Species: Homo sapiens
Genetic Insert: mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS
Vector Backbone Description: Backbone Size:5472; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192445 Copy
Species: Homo sapiens
Genetic Insert: truncated ADAM metallopeptidase domain 10
Vector Backbone Description: Backbone Marker:Customized backbone from Fu lab ; Vector Backbone:pSCM167-Venus (Fu lab); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note: Plasmid contains a Y316H mutation in hADAM10 compared to the NCBI reference sequence NP_001101.1. This mutation was present in the original DNA that was was PCR amplified from human placenta cDNA and is not known to affect plasmid function.
Proper citation: RRID:Addgene_189555 Copy
Species: Mus musculus
Genetic Insert: Gasdermin D
Vector Backbone Description: Vector Backbone:pCAG-FOS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Backbone is from Dr. Eiji Morita
Proper citation: RRID:Addgene_111565 Copy
Species: Homo sapiens
Genetic Insert: truncated secreted frizzled related protein 1
Vector Backbone Description: Backbone Marker:Customized backbone from Fu lab ; Vector Backbone:pSCM167-Venus (Fu lab); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_189550 Copy
Species: Mus musculus
Genetic Insert: RIPK3
Vector Backbone Description: Backbone Marker:GE Dharmacon; Backbone Size:12300; Vector Backbone:pTRIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_73704 Copy
Species: Mus musculus
Genetic Insert: RIPK3
Vector Backbone Description: Backbone Marker:GE Dharmacon; Backbone Size:12300; Vector Backbone:pTRIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_73703 Copy
Species: Mus musculus
Genetic Insert: RIPK3
Vector Backbone Description: Backbone Marker:GE Dharmacon; Backbone Size:12300; Vector Backbone:pTRIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_73702 Copy
Species: Mus musculus
Genetic Insert: RIPK3
Vector Backbone Description: Backbone Marker:GE Dharmacon; Backbone Size:12300; Vector Backbone:pTRIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_73705 Copy
Species: Homo sapiens
Genetic Insert: mCherry-TSC2
Vector Backbone Description: Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192440 Copy
Species: Homo sapiens
Genetic Insert: RAGE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
References:
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23645 Copy
Species: Other
Genetic Insert: Methyl coenzyme M reductase
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pMEV5; Vector Types:Synthetic Biology, Other, Heterologous expression in Methanococcus maripaludis; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192767 Copy
Species: Other
Genetic Insert: Methyl coenzyme M reductase
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pMEV5; Vector Types:Synthetic Biology, Other, Heterologous expression in Methanococcus maripaludis; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192769 Copy
Species:
Genetic Insert: SpCas9
Vector Backbone Description: Backbone Marker:Addgene Plasmid #62988; Vector Backbone:pX459; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192480 Copy
Species: Other
Genetic Insert: Methyl coenzyme M reductase
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pMEV5; Vector Types:Synthetic Biology, Other, Heterologous expression in Methanococcus maripaludis; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192775 Copy
Species: Other
Genetic Insert: Methyl coenzyme M reductase
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pMEV5; Vector Types:Synthetic Biology, Other, Heterologous expression in Methanococcus maripaludis; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192772 Copy
Species: Other
Genetic Insert: Methyl coenzyme M reductase
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pMEV5; Vector Types:Synthetic Biology, Other, Heterologous expression in Methanococcus maripaludis; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192777 Copy
Species: Homo sapiens
Genetic Insert: Barcoded piggyBac tdTomato SRT
Vector Backbone Description: Vector Backbone:AAV-PB-SRT-tdTomato; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_193187 Copy
Species: Other
Genetic Insert: Methyl coenzyme M reductase
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pMEV5; Vector Types:Synthetic Biology, Other, Heterologous expression in Methanococcus maripaludis; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192771 Copy
Species: Other
Genetic Insert: Methyl coenzyme M reductase
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pMEV5; Vector Types:Synthetic Biology, Other, Heterologous expression in Methanococcus maripaludis; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192770 Copy
Species: Homo sapiens
Genetic Insert: Barcoded piggyBac tdTomato SRT
Vector Backbone Description: Vector Backbone:AAV-PB-SRT-tdTomato; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_193185 Copy
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