Searching the RRID Resource Information Network
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 Resource Report Resource Website 1+ mentions |
RRID:Addgene_124603 | Soma-localized ChrimsonR | Mus musculus | Ampicillin | PMID:30842660 | Backbone Marker:Genscript; Backbone Size:3000; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:22:02 | 1 | ||
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3XFlag-pA-Tn5-Fl Resource Report Resource Website 1+ mentions |
RRID:Addgene_124601 | Protein A and hyperactive Tn5 transposase (Tnp) fusion protein | Other | Ampicillin | PMID:31036827 | Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results to compare sequences. This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but can reduce transformation or transfection efficiencies. If you have trouble isolating the monomeric version of this plasmid, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid. | Backbone Size:8079; Vector Backbone:pTXB1-Tn5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:22:02 | 1 | |
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pMaCTag-Z07 Resource Report Resource Website 1+ mentions |
RRID:Addgene_124790 | mNeonGreen | Homo sapiens | Ampicillin | PMID:32406907 | Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint | Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:Other, PCR Template; Bacterial Resistance:Ampicillin | 2022-04-22 03:22:03 | 1 | |
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Flag-TRIM28 Resource Report Resource Website 1+ mentions |
RRID:Addgene_124960 | hTRIM28 | Homo sapiens | Ampicillin | PMID:28068325 | Backbone Marker:Barton Lab; Vector Backbone:pCMX-(Flag)x2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:22:05 | 1 | ||
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pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) Resource Report Resource Website 1+ mentions |
RRID:Addgene_121538 | HA-tagged hM4D(Gi) | Homo sapiens | Ampicillin | PMID:31063250 | Backbone Marker:NIDA_GEVVC; Backbone Size:4500; Vector Backbone:pOTTC1479 - pAAV SYN1 iRFP-FLAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:20:55 | 1 | ||
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pIS0 Resource Report Resource Website 1+ mentions |
RRID:Addgene_12178 | Ampicillin | PMID:15105502 | The firefly luciferase vector was modified from pGL3 Control Vector (Promega), such that a short sequence containing multiple cloning sites (5'-AGCTCTATACGCGTCTCAAGCTTACTGCTAGC GT-3') was inserted into the XbaI site immediately downstream from the stop codon. 3'UTR segments of target genes can be inserted into this vector to test for their effects on mRNA stability. | Backbone Marker:Bartel Lab; Backbone Size:5264; Vector Backbone:pIS0; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2022-04-22 03:20:58 | 3 | |||
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pCMV6M-PAK1 T423E Resource Report Resource Website 1+ mentions |
RRID:Addgene_12208 | Pak1 | Homo sapiens | Ampicillin | PMID:9395435 | There is also an additional mutation in this plasmid - L516I. The L516I mutation, or SNP, has been present from the very beginning (~1995). The crystal structure of Pak1 uses this clone, so it is present there too. The depositor does not think it affects function in any way. Pak1 may contain a G401S mutation. Depositor states that this mutation should not affect plasmid function. | Backbone Size:4900; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Activated Pak1: T423E | 2022-04-22 03:21:03 | 3 |
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pEBG-Pak1 83-149 Resource Report Resource Website 1+ mentions |
RRID:Addgene_12214 | Pak1 | Homo sapiens | Ampicillin | PMID:11719502 | Backbone Size:6100; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Dominant negative Pak1: autoinhibitory domain, aa 83-149 | 2022-04-22 03:21:04 | 1 | |
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pGEXTK-Pak1 70-117 Resource Report Resource Website 1+ mentions |
RRID:Addgene_12217 | Pak1 | Homo sapiens | Ampicillin | PMID: | Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | PBD (p21-binding domain), aa 70-117 | 2022-04-22 03:21:05 | 2 | |
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pCMV6M-Pak1 H83L H86L Resource Report Resource Website 1+ mentions |
RRID:Addgene_12211 | Pak1 | Homo sapiens | Ampicillin | PMID:9395435 | Created by 3-way ligation of PCR products: B/Sac-Sac/Eco-Eco/B. Pak1 may contain mutations G401S and L516I. Depositor states that this should not affect plasmid function. | Backbone Size:4900; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | PBD (p21-binding domain) mutant: H83L, H86L (see depositor comment below) | 2022-04-22 03:21:04 | 1 |
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pCMV6M-Pak1 L107F Resource Report Resource Website 1+ mentions |
