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  • Bacterial Resistance:spectinomycin and streptomycin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
28 pCOLI_S_STREP
 
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RRID:Addgene_160262 Spectinomycin and Streptomycin PMID:32955754 Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin 2022-04-22 03:30:00 0
29 pCOLI_S_C_STREP
 
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RRID:Addgene_160171 Spectinomycin and Streptomycin PMID:32955754 Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin 2022-04-22 03:29:59 0
27 pCOLI_S_C_HIS
 
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RRID:Addgene_160169 Spectinomycin and Streptomycin PMID:32955754 Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin 2022-04-22 03:29:59 0
pWY82
 
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RRID:Addgene_65721 Telomere Arabidopsis thaliana Spectinomycin and Streptomycin PMID:17085598 Addgene was unable to sequence this plasmid due to the repetitive nature of the telomere repeats. The plasmid was instead verified by restriction digest. The gel image is provided on this page under- Resource Information- Addgene Notes. Backbone Size:9189; Vector Backbone:pTF101.1; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin 2022-04-22 03:46:34 0
pRL692
 
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RRID:Addgene_70284 Transposon Tn5-692 Other Spectinomycin and Streptomycin PMID:12218043 Tn5 derivative Tn5-692 (in plasmid pRL692; GenBank accession no. AF424805) contains a pMB1 oriV; and bears mutations (Zhou, et al. 1998 J. Mol. Biol. 276:913–925) that increase its rate of transposition ca. 100-fold relative to that of Tn5-1058 (Wolk, et al. 1991. Proc. Natl. Acad. Sci. USA 88:5355–5359), providing large numbers of transposon mutants. The erythromycin-resistance gene came from Horinouchi and Weisblum (J. Bacteriol. 150: 804-814, 1982) and the streptomycin-spectinomycin gene came from the Ω-fragment described by Prentki and Krisch (Gene 29, 303-313, 1984) and was purchased as an EcoRI fragment from Amersham. NOTE--Because Tn5-692 appears able to transpose repeatedly, the user is cautioned not to delay storing strains whose phenotype is of interest. Additional references: Miyagishima S-y, Wolk CP, Osteryoung KW (2005) Identification of cyanobacterial cell division genes by comparative and mutational analyses. Mol Microbiol 56: 126-143 Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin 2022-04-22 03:47:45 0
pRL1383a
 
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RRID:Addgene_70692 Spectinomycin and Streptomycin PMID:17639350 MCS: (PT7→)HindIII-SphI-PstI(SbfI)-SalI(HincII)-KpnI-SacI-EcoRI-ClaI-ApaI-SacII-NsiI-NdeI-BglII-AflII-StuI-AvrII-XbaI-AatII-BsiWI-XhoI(PspXI)-SrfI-AscI-RsrII-BamHI(←PSP6) Vector Backbone:RSF1010; Vector Types:Other, Bacterial shuttle vector; Bacterial Resistance:Spectinomycin and Streptomycin 2022-04-22 03:47:51 0
pTargetF-FRT
 
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RRID:Addgene_113637 sgRNA-FRT Synthetic Spectinomycin and Streptomycin PMID:29884781 Vector Backbone:pTargetF; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin and Streptomycin 2022-04-22 03:18:21 0
pEA106
 
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RRID:Addgene_187872 mCherry Other Spectinomycin and Streptomycin PMID:35690588 Backbone Marker:Keunsub Lee; Backbone Size:6149; Vector Backbone:pTF505; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin 2022-08-07 01:02:32 0
KFP13
 
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RRID:Addgene_119795 Synpcc7942_1849 Spectinomycin and Streptomycin PMID:30540929 Vector Backbone:Vector for targeting integration at neutral site 1 in ATCC PCC7942 genome; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin 2022-04-22 03:20:23 0
KFP14
 
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RRID:Addgene_119796 Synpcc7942_1557 Spectinomycin and Streptomycin PMID:30540929 Vector Backbone:Vector for targeting integration at neutral site 1 in ATCC PCC7942 genome; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin 2022-04-22 03:20:23 0
pAM5188
 
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RRID:Addgene_120087 pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS). Other Spectinomycin and Streptomycin PMID:27902432 Backbone Size:6936; Vector Backbone:pAM4788; Vector Types:Other, other; Bacterial Resistance:Spectinomycin and Streptomycin 2022-04-22 03:20:32 0
pTrc99S-ssDsbA-TTlight-4xDQNAT
 
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RRID:Addgene_128402 Tetanus toxin light chain domain Other Spectinomycin and Streptomycin PMID:33536221 Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin Inactivating E234A mutation 2022-04-22 03:23:18 0
pTrc99S-ssDsbA-EPA-DNNNS-DQNRT
 
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RRID:Addgene_128404 Exotoxin A Other Spectinomycin and Streptomycin PMID:33536221 Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin 242DNNNS246 and 384DQNRT388 engineered glycosylation sites 2022-04-22 03:23:18 0
pTrc99S-ssDsbA-PD-4xDQNAT
 
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RRID:Addgene_128399 H. influenzae protein D Other Spectinomycin and Streptomycin PMID:33536221 Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin 2022-04-22 03:23:18 0
pCrepusculo
 
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RRID:Addgene_190156 Spectinomycin and Streptomycin PMID:36129831 Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin 2022-12-17 12:04:13 0
P0050::kaiBC S431E T432E NS1
 
Resource Report
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RRID:Addgene_101836 kaiBC S431E T432E with p0050 promoter Other Spectinomycin and Streptomycin PMID:28430105 Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Other, Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin S431E T432E 2022-04-22 03:15:11 0
Ptrc::zwf_fbp_opcA
 
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RRID:Addgene_101846 sp/st resistance casette followed by ptrc promoter Other Spectinomycin and Streptomycin PMID:28430105 Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Other, Cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin 2022-04-22 03:15:11 0
rpaA_NS1
 
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RRID:Addgene_101829 RpaA with its native promoter Other Spectinomycin and Streptomycin PMID:28430105 Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Other, Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin 2022-04-22 03:15:10 0
relAE335Q_NS1
 
Resource Report
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RRID:Addgene_100917 RelA fragment from E.coli with point mutation E335Q Other Spectinomycin and Streptomycin PMID:29241543 Backbone Size:5700; Vector Backbone:pam1303; Vector Types:Other, Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin E 335 converted to Q 2022-04-22 03:14:58 0
pAM2991
 
Resource Report
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RRID:Addgene_40248 One Step Cloneing Vector for Overexpression Synechococcus elongatus PCC 7942 Spectinomycin and Streptomycin PMID:15775978 pAM2990 was partially digested with SacI and re-ligatedto remove the antihel fragment. The net result is the insertion of the BglII fragment from pAM2255 into BamHI digested pAM1303 in the orientation that Ptrc goes away from the SacI site in pAM1303 (SacI digestion gives a 1.7 KB band). This plasmid can be used as a one-step cloning vector for overexpression. Vector Backbone:pAM2990; Vector Types:Other, Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin 2023-04-01 01:06:15 0

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