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  • Bacterial Resistance:chloramphenicol and ampicillin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pDEST-pSP172BSSPE-pFOGc::RfA
 
Resource Report
Resource Website
RRID:Addgene_186397 Chloramphenicol and Ampicillin PMID:17878951 Gateway destination vector for electroporation experiments. Backbone was modified by insertion of tag at SwaI restriction site Backbone Marker:PMID: 17878951; Backbone Size:4650; Vector Backbone:pDEST-pSP172BSSPE-Swa1::RfA; Vector Types:Other, Gateway destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-10-07 04:38:34 0
pDEST-pSP172BSSPE-Swa1::RfA
 
Resource Report
Resource Website
RRID:Addgene_186396 Chloramphenicol and Ampicillin PMID:17878951 Gateway destination vector for electroporation experiments. The electroporation vector pSP72-1.27 was modified to give rise to pSP72BSSPE by replacing the NLS-LacZ reporter gene, cloned between BamH1 and EcoR1 by a BamH1-Swa1-Stu1-Pme1-EcoRV-EcoR1 polylinker (GGATCCATTTAAATAGGCCTTTGTTTAAACTTAGATATCGGAATTC). Backbone Marker:PMID: 9043074; Backbone Size:4650; Vector Backbone:Ascidian electroporation vector pSP72-1.27; Vector Types:Other, Gateway destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-10-07 04:51:27 0
pDEST-Spe4-RfA-Snaptag-stop
 
Resource Report
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RRID:Addgene_186395 Chloramphenicol and Ampicillin PMID: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of C terminal Snap-tag tag at EcoR V/Avr II restriction site Backbone Size:5412; Vector Backbone:pDEST-Spe4-RfA; Vector Types:Other, Gateway destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-10-07 04:51:29 0
pDEST-pSP172BSSPE-pFOGc::venus-RfA
 
Resource Report
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RRID:Addgene_186400 Chloramphenicol and Ampicillin PMID:17878951 Gateway destination vector for electroporation experiments. Backbone was modified by insertion of N terminal Venus tag at SwaI restriction site Backbone Marker:PMID: 17878951; Backbone Size:5385; Vector Backbone:pDEST-pSP172BSSPE-Swa1::venus-RfA; Vector Types:Other, Gateway destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-10-07 04:51:28 0
pDEST-Spe4-Kozak-mCherry-RfA
 
Resource Report
Resource Website
RRID:Addgene_186378 Chloramphenicol and Ampicillin PMID: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of N terminal mCherry tag at Bgl II/Stu I restriction site Backbone Size:5589; Vector Backbone:pDEST-Spe4-RfA; Vector Types:Other, Gateway destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-10-07 04:51:30 0
pDEST-Spe4-RfA
 
Resource Report
Resource Website
RRID:Addgene_186375 Chloramphenicol and Ampicillin PMID: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. This plasmid was obtained by gene synthesis from pDEST-Spe3-RfA by addition of a BglII-ZraI-AatII-StuI-SnaBI (agatctgacgtcaggccttacgta) polylinker before the attR1 sequence and an EcoRV-SacII-AfeI-BstEII-AvrII (gatatcgcggccgcggaagcgctggttacctagg) polylinker after the attR2 sequence. Backbone Marker:PMID: 17878951; Backbone Size:4888; Vector Backbone:pDEST-Spe3-RfA; Vector Types:Other, Gateway destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-10-07 04:51:29 0
pDEST-Spe3-RfA-HA
 
Resource Report
Resource Website
RRID:Addgene_186374 Chloramphenicol and Ampicillin PMID:17878951 Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of C terminal HA tag at EcoR V restriction site Backbone Marker:PMID: 17878951; Backbone Size:4915; Vector Backbone:pDEST-Spe3-RfA; Vector Types:Other, Gateway destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-10-07 04:38:34 0
pDEST-Spe3-HA-RfA
 
Resource Report
Resource Website
RRID:Addgene_186373 Chloramphenicol and Ampicillin PMID:17878951 Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of N terminal HA tag at Stu I restriction site Backbone Marker:PMID: 17878951; Backbone Size:4926; Vector Backbone:pDEST-Spe3-RfA; Vector Types:Other, Gateway destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-10-07 04:38:34 0
pDEST-periHisMBP
 
