Plasmids   Select Another Resource Report Type

http://www.addgene.org/202242

Species: Synthetic
Genetic Insert: bc110
Vector Backbone Description: Vector Backbone:pBCC090; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.

Proper citation: RRID:Addgene_202242 Copy   

•   Source: Addgene

http://www.addgene.org/202256

Species: Synthetic
Genetic Insert: bc156
Vector Backbone Description: Vector Backbone:pBCC099; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.

Proper citation: RRID:Addgene_202256 Copy   

•   Source: Addgene

http://www.addgene.org/202255

Species: Synthetic
Genetic Insert: bc155
Vector Backbone Description: Vector Backbone:pBCC099; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.

Proper citation: RRID:Addgene_202255 Copy   

•   Source: Addgene

http://www.addgene.org/202261

Species: Synthetic
Genetic Insert: bc161
Vector Backbone Description: Vector Backbone:pBCC101; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.

Proper citation: RRID:Addgene_202261 Copy   

•   Source: Addgene

http://www.addgene.org/202262

Species: Synthetic
Genetic Insert: bc162
Vector Backbone Description: Vector Backbone:pBCC101; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.

Proper citation: RRID:Addgene_202262 Copy   

•   Source: Addgene

http://www.addgene.org/179486

Species: Other
Genetic Insert: none
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Other
References:
Comments: λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5] F- araC14 fhuA31 lacY1 tsx-78 ΔompF480 zcb-222::Tn10 rfbC1 mgl-51 ΔompC178 rpsL136(StrR) xylA5 mtl-1 thiE1 KJ740(DE3) is a λDE3 derivative of the OmpC- & OmpF-deficient strain KJ740 that was described in Ingham C, Buechner M, Adler J, 1990, J Bacteriol 172:3577-3583. Due to the presence of the lambda (DE3) lysogen, KJ740(DE3) is compatible with expression from the T7 promoter. The (DE3) lysogen of the parent strain KJ740 was made by Debra T. Hansen using the λDE3 Lysogenization Kit 538 (EMD Millipore #69734-3). The parent strain KJ740 was obtained from the Yale E. coli Genetic Stock Center (CGSC) as strain number 12151. In contrast to BL21 derivative strains, KJ740(DE3) is not deficient in the proteases Lon and OmpT. The presence of (DE3) was confirmed by PCR by Felicia M. Craciunescu. PCR primers that may be used to verify the presence of the (DE3) lysogen: These primer pairs are expected to generate a PCR product from each of 3 genes within the (DE3) sequence, GenBank accession no. AM946981.2. There are two primer pairs per gene, therefore 6 possible PCR products. The presence of the PCR product of the expected size indicates the presence of the (DE3) lysogen. The PCR reactions will also be run on the negative control non-(DE3) parent strain, KJ740, which is expected to not generate these PCR product sizes. gene A F1 GACTACATCCGTGAGGTGAATGTGG gene A R1 GATGACGCAGGCATTATGCTCGCAG Product size = 645 bp gene A F2 CCTTTCACATCTGGACAGCGTACAG gene A R2 GTTGCTGCACCATCCTCTTCCTGC Product size = 904 bp gene H F1 GACATCTGGAATCTGCGCAAGGATGA gene H R1 CTGCAGGATTTTCCCGTCTTTCAGTG Product size = 291 bp gene H F2 GTGCGCTGGCGTATGCCTGGTATC gene H R2 CTGTTCCGTGGCTTCCCGTTCTGC Product size = 1388 bp gene J F1 CTTTACCTTCGGTGTACAGGCACTGGTG gene J R1 GTCCTGCACGAACGTCAGCGTCTG Product size = 748 bp gene J F2 CAGACAGGTTGAAACCAGCACGCGTTATC gene J R2 GTTTGCCTTCCTCCGTGTCCTCCATG Product size = 407 bp Please visit https://www.biorxiv.org/content/10.1101/2021.08.26.457821v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_179486 Copy   

•   Source: Addgene

http://www.addgene.org/218768

Species: Other
Genetic Insert: EcNR1 pUltraG ScW40 CCA trpS::ZeoR trpT::gentR dgalK lambdaRED::galK
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_218768 Copy   

•   Source: Addgene

http://www.addgene.org/218769

Species: Other
Genetic Insert: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_218769 Copy   

•   Source: Addgene

http://www.addgene.org/229958

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Other
References:
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.

