| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
qz5 Resource Report Resource Website |
RRID:Addgene_25867 | Gq alpha | Mus musculus | Ampicillin and Tetracycline | PMID:8387644 | This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gz alpha residues (EYNLV to YIGLC). Works the same as qi5 and is the least popular since no one knows what Gz alpha really does in nature. Since qz5 is not sensitive to pertussis toxin, qz5 may be the only G protein activated by a Gi-coupled receptor in cells treated with pertussis toxin. This trick can be experimentally useful in settings where you want experimental control of the exact G protein and the receptor that is activated. It is theoretically possible that this construct will work better than the other constructs for particular receptors, but the Conklin lab has not seen this happen yet. Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. | Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | Replacement of the five carboxyl-terminal amino acids of Gq alpha (EYNLV) with those of Gz alpha(YIGLC) | 2022-09-09 01:02:52 | 0 |
|
pcDNA1-Bystin Resource Report Resource Website |
RRID:Addgene_11869 | Bystin | Homo sapiens | Ampicillin and Tetracycline | PMID:9560222 | Backbone Size:4033; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | 2022-04-22 03:19:58 | 0 | ||
|
pCD5-H5_Vietnam_1203/04_Y161A_sfGFP_ST Resource Report Resource Website |
RRID:Addgene_182547 | H5_Vietnam_1203_04_Y161A | Ampicillin and Tetracycline | PMID:31189111 | Backbone Size:3400; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | Y161A mutant that confers exclusive NeuAc to NeuGc specificity | 2023-09-15 01:07:51 | 0 | ||
|
pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015 Resource Report Resource Website |
RRID:Addgene_107582 | GFP+ | Synthetic | Ampicillin and Tetracycline | PMID:29666238 | Backbone Marker:N/A; Backbone Size:5796; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology, Other, E. coli and Bacillus shuttle vector; Bacterial Resistance:Ampicillin and Tetracycline | F64L/ S65T/ Q80R/ F99S/ M153T/ V163A | 2023-09-15 01:01:03 | 0 | |
|
pTU1-A-fbp_RiboJ_GFP+_MGApt_Bba_B0015 Resource Report Resource Website |
RRID:Addgene_107578 | GFP+ | Synthetic | Ampicillin and Tetracycline | PMID:29666238 | Backbone Marker:N/A; Backbone Size:6336; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Tetracycline | F64L/ S65T/ Q80R/ F99S/ M153T/ V163A | 2023-09-15 01:01:03 | 0 | |
|
pCD5-D/bovine/Nebraska/9-5/2012-HEFs57aED-GCN4-sfGFP-ST Resource Report Resource Website |
RRID:Addgene_175020 | D/bovine/Nebraska/9-5/2012 | Synthetic | Ampicillin and Tetracycline | PMID:33807137 | Backbone Marker:-; Backbone Size:3500; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | s57a esterase knock out mutant | 2023-09-15 01:06:53 | 0 | |
|
pCD5-D/bovine/Nebraska/9-5/2012-HEFwtED-GCN4-sfGFP-ST Resource Report Resource Website |
RRID:Addgene_175019 | D/bovine/Nebraska/9-5/2012 | Synthetic | Ampicillin and Tetracycline | PMID:33807137 | Backbone Marker:-; Backbone Size:3500; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | 2023-09-15 01:06:53 | 0 | ||
|
pCD5-PR8-TEV-sfGFP-twinStrep Resource Report Resource Website |
RRID:Addgene_157933 | A/PuertoRico/8/34 hemagglutinin | Synthetic | Ampicillin and Tetracycline | PMID:30597163 | Backbone Marker:-; Backbone Size:3500; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | E190D | 2023-09-15 01:05:12 | 0 | |
|
pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL16 Resource Report Resource Website |
RRID:Addgene_158742 | Neuraminidase A/NL/354/16 H3N2 | Other | Ampicillin and Tetracycline | PMID:32710576 | Backbone Marker:-; Backbone Size:4800; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | AA61-469 | 2023-09-15 01:05:16 | 0 | |
|
pCD5-H3CH13-CO-GCN4-TEV-sfGFP-TS Resource Report Resource Website |
RRID:Addgene_158740 | Hemagglutinin A/MI/45/15 H1N1 | Ampicillin and Tetracycline | PMID:32710576 | Backbone Marker:-; Backbone Size:5400; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | AA61-469 | 2023-09-15 01:05:16 | 0 | ||
|
mCHOP10(dLZ).pCDNA1 Resource Report Resource Website |
RRID:Addgene_21900 | CHOP10 | Mus musculus | Ampicillin and Tetracycline | PMID:1547942 | Addgene sequencing results show that only half of the leurin zipper is deleted, protein ends at amino acid 133. Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence. Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure. | Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | The coding region of the murine CHOP-10 cDNA was interrupted at the unique Nhe1 site, making delta leucine zipper | 2024-02-21 12:06:18 | 0 |
|
pTn7-SCOUT13 Resource Report Resource Website |
RRID:Addgene_200993 | Ampicillin and Tetracycline | PMID:38715147 | Ec100D pir-116 genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell. This strain is naturally resistant to streptomycin | Backbone Marker:Philip Poole lab; Backbone Size:6393; Vector Backbone:pTn7-SCOUT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline | 2024-08-01 01:05:51 | 0 |
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