Resource Report
Resource Website
RRID:Addgene_11086 Chloramphenicol and Ampicillin PMID:16322578 The MBP ORF can be sequenced using 5'-GAG CGG ATA ACA ATT TCA CAC AGG-3' as a tac promoter sequencing primer (in the forward direction). To sequence inserts fused to the C-terminus of MBP, the primers are 5'-GAT GAA GCC CTG AAA GAC GCG CAG-3' (forward) and 5'-GCA AGG CGA TTA AGT TGG GTA ACG C-3' (reverse). Backbone Marker:New England Biolabs; Backbone Size:6000; Vector Backbone:pMal-C2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-04-22 03:17:32 0
pUC57-R1R4
 
Resource Report
Resource Website
RRID:Addgene_105110 Chloramphenicol and Ampicillin PMID:23066669 Vector Backbone:pUC57-R1R4; Vector Types:Other, Other; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-04-22 03:15:54 0
pXN21-Dest
 
Resource Report
Resource Website
RRID:Addgene_105086 Chloramphenicol and Ampicillin PMID:18428659 Vector Backbone:pXN21-Dest; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-04-22 03:15:54 0
pDEST-actc1b-mKate2-ccdb
 
Resource Report
Resource Website
RRID:Addgene_109732 Chloramphenicol and Ampicillin PMID:32709891 Vector Backbone:pDEST-Tol2-pA2; Vector Types:Other, Zebrafish plasmids; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-04-22 03:17:09 0
pBXNPH3
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_110098 Chloramphenicol and Ampicillin PMID:29792401 Vector Backbone:pBXNPH3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-04-22 03:17:17 1
pBXNPHM3
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_110099 Chloramphenicol and Ampicillin PMID:29792401 Vector Backbone:pBXNPHM3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-04-22 03:17:17 1
pFRT-TO-DEST
 
Resource Report
Resource Website
RRID:Addgene_106348 Chloramphenicol and Ampicillin PMID:28297718 Vector Backbone:pFRT-EGFP-DEST; Vector Types:Mammalian Expression, Other, Gateway entry vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-04-22 03:16:16 0
Cas9 gateway compatible
 
Resource Report
Resource Website
RRID:Addgene_128321 Chloramphenicol and Ampicillin PMID: Backbone Size:11000; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-04-22 03:23:15 0
pGAN147
 
Resource Report
Resource Website
RRID:Addgene_129202 Chloramphenicol and Ampicillin PMID:29056345 This is the empty basic yTRAP sensor plasmid. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pGAN147. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the HO locus, and can be selected for using nourseothricin. Backbone Marker:Dirk Landgraf; Backbone Size:5056; Vector Backbone:pDML112; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-04-22 03:23:35 0
pMW#2
 
Resource Report
Resource Website
RRID:Addgene_13349 HIS3 reporter Chloramphenicol and Ampicillin PMID:16777607 Please note that the ccdB cassette in the author's map (click on 'View map') is shown in the reverse orientation. Y1H Destination vector that can be used in conventional Gateway LR cloning reactions. This vector contains a Gateway cassette with AttR4 and AttL1 recombination sites and a HIS3 reporter gene. For more information and protocols, see Deplancke et al. 2004 Genome Res 14(10B):2093 and Deplancke et al. 2006 CSH Protocols. Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pDEST6; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-04-22 03:24:54 0
pAW
 
Resource Report
Resource Website
RRID:Addgene_133893 Chloramphenicol and Ampicillin PMID:30217970 Backbone Size:9709; Vector Backbone:pAW; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-04-22 03:25:00 0
pGL4.23-SCP1-ccdB
 
Resource Report
Resource Website
RRID:Addgene_131628 Chloramphenicol and Ampicillin PMID:29151363 Backbone Marker:Promega; Backbone Size:5813; Vector Backbone:pGL4.23; Vector Types:Mammalian Expression, Bacterial Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin 2022-04-22 03:24:12 0

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