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http://www.addgene.org/25867

Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gz alpha residues (EYNLV to YIGLC). Works the same as qi5 and is the least popular since no one knows what Gz alpha really does in nature. Since qz5 is not sensitive to pertussis toxin, qz5 may be the only G protein activated by a Gi-coupled receptor in cells treated with pertussis toxin. This trick can be experimentally useful in settings where you want experimental control of the exact G protein and the receptor that is activated. It is theoretically possible that this construct will work better than the other constructs for particular receptors, but the Conklin lab has not seen this happen yet. Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual.

Proper citation: RRID:Addgene_25867 Copy   

•   Source: Addgene

http://www.addgene.org/11869

Species: Homo sapiens
Genetic Insert: Bystin
Vector Backbone Description: Backbone Size:4033; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_11869 Copy   

•   Source: Addgene

http://www.addgene.org/182547

Species:
Genetic Insert: H5_Vietnam_1203_04_Y161A
Vector Backbone Description: Backbone Size:3400; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_182547 Copy   

•   Source: Addgene

http://www.addgene.org/107582

Species: Synthetic
Genetic Insert: GFP+
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:5796; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology, Other, E. coli and Bacillus shuttle vector; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_107582 Copy   

•   Source: Addgene

http://www.addgene.org/107578

Species: Synthetic
Genetic Insert: GFP+
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:6336; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_107578 Copy   

•   Source: Addgene

http://www.addgene.org/175020

Species: Synthetic
Genetic Insert: D/bovine/Nebraska/9-5/2012
Vector Backbone Description: Backbone Marker:-; Backbone Size:3500; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_175020 Copy   

•   Source: Addgene

http://www.addgene.org/175019

Species: Synthetic
Genetic Insert: D/bovine/Nebraska/9-5/2012
Vector Backbone Description: Backbone Marker:-; Backbone Size:3500; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_175019 Copy   

•   Source: Addgene

http://www.addgene.org/157933

Species: Synthetic
Genetic Insert: A/PuertoRico/8/34 hemagglutinin
Vector Backbone Description: Backbone Marker:-; Backbone Size:3500; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_157933 Copy   

•   Source: Addgene

http://www.addgene.org/158742

Species: Other
Genetic Insert: Neuraminidase A/NL/354/16 H3N2
Vector Backbone Description: Backbone Marker:-; Backbone Size:4800; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_158742 Copy   

•   Source: Addgene

http://www.addgene.org/158740

Species:
Genetic Insert: Hemagglutinin A/MI/45/15 H1N1
Vector Backbone Description: Backbone Marker:-; Backbone Size:5400; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_158740 Copy   

•   Source: Addgene

http://www.addgene.org/21900

Species: Mus musculus
Genetic Insert: CHOP10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: Addgene sequencing results show that only half of the leurin zipper is deleted, protein ends at amino acid 133. Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence. Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure.

Proper citation: RRID:Addgene_21900 Copy   

•   Source: Addgene

http://www.addgene.org/200993

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:6393; Vector Backbone:pTn7-SCOUT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: Ec100D pir-116 genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell. This strain is naturally resistant to streptomycin

Proper citation: RRID:Addgene_200993 Copy   

•   Source: Addgene