RRID:Addgene_12212 | Pak1 | Homo sapiens | Ampicillin | PMID:11719502 | Addgene has found that this plasmid is extremely slow growing. We recommend keeping plates and liquid cultures growing for at least 20 hours. Overnight will not be sufficient. There is also an additional mutation in this plasmid - L516I. The L516I mutation, or SNP, has been present from the very beginning (~1995). The crystal structure of Pak1 uses this clone, so it is present there too. The depositor does not think it affects function in any way. Pak1 may contain a G401S mutation. Depositor states that this mutation should not affect plasmid function. | Backbone Size:4900; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Constitutively active Pak1: L107F | 2022-04-22 03:21:04 | 2 |
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pAAV-hsyn_NLS-His-rsCaMPARI-mRuby3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_122092 | rsCaMPARI | Synthetic | Ampicillin | PMID:32931424 | Backbone Size:4270; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:21:04 | 1 | ||
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pLPC-N MYC Resource Report Resource Website 1+ mentions |
RRID:Addgene_12540 | N MYC | Ampicillin | PMID: | Backbone Size:5600; Vector Backbone:pLPC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2022-04-22 03:22:09 | 1 | |||
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CMV-hRad51 Resource Report Resource Website 1+ mentions |
RRID:Addgene_125570 | Rad51 | Homo sapiens | Ampicillin | PMID:31101808 | Backbone Size:3300; Vector Backbone:CMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2022-04-22 03:22:11 | 2 | ||
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Cytosolic Syk Biosensor Resource Report Resource Website 1+ mentions |
RRID:Addgene_125729 | SYK biosensor | Synthetic | Ampicillin | PMID:25541586 | Backbone Marker:Invitrogen, modified at Dr. Roger Tsien Lab in University of California, San Diego; Backbone Size:5505; Vector Backbone:pcDNA3.1’; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:22:13 | 1 | ||
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pLOX-Ttag-iresTK Resource Report Resource Website 1+ mentions |
RRID:Addgene_12246 | SV40 Large T, HSV1-TK | Simian virus | Ampicillin | PMID:11020357 | CMV promoter is upstream of the bicistronic coding cassette with SV40-Large T and HSV1-TK. During reverse transcription U3 region of the 3'LTR, with LoxP, is duplicated; LoxP sites end up flanking the genome of the integrated provirus. Upon expression of Cre, provirus is excised. Please note that the full sequence for this plasmid is approximated and not fully verified--there will be discrepancies between Addgene's quality control sequence and the theoretical sequence from the depositor. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols. | Backbone Size:12290; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin | LoxP in 3' LTR. | 2022-04-22 03:21:09 | 1 |
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pLOX-TERT-iresTK Resource Report Resource Website 1+ mentions |
RRID:Addgene_12245 | TERT, HSV1-TK | Homo sapiens | Ampicillin | PMID:11020357 | CMV promoter is upstream of the bicistronic coding cassette with hTert and HSV1-TK. During reverse transcription U3 region of the 3'LTR, with LoxP, is duplicated; LoxP sites end up flanking the genome of the integrated provirus. Upon expression of Cre, provirus is excised. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. | Backbone Size:13115; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin | LoxP in 3' LTR. | 2022-04-22 03:21:09 | 3 |
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pCrl Resource Report Resource Website 1+ mentions |
RRID:Addgene_122443 | reelin | Mus musculus | Ampicillin | PMID:8987733 | The entire reelin open reading frame was assembled by fusing five overlapping cDNA clones isolated previously (D’Arcangelo et al., 1995) and subcloning them into pcDNA3. The following reelin fragments were used: 1.2 kb NaeI–SalI from p59BS1, 1.3 kb SalI–NdeI from pBS2, 3.1 kb NdeI–BspEI from pBS6, 770 bp BspEI–ApaLI from p3Rea3, and 4.2 kb ApaLIEcoRV from pBS53. The final clone (pCrl) contains the entire reelin open reading frame (10,383 bp) plus 95 bp of sequence 59 to the initiator methionine codon and 82 bp of 39 untranslated sequence (reelin cDNA nucleotides 188–10,748). | Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:21:09 | 1 | |
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pAAV-Syn-iCre Resource Report Resource Website 1+ mentions |
RRID:Addgene_122518 | iCre | Ampicillin | PMID: | Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:21:10 | 4 | |||
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pRSV-Rev Resource Report Resource Website 50+ mentions |
RRID:Addgene_12253 | Rev | Ampicillin | PMID:9765382 | Rev cDNA expressing plasmid in which the joined second and third exons of HIV-1 rev are under the transcriptional control of RSV U3 promoter. Part of the 3rd generation packaging system. Please note that most of Trono lab lentiviral vectors can only be used with the 2nd generation packaging system. See notes for pMDLg/pRRE. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussions on cloning strategies and protocols. | Backbone Size:4174; Vector Backbone:pRSV-Rev; Vector Types:Mammalian Expression, Lentiviral, Other, Packaging; Bacterial Resistance:Ampicillin | 2022-04-22 03:21:11 | 68 |
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