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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pBC082
 
Resource Report
Resource Website
RRID:Addgene_202241 bc109 Synthetic Other PMID:38503974 This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint. Vector Backbone:pBCC090; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2024-04-10 01:05:32 0
pBC083
 
Resource Report
Resource Website
RRID:Addgene_202242 bc110 Synthetic Other PMID:38503974 This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint. Vector Backbone:pBCC090; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2024-04-10 01:05:32 0
pBC129
 
Resource Report
Resource Website
RRID:Addgene_202256 bc156 Synthetic Other PMID:38503974 This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint. Vector Backbone:pBCC099; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2024-04-10 01:05:33 0
pBC128
 
Resource Report
Resource Website
RRID:Addgene_202255 bc155 Synthetic Other PMID:38503974 This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint. Vector Backbone:pBCC099; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2024-04-10 01:05:33 0
pBC134
 
Resource Report
Resource Website
RRID:Addgene_202261 bc161 Synthetic Other PMID:38503974 This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint. Vector Backbone:pBCC101; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2024-04-10 01:05:33 0
pBC135
 
Resource Report
Resource Website
RRID:Addgene_202262 bc162 Synthetic Other PMID:38503974 This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint. Vector Backbone:pBCC101; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2024-04-10 01:05:33 0
ATMW1
 
Resource Report
Resource Website
RRID:Addgene_218768 EcNR1 pUltraG ScW40 CCA trpS::ZeoR trpT::gentR dgalK lambdaRED::galK Other Other PMID:28192410 Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2024-08-01 01:07:04 0
ATMW-BL21
 
Resource Report
Resource Website
RRID:Addgene_218769 pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR Other Other PMID:34655653 Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2024-08-01 01:07:04 0
psBIG1b
 
Resource Report
Resource Website
RRID:Addgene_229958 Other PMID: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint. Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Other 2025-02-21 01:09:00 0
psBIG1c
 
Resource Report
Resource Website
RRID:Addgene_229959 Other PMID: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint. Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Other 2025-02-21 01:09:00 0
pBMTL-6
 
Resource Report
Resource Website
RRID:Addgene_22816 Other PMID:16496398 Backbone Size:5922; Vector Backbone:pBMTL-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2022-04-22 03:36:08 0
pBTL-6
 
Resource Report
Resource Website
RRID:Addgene_22810 Other PMID:16496398 Addgene's QC sequence shows a 1bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity. Backbone Size:4677; Vector Backbone:pBTL-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2022-04-22 03:36:08 0
pBT-6
 
Resource Report
Resource Website
RRID:Addgene_22829 Other PMID:16496398 Addgene's QC sequence shows a 1bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity. Backbone Size:4617; Vector Backbone:pBT-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2022-04-22 03:36:08 0
pBTB-6
 
Resource Report
Resource Website
RRID:Addgene_22822 Other PMID:16496398 Backbone Size:5799; Vector Backbone:pBTB-6; Vector Types:Bacterial Expression; Bacterial Resistance:Other 2022-04-22 03:36:08 0
S4246
 
Resource Report
Resource Website
RRID:Addgene_156372 Other PMID:33500394 For this strain the depositor have verified the appropriate antibiotic resistances, validated sequencing of relevant regions, and performed a restriction enzyme digest to confirm the expected cut sites Depositor recomments the following primers to sequence the relevant regions: gaaattccttgtcgggtaagttcc gaacatcaaacattaaagggtggtatttc Depositor also recommends testing for antibiotic resistance alongside the sequencing and digestion verification. Protocol for the digestion reaction: For 50 uL reaction: 1 ug DNA 5 uL CutSmart 1 uL HpyCH4III Up to 50 uL MQ water Depositor confirmed that the resulting bands were confirmed to be shorter than the starting template Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Other 2022-04-22 03:29:09 0
pF2
 
Resource Report
Resource Website
RRID:Addgene_42520 Other PMID:23437314 Backbone Marker:