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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
qz5
 
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RRID:Addgene_25867 Gq alpha Mus musculus Ampicillin and Tetracycline PMID:8387644 This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gz alpha residues (EYNLV to YIGLC). Works the same as qi5 and is the least popular since no one knows what Gz alpha really does in nature. Since qz5 is not sensitive to pertussis toxin, qz5 may be the only G protein activated by a Gi-coupled receptor in cells treated with pertussis toxin. This trick can be experimentally useful in settings where you want experimental control of the exact G protein and the receptor that is activated. It is theoretically possible that this construct will work better than the other constructs for particular receptors, but the Conklin lab has not seen this happen yet. Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline Replacement of the five carboxyl-terminal amino acids of Gq alpha (EYNLV) with those of Gz alpha(YIGLC) 2022-09-09 01:02:52 0
pcDNA1-Bystin
 
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RRID:Addgene_11869 Bystin Homo sapiens Ampicillin and Tetracycline PMID:9560222 Backbone Size:4033; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline 2022-04-22 03:19:58 0
pCD5-H5_Vietnam_1203/04_Y161A_sfGFP_ST
 
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RRID:Addgene_182547 H5_Vietnam_1203_04_Y161A Ampicillin and Tetracycline PMID:31189111 Backbone Size:3400; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline Y161A mutant that confers exclusive NeuAc to NeuGc specificity 2023-09-15 01:07:51 0
pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015
 
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RRID:Addgene_107582 GFP+ Synthetic Ampicillin and Tetracycline PMID:29666238 Backbone Marker:N/A; Backbone Size:5796; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology, Other, E. coli and Bacillus shuttle vector; Bacterial Resistance:Ampicillin and Tetracycline F64L/ S65T/ Q80R/ F99S/ M153T/ V163A 2023-09-15 01:01:03 0
pTU1-A-fbp_RiboJ_GFP+_MGApt_Bba_B0015
 
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RRID:Addgene_107578 GFP+ Synthetic Ampicillin and Tetracycline PMID:29666238 Backbone Marker:N/A; Backbone Size:6336; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Tetracycline F64L/ S65T/ Q80R/ F99S/ M153T/ V163A 2023-09-15 01:01:03 0
pCD5-D/bovine/Nebraska/9-5/2012-HEFs57aED-GCN4-sfGFP-ST
 
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RRID:Addgene_175020 D/bovine/Nebraska/9-5/2012 Synthetic Ampicillin and Tetracycline PMID:33807137 Backbone Marker:-; Backbone Size:3500; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline s57a esterase knock out mutant 2023-09-15 01:06:53 0
pCD5-D/bovine/Nebraska/9-5/2012-HEFwtED-GCN4-sfGFP-ST
 
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RRID:Addgene_175019 D/bovine/Nebraska/9-5/2012 Synthetic Ampicillin and Tetracycline PMID:33807137 Backbone Marker:-; Backbone Size:3500; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline 2023-09-15 01:06:53 0
pCD5-PR8-TEV-sfGFP-twinStrep
 
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RRID:Addgene_157933 A/PuertoRico/8/34 hemagglutinin Synthetic Ampicillin and Tetracycline PMID:30597163 Backbone Marker:-; Backbone Size:3500; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline E190D 2023-09-15 01:05:12 0
pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL16
 
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RRID:Addgene_158742 Neuraminidase A/NL/354/16 H3N2 Other Ampicillin and Tetracycline PMID:32710576 Backbone Marker:-; Backbone Size:4800; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline AA61-469 2023-09-15 01:05:16 0
pCD5-H3CH13-CO-GCN4-TEV-sfGFP-TS
 
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RRID:Addgene_158740 Hemagglutinin A/MI/45/15 H1N1 Ampicillin and Tetracycline PMID:32710576 Backbone Marker:-; Backbone Size:5400; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline AA61-469 2023-09-15 01:05:16 0
mCHOP10(dLZ).pCDNA1
 
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RRID:Addgene_21900 CHOP10 Mus musculus Ampicillin and Tetracycline PMID:1547942 Addgene sequencing results show that only half of the leurin zipper is deleted, protein ends at amino acid 133. Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence. Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure. Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline The coding region of the murine CHOP-10 cDNA was interrupted at the unique Nhe1 site, making delta leucine zipper 2024-02-21 12:06:18 0
pTn7-SCOUT13
 
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RRID:Addgene_200993 Ampicillin and Tetracycline PMID:38715147 Ec100D pir-116 genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell. This strain is naturally resistant to streptomycin Backbone Marker:Philip Poole lab; Backbone Size:6393; Vector Backbone:pTn7-SCOUT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline 2024-08-01 01:05:51 0

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