Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector B-L
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 1 and 12 of EMMA platform.
Proper citation: RRID:Addgene_100645 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector A-C
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to fill in the gap between the backbone and parts placed in position 3 of EMMA platform.
Proper citation: RRID:Addgene_100640 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector N-R
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 13 and 18 of EMMA platform.
Proper citation: RRID:Addgene_100658 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector Q-R
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 16 and 18 of EMMA platform.
Proper citation: RRID:Addgene_100659 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector L-W
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 11 and 23 of EMMA platform.
Proper citation: RRID:Addgene_100655 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector L-Y
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 11 and 25 of EMMA platform.
Proper citation: RRID:Addgene_100656 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector L-M
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 11 and 13 of EMMA platform.
Proper citation: RRID:Addgene_100652 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector A-B
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to fill in the gap between the backbone and parts placed in position 2 of EMMA platform.
Proper citation: RRID:Addgene_100638 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector B-C
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 1 and 3 of EMMA platform.
Proper citation: RRID:Addgene_100639 Copy
Species: Synthetic
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Fluorescent reporter - Red - ?ex (nm) = 559 ?em (nm) = 600 . doi: 10.1038/nmeth.2171
Proper citation: RRID:Addgene_100687 Copy
Species: Synthetic
Genetic Insert: Insulator_FB
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: FII/BEAD-A (FB) element. (STEMCELLS2008;26:3257�3266 ). FII/BEAD-A (FB) contains the minimal enhancer-blocking components of the chicken b-globin 5'HS4 insulator and a homologous region from the human T-cell receptor BEAD-1 insulator
IMPORT NOTES: Special character found in field(Comments)
Proper citation: RRID:Addgene_100686 Copy
Species: Synthetic
Genetic Insert: DmrC
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DmrC is a chemically inducible dimerization domain.It dimerizes with DmrA in presence of heterodimerizer. iDimerizeTM Inducible Heterodimer System - Clontech
Proper citation: RRID:Addgene_100693 Copy
Species: Synthetic
Genetic Insert: Linker1
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Flexible peptide linker: SGLRSGSGGGSASGGSG . PROTEIN SCIENCE 2013 VOL 22:153�167
IMPORT NOTES: Special character found in field(Comments)
Proper citation: RRID:Addgene_100699 Copy
Species: Synthetic
Genetic Insert: Linker3
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Flexible peptide linker: GGGSGGGGSAS
Proper citation: RRID:Addgene_100695 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector for second step assembly
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function and internal BsaI site generating a ATCC overhang upon cleavage. It is used in the first step BsmBI-based Golden Gate along with HC_Kan_conn_BsaI-1_p1 to flank the vector with BsaI stes. Vectors built in the first BsmBI-based Golden Gate reaction will be flanked by BsaI sites that are used in a second step assembly using as entry vector HC_RFP-4 (the part-entry vector for position 4). See manuscript for futher information
Proper citation: RRID:Addgene_100669 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector Q-W
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 16 and 23 of EMMA platform.
Proper citation: RRID:Addgene_100660 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector W-Y
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 22 and 25 of EMMA platform.
Proper citation: RRID:Addgene_100665 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector W-X
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 22 and 24 of EMMA platform.
Proper citation: RRID:Addgene_100666 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector T-V
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 19 and 22 of EMMA platform.
Proper citation: RRID:Addgene_100662 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector U-V
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 20 and 22 of EMMA platform.
Proper citation: RRID:Addgene_100663 Copy
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