Searching the RRID Resource Information Network
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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ML26 Resource Report Resource Website |
RRID:Addgene_61917 | none | None | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG argH Precursor strain = ML23 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:38 | 0 | ||
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ML31 Resource Report Resource Website |
RRID:Addgene_61918 | none | None | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG argH metA Precursor strain = ML26 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg, Met *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:38 | 0 | ||
|
ML21 Resource Report Resource Website |
RRID:Addgene_61914 | none | None | PMID:21925267 | Genotype = tyrA hisG Precursor strain = ML14 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Tyr), His Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:38 | 0 | ||
|
DH10B-ALT Resource Report Resource Website |
RRID:Addgene_61151 | None | PMID:23479654 | In addition to the wt copy of lacI, another copy of lacI driven by PIq is integrated at the attB Contains another copy of araC driven by a constitutive promoter in addition to wt araC. | Vector Backbone:NA; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:36 | 0 | |||
|
ML43 Resource Report Resource Website |
RRID:Addgene_61922 | none | None | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG argH metA lysA thrC asnA asnB Precursor strain = ML42 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg, Met, Lys, Thr, Asn *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:38 | 0 | ||
|
MS1 Resource Report Resource Website |
RRID:Addgene_61960 | none | None | PMID:26577727 | Genotype = cysE hisG Precursor strain = YM138 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = Cys, His Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:38 | 0 | ||
|
RF1 Resource Report Resource Website |
RRID:Addgene_61962 | none | None | PMID:26577727 | Genotype = glyA Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Gly Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:39 | 0 | ||
|
HL 1951 Resource Report Resource Website |
RRID:Addgene_61162 | See comments | None | PMID:22833608 | MG1655 + PLlacO-1::T710::yfp intergrated at galK | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-14 01:07:36 | 0 | ||
|
HL 1852 Resource Report Resource Website |
RRID:Addgene_61161 | See comments | None | PMID:22833608 | HL1745 + PLlacO-1::T710::yfp intergrated at galK | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-14 01:07:36 | 0 | ||
|
HL 5222 Resource Report Resource Website |
RRID:Addgene_61166 | See comments | None | PMID:22833608 | MG1655 + PLlacO-1::T710::yfp intergrated at yjiP + ∆lacI | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-14 01:07:36 | 0 | ||
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HL 5221 Resource Report Resource Website |
RRID:Addgene_61165 | See comments | None | PMID:22833608 | MG1655 + PLlacO-1::T710::yfp intergrated at yfjV + ∆lacI | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-14 01:07:36 | 0 | ||
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S4197 Resource Report Resource Website |
RRID:Addgene_200839 | Genotype: MG1655 rph+, ilvG+, ΔlacZ | Other | None | PMID:20952573 | Corresponding wild-type strain (rapZ+, glmZ+, glmY+) for strain Z956 (Addgene Bacterial strain #200838). | Vector Backbone:n/a; Vector Types:Other, This is a strain, not a plasmid; Bacterial Resistance:None | 2023-07-27 01:04:30 | 0 | |
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HL 6703 Resource Report Resource Website |
RRID:Addgene_69778 | None | PMID:27084942 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-09-15 01:13:30 | 0 | ||||
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HL 6686 Resource Report Resource Website |
RRID:Addgene_69775 | None | PMID:27084942 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-09-15 01:13:30 | 0 | ||||
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HL 3500 Resource Report Resource Website |
RRID:Addgene_69774 | None | PMID:27084942 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-09-15 01:13:30 | 0 | ||||
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HL 6778 Resource Report Resource Website |
RRID:Addgene_69782 | None | PMID:27084942 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-09-15 01:13:30 | 0 | ||||
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HL 6777 Resource Report Resource Website |
RRID:Addgene_69781 | None | PMID:27084942 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-09-15 01:13:30 | 0 | ||||
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LC-E18 Resource Report Resource Website |
RRID:Addgene_115924 | none | None | PMID:29765036 | E. coli K12 MG1655 genotype: F- λ- ilvG- rfb-50 rph-1 Integration at lambda attB: pOSIP-KL-sulA-GFP Integration at HK022 attB: pOSIP-KO-RBS2-dCas9 SulA-GFP acts as an SOS response reporter. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-09-15 01:02:23 | 0 | ||
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E. coli BL21 (DE3) ΔEntD Resource Report Resource Website |
RRID:Addgene_192874 | The strain has a 535 nt deletion between nt 15 and nt 551 in the EntD gene. | Other | None | PMID:16709676 | EntD, a 4'-phosphopantetheinyl transferase, is required for native enterobactin synthesis. This strain allows for expression of nonribosomal peptide synthetase (NRPS) carrier proteins that do not harbor a phosphopantetheinyl group. | Vector Backbone:n/a; Vector Types:Other, This is a strain, not a plasmid; Bacterial Resistance:None | 2023-09-09 01:09:18 | 0 | |
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B-95.ΔA Resource Report Resource Website |
RRID:Addgene_197933 | RNA polymerase gene (T7 phage)/chromosome | None | PMID:25982672 | Genotype: The same as BL21(DE3) except for the additional mutations at 95 UAG codons and disruption of the prfA gene | Vector Backbone:BL21(DE3); Vector Types:; Bacterial Resistance:None | 2023-11-29 12:04:49 | 0 |
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