-12-07 12:04:30 | 0 | |
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pDONR223-TRPM7 Resource Report Resource Website |
RRID:Addgene_23476 | TRPM7 | Homo sapiens | Spectinomycin | PMID:21107320 | The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately. | Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin | Insert encodes NCBI: NR_149154.2 bases 265 to 1468, and bases 5532 to 5660 | 2022-12-11 12:04:19 | 0 |
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hMSH2 Clone E3 (full-length) Resource Report Resource Website 1+ mentions |
RRID:Addgene_16453 | MSH2 | Homo sapiens | Ampicillin | PMID:8261515 | A full-length clone of hMSH2 cDNA was cloned into the XbaI/XhoI site of pBluescript SK. | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-11-28 12:02:58 | 1 | |
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pFB1_hMSH2_G674A Resource Report Resource Website |
RRID:Addgene_134874 | human MSH2_G674A | Homo sapiens | Ampicillin | PMID:21329650 | Backbone Marker:Invitrogen; Backbone Size:4776; Vector Backbone:pFastBac 1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | Changed Glycine to Alanine at aa#674 | 2022-11-28 12:01:52 | 0 | |
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pLenti-Ef1a-mClover-YTHDC1-T2A-BSD-siRNA-Resistant Resource Report Resource Website |
RRID:Addgene_177129 | YTHDC1 | Homo sapiens | Ampicillin | PMID:34375583 | Vector Backbone:modified from Addgene 61425; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | siRNA resistant silent mutations | 2022-12-15 12:04:05 | 0 | |
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H14-MBP-SUMO-Cys-Linker-BAF_G47E Resource Report Resource Website |
RRID:Addgene_101779 | BAF (BANF1) | Homo sapiens | Kanamycin | PMID:28841419 | Backbone Size:5517; Vector Backbone:custom bacterial expression vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | G47E mutation | 2022-04-22 03:15:09 | 0 | |
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AICSDP-22:CETN2-mTagRFP-T Resource Report Resource Website |
RRID:Addgene_101781 | CETN2 Homology Arms with mTagRFPT-linker | Homo sapiens | Ampicillin | PMID: | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human CETN2 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Other, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used | 2022-04-22 03:15:09 | 0 |
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AICSDP-28:TUBA1B-mTagRFP-T Resource Report Resource Website |
RRID:Addgene_101785 | TUBA1B Homology Arms with mTagRFPT-linker | Homo sapiens | Ampicillin | PMID: | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human TUBA1B in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GGSGGS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Backbone Marker:New England BioLabs; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Other, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used | 2022-04-22 03:15:10 | 0 |
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pGL3-Sox2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101761 | SRY-box 2 promoter | Homo sapiens | Ampicillin | PMID:29593326 | Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:09 | 1 | ||
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pGL3-TGFb1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101762 | transforming growth factor beta 1 promoter | Homo sapiens | Ampicillin | PMID:29593326 | Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:09 | 2 | ||
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CS2 Flag-Smad2 (3A) Resource Report Resource Website |
RRID:Addgene_101763 | SMAD2 | Homo sapiens | Ampicillin | PMID:29593326 | Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Three Ser to Ala mutations in the phosphorylation sites (S464A, S465A, S467A) | 2022-04-22 03:15:09 | 0 | |
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pFastBac1_FLAG-BPTF Resource Report Resource Website |
RRID:Addgene_102242 | Flag-BPTF | Homo sapiens | Ampicillin | PMID: | BPTF insert contains amino acid residues 140-2903 with C1746R, T1752A, D1852N variants compared to the NCBI reference [NM_004459.6]. | Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:12 | 0 | |
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LAMP-mCherry-CRY2 Resource Report Resource Website |
RRID:Addgene_102249 | LAMP-mCherry-CRY2 | Homo sapiens | Kanamycin | PMID:25963241 | Backbone Size:4000; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:12 | 0 | ||
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5TEY (METTL14) Resource Report Resource Website |
RRID:Addgene_101891 | METTL14 | Homo sapiens | Ampicillin | PMID: | N terminal tag: none. SGC Clone Sample ID: METTL14:JMC097-G06:C232534. SGC or PDG link: http://www.thesgc.org/structures/5TEY/ | Vector Backbone:pFBOH-LIC notag; Vector Types:Other, Baculovirus expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:12 | 0 | |
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5TEY (METTL3) Resource Report Resource Website 1+ mentions |
RRID:Addgene_101892 | METTL3 | Homo sapiens | Ampicillin | PMID: | N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: METTL3:JMC094-C09:C231635. SGC or PDG link: http://www.thesgc.org/structures/5TEY/ | Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 62304); Vector Backbone:pFBOH-MHL; Vector Types:Other, Baculovirus expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:12 | 1 | |
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5JJW (USP15_SART3) Resource Report Resource Website |
RRID:Addgene_101880 | USP15_SART3 | Homo sapiens | Kanamycin | PMID: | N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: SART3|USP15:YTC044-A09:C237276. SGC or PDG link: http://www.thesgc.org/structures/5JJW/ | Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:11 | 0 | |
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5KCH (SETDB1) Resource Report Resource Website |
RRID:Addgene_101881 | SETDB1 | Homo sapiens | Kanamycin | PMID: | N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: SETDB1:APC043-C07:C27642. SGC or PDG links: 5KCH http://www.thesgc.org/structures/5KCH/ 6AU2 http://www.thesgc.org/structures/6AU2/ 6AU3 http://www.thesgc.org/structures/6AU3/ 6BPI http://www.thesgc.org/structures/6BPI/ 5KCO http://www.thesgc.org/structures/5KCO/ 5KE2 http://www.thesgc.org/structures/5KE2/ 5KE3 http://www.thesgc.org/structures/5KE3/ 5KH6 http://www.thesgc.org/structures/5KH6/ | Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:11 | 0 | |
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5T5G (SETD8) Resource Report Resource Website |
RRID:Addgene_101886 | SETD8 | Homo sapiens | Kanamycin | PMID: | N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: SETD8:PBC001-G01:C233009. SGC or PDG link: http://www.thesgc.org/structures/5T5G/ http://www.thesgc.org/structures/5TH7/ | Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:11 | 0 | |
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5TF2 (PREB) Resource Report Resource Website |
RRID:Addgene_101889 | PREB | Homo sapiens | Ampicillin | PMID: | N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: PREB:YTC012-F10:C229890. SGC or PDG link: http://www.thesgc.org/structures/5TF2/ | Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 62304); Vector Backbone:pFBOH-MHL; Vector Types:Other, Baculovirus expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:12 | 0 | |
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pTRE2-Bla(HA–RILP) Resource Report Resource Website |
RRID:Addgene_102425 | Rab interacting lysosomal protein | Homo sapiens | Ampicillin | PMID:27791088 | Backbone Size:4858; Vector Backbone:pTre2-Bla; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:15 | 0 |
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