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http://www.addgene.org/45937

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:SEVA (de Lorenzo Lab); Vector Backbone:pSEVA424; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: This plasmid was tested in the Gram-negative soil bacterium Pseudomonas putida KT2440 and Escherichia coli K12 and is especially suited for protein production, affinity purification, protein complex copurification with SPINE (Strep Protein Interaction Experiments) or (co-)localization studies. Due to the broad host range of the RK2 origin of replication, the plasmid facilitates experimental verification of hypothetical proteins and protein production yield assessment in different expression hosts possibly including new isolates. The Supplementary Table S1 in the following publication lists approximately 30 strains in which the RK2 origin of replication should be functional. Silva-Rocha et al., The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes. Nucleic Acids Research 2013, 41:D666-675. http://nar.oxfordjournals.org/content/41/D1/D666.long

Proper citation: RRID:Addgene_45937 Copy   

•   Source: Addgene

http://www.addgene.org/45939

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:SEVA (de Lorenzo Lab); Vector Backbone:pSEVA424; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: This plasmid was tested in the Gram-negative soil bacterium Pseudomonas putida KT2440 and Escherichia coli K12 and is especially suited for protein production, affinity purification, protein complex copurification with SPINE (Strep Protein Interaction Experiments) or (co-)localization studies. Due to the broad host range of the RK2 origin of replication, the plasmid facilitates experimental verification of hypothetical proteins and protein production yield assessment in different expression hosts possibly including new isolates. The Supplementary Table S1 in the following publication lists approximately 30 strains in which the RK2 origin of replication should be functional. Silva-Rocha et al., The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes. Nucleic Acids Research 2013, 41:D666-675. http://nar.oxfordjournals.org/content/41/D1/D666.long

Proper citation: RRID:Addgene_45939 Copy   

•   Source: Addgene

http://www.addgene.org/175241

Species: Homo sapiens
Genetic Insert: SAE1
Vector Backbone Description: Backbone Marker:Novagen - lab stock; Backbone Size:3600; Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_175241 Copy   

•   Source: Addgene

http://www.addgene.org/175579

Species: Other
Genetic Insert: PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, CRISPR(BsaI)
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_175579 Copy   

•   Source: Addgene

http://www.addgene.org/24545

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Streptomycin
References:
Comments: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZ M15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu) 7649 galU galK rspL nupG [ λcI857 (cro-bioA) < > araC-PBADtrfA]

Proper citation: RRID:Addgene_24545 Copy   

•   Source: Addgene

http://www.addgene.org/31288

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4553; Vector Backbone:pTC-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_31288 Copy   

•   Source: Addgene

http://www.addgene.org/169765

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pHAtC; Vector Types:Plant Expression, Other, Plant Binary vector; Bacterial Resistance:Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_169765 Copy   

•   Source: Addgene

http://www.addgene.org/170279

Species: Other
Genetic Insert: HIV-2 RT p66 (mutant)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3498; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: Please visit https://www.medrxiv.org/content/10.1101/2020.08.13.20173757v4 for medRxiv preprint

Proper citation: RRID:Addgene_170279 Copy   

•   Source: Addgene

http://www.addgene.org/170277

Species: Other
Genetic Insert: Bst-LF D720A
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3543; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: Please visit https://www.medrxiv.org/content/10.1101/2020.08.13.20173757v4 for medRxiv preprint

Proper citation: RRID:Addgene_170277 Copy   

•   Source: Addgene

http://www.addgene.org/172557

Species: Synthetic
Genetic Insert: LuxR-mRFP
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:original Duet vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_172557 Copy   

•   Source: Addgene

http://www.addgene.org/175043

Species: Homo sapiens
Genetic Insert: Replication factor C subunit 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_175043 Copy   

•   Source: Addgene

http://www.addgene.org/49796

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5000; Vector Backbone:pCDFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_49796 Copy   

•   Source: Addgene

http://www.addgene.org/49763

Species:
Genetic Insert: genotype: rpsL, thr, leu, thi, lacY, galK, galT, ara, tomA, tsx, dam, dcm, supE44, Δ(lac-proAB), [F' traD36, proAB, lacIqZΔM15]
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_49763 Copy   

•   Source: Addgene

http://www.addgene.org/70255

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:6798; Vector Backbone:see comments; Vector Types:Other, Conditionally lethal; Bacterial Resistance:Streptomycin
References:
Comments: sacB was derived from pUM24 (Ried, J. L., and A. Colimer Gene 57:239-246, 1987) and from pUCD800 (Gay, et al. 1985 J. Bacteriol. 164:918-921). The streptomycin/spectinomycin determinant in pRL277 was derived from the omega cassette (Prentki, et al. Gene 103: 17-23 1991). Additional Reference for this plasmid: Black TA, Cai Y, Wolk CP (1993) Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Mol Microbiol 9: 77-84. Publisher’s correction (1993): Mol Microbiol 10: 1153

Proper citation: RRID:Addgene_70255 Copy   

•   Source: Addgene

http://www.addgene.org/72516

Species: Other
Genetic Insert: formylglycinamidine ribonucleotide synthetase, small subunit
Vector Backbone Description: Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: pCloDF13 replicon

Proper citation: RRID:Addgene_72516 Copy   

•   Source: Addgene

http://www.addgene.org/72517

Species: Other
Genetic Insert: formylglycinamidine ribonucleotide synthetase, small subunit
Vector Backbone Description: Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: pCloDF13 replicon

Proper citation: RRID:Addgene_72517 Copy   

•   Source: Addgene

http://www.addgene.org/72520

Species: Other
Genetic Insert: formylglycinamidine ribonucleotide synthetase, large subunit
Vector Backbone Description: Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: pCloDF13 replicon

Proper citation: RRID:Addgene_72520 Copy   

•   Source: Addgene

http://www.addgene.org/72450

Species: Other
Genetic Insert: N5-carboxyaminoimidazole ribonucleotide mutase
Vector Backbone Description: Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: CDF ori

Proper citation: RRID:Addgene_72450 Copy   

•   Source: Addgene

http://www.addgene.org/72449

Species: Other
Genetic Insert: N5-carboxyaminoimidazole ribonucleotide synthetase
Vector Backbone Description: Backbone Size:3781; Vector Backbone:pCDFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: CDF ori

Proper citation: RRID:Addgene_72449 Copy   

•   Source: Addgene

http://www.addgene.org/72676

Species: Other
Genetic Insert: Cas1, Cas2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_72676 Copy   

•   Source: Addgene