Proper citation: RRID:Addgene_229958 Copy   

•   Source: Addgene

http://www.addgene.org/229959

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Other
References:
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.

Proper citation: RRID:Addgene_229959 Copy   

•   Source: Addgene

http://www.addgene.org/22816

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5922; Vector Backbone:pBMTL-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_22816 Copy   

•   Source: Addgene

http://www.addgene.org/22810

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4677; Vector Backbone:pBTL-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: Addgene's QC sequence shows a 1bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity.

Proper citation: RRID:Addgene_22810 Copy   

•   Source: Addgene

http://www.addgene.org/22829

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4617; Vector Backbone:pBT-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: Addgene's QC sequence shows a 1bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity.

Proper citation: RRID:Addgene_22829 Copy   

•   Source: Addgene

http://www.addgene.org/22822

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5799; Vector Backbone:pBTB-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_22822 Copy   

•   Source: Addgene

http://www.addgene.org/156372

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Other
References:
Comments: For this strain the depositor have verified the appropriate antibiotic resistances, validated sequencing of relevant regions, and performed a restriction enzyme digest to confirm the expected cut sites Depositor recomments the following primers to sequence the relevant regions: gaaattccttgtcgggtaagttcc gaacatcaaacattaaagggtggtatttc Depositor also recommends testing for antibiotic resistance alongside the sequencing and digestion verification. Protocol for the digestion reaction: For 50 uL reaction: 1 ug DNA 5 uL CutSmart 1 uL HpyCH4III Up to 50 uL MQ water Depositor confirmed that the resulting bands were confirmed to be shorter than the starting template

Proper citation: RRID:Addgene_156372 Copy   

•   Source: Addgene

http://www.addgene.org/42520

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript KS-; Vector Types:Bacterial Expression, Other, Unspecified, Cloning; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_42520 Copy   

•   Source: Addgene

http://www.addgene.org/42521

Species:
Genetic Insert: EF1a-DTA
Vector Backbone Description: Vector Backbone:pF2; Vector Types:Mouse Targeting; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_42521 Copy   

•   Source: Addgene

http://www.addgene.org/42519

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript KS-; Vector Types:Bacterial Expression, Other, Unspecified, Cloning; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_42519 Copy   

•   Source: Addgene

http://www.addgene.org/63726

Species: Homo sapiens
Genetic Insert: Mini-intronic-plasmid intron
Vector Backbone Description: Backbone Marker:Joseph Wu's Lab at Stanford University; Backbone Size:8062; Vector Backbone:codon-optimized minicircle (CoMiC) construct; Vector Types:Mammalian Expression; Bacterial Resistance:Other
References:
Comments: In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514.

Proper citation: RRID:Addgene_63726 Copy   

•   Source: Addgene

http://www.addgene.org/86400

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:strain: SJ_XTL219; Vector Types:CRISPR; Bacterial Resistance:Other
References:
Comments: THIS IS A STRAIN. SJ_XTL219 is an E.coli K12 derivative of MG1655. It contains a tunable arabinose operon promoter PBAD to quantitatively control the expression of CRISPR-dCas9 protein over two orders of magnitude in a plasmid-free system. This tCRISPRi is reversible, and gene expression can be repressed or restored by adding or withdrawing arabinose. This tCRISPRi strain has less than 10% leaky expression. Most importantly from a practical perspective, construction of tCRISPRi to target a new gene requires only one-step of oligo recombineering to add the sgRNA. This tCRISPRi strain, in combination with recombineering, provides a simple and easy-to-implement tool for gene expression control, and is ideally suited for construction of both individual strains and high-throughput tunable knockdown libraries.

Proper citation: RRID:Addgene_86400 Copy   

•   Source: Addgene