| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
pBC082 Resource Report Resource Website |
RRID:Addgene_202241 | bc109 | Synthetic | Other | PMID:38503974 | This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint. | Vector Backbone:pBCC090; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2024-04-10 01:05:32 | 0 | |
|
pBC083 Resource Report Resource Website |
RRID:Addgene_202242 | bc110 | Synthetic | Other | PMID:38503974 | This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint. | Vector Backbone:pBCC090; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2024-04-10 01:05:32 | 0 | |
|
pBC129 Resource Report Resource Website |
RRID:Addgene_202256 | bc156 | Synthetic | Other | PMID:38503974 | This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint. | Vector Backbone:pBCC099; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2024-04-10 01:05:33 | 0 | |
|
pBC128 Resource Report Resource Website |
RRID:Addgene_202255 | bc155 | Synthetic | Other | PMID:38503974 | This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint. | Vector Backbone:pBCC099; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2024-04-10 01:05:33 | 0 | |
|
pBC134 Resource Report Resource Website |
RRID:Addgene_202261 | bc161 | Synthetic | Other | PMID:38503974 | This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint. | Vector Backbone:pBCC101; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2024-04-10 01:05:33 | 0 | |
|
pBC135 Resource Report Resource Website |
RRID:Addgene_202262 | bc162 | Synthetic | Other | PMID:38503974 | This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint. | Vector Backbone:pBCC101; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2024-04-10 01:05:33 | 0 | |
|
ATMW1 Resource Report Resource Website |
RRID:Addgene_218768 | EcNR1 pUltraG ScW40 CCA trpS::ZeoR trpT::gentR dgalK lambdaRED::galK | Other | Other | PMID:28192410 | Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2024-08-01 01:07:04 | 0 | ||
|
ATMW-BL21 Resource Report Resource Website |
RRID:Addgene_218769 | pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR | Other | Other | PMID:34655653 | Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2024-08-01 01:07:04 | 0 | ||
|
psBIG1b Resource Report Resource Website |
RRID:Addgene_229958 | Other | PMID: | Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint. | Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Other | 2025-02-21 01:09:00 | 0 | |||
|
psBIG1c Resource Report Resource Website |
RRID:Addgene_229959 | Other | PMID: | Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint. | Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Other | 2025-02-21 01:09:00 | 0 | |||
|
pBMTL-6 Resource Report Resource Website |
RRID:Addgene_22816 | Other | PMID:16496398 | Backbone Size:5922; Vector Backbone:pBMTL-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2022-04-22 03:36:08 | 0 | ||||
|
pBTL-6 Resource Report Resource Website |
RRID:Addgene_22810 | Other | PMID:16496398 | Addgene's QC sequence shows a 1bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity. | Backbone Size:4677; Vector Backbone:pBTL-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2022-04-22 03:36:08 | 0 | |||
|
pBT-6 Resource Report Resource Website |
RRID:Addgene_22829 | Other | PMID:16496398 | Addgene's QC sequence shows a 1bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity. | Backbone Size:4617; Vector Backbone:pBT-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2022-04-22 03:36:08 | 0 | |||
|
pBTB-6 Resource Report Resource Website |
RRID:Addgene_22822 | Other | PMID:16496398 | Backbone Size:5799; Vector Backbone:pBTB-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2022-04-22 03:36:08 | 0 | ||||
|
S4246 Resource Report Resource Website |
RRID:Addgene_156372 | Other | PMID:33500394 | For this strain the depositor have verified the appropriate antibiotic resistances, validated sequencing of relevant regions, and performed a restriction enzyme digest to confirm the expected cut sites Depositor recomments the following primers to sequence the relevant regions: gaaattccttgtcgggtaagttcc gaacatcaaacattaaagggtggtatttc Depositor also recommends testing for antibiotic resistance alongside the sequencing and digestion verification. Protocol for the digestion reaction: For 50 uL reaction: 1 ug DNA 5 uL CutSmart 1 uL HpyCH4III Up to 50 uL MQ water Depositor confirmed that the resulting bands were confirmed to be shorter than the starting template | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Other | 2022-04-22 03:29:09 | 0 | |||
|
pF2 Resource Report Resource Website |
RRID:Addgene_42520 | Other | PMID:23437314 | Backbone Marker:Stratagene; Vector Backbone:pBluescript KS-; Vector Types:Bacterial Expression, Other, Unspecified, Cloning; Bacterial Resistance:Other | 2022-04-22 03:40:45 | 0 | ||||
|
pF2-DTA Resource Report Resource Website |
RRID:Addgene_42521 | EF1a-DTA | Other | PMID:23437314 | Vector Backbone:pF2; Vector Types:Mouse Targeting; Bacterial Resistance:Other | 2022-04-22 03:40:45 | 0 | |||
|
pF Resource Report Resource Website |
RRID:Addgene_42519 | Other | PMID:23437314 | Backbone Marker:Stratagene; Vector Backbone:pBluescript KS-; Vector Types:Bacterial Expression, Other, Unspecified, Cloning; Bacterial Resistance:Other | 2022-04-22 03:40:45 | 0 | ||||
|
MIP 247 CoMiP 4in1 with shRNA p53 Resource Report Resource Website 1+ mentions |
RRID:Addgene_63726 | Mini-intronic-plasmid intron | Homo sapiens | Other | PMID:25628230 | In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514. | Backbone Marker:Joseph Wu's Lab at Stanford University; Backbone Size:8062; Vector Backbone:codon-optimized minicircle (CoMiC) construct; Vector Types:Mammalian Expression; Bacterial Resistance:Other | codon-optimised | 2022-04-22 03:46:13 | 1 |
|
Jun lab tunable CRISPRi strain Resource Report Resource Website |
RRID:Addgene_86400 | Other | PMID:27996021 | THIS IS A STRAIN. SJ_XTL219 is an E.coli K12 derivative of MG1655. It contains a tunable arabinose operon promoter PBAD to quantitatively control the expression of CRISPR-dCas9 protein over two orders of magnitude in a plasmid-free system. This tCRISPRi is reversible, and gene expression can be repressed or restored by adding or withdrawing arabinose. This tCRISPRi strain has less than 10% leaky expression. Most importantly from a practical perspective, construction of tCRISPRi to target a new gene requires only one-step of oligo recombineering to add the sgRNA. This tCRISPRi strain, in combination with recombineering, provides a simple and easy-to-implement tool for gene expression control, and is ideally suited for construction of both individual strains and high-throughput tunable knockdown libraries. | Vector Backbone:strain: SJ_XTL219; Vector Types:CRISPR; Bacterial Resistance:Other | 2022-04-22 03:52:08 | 0 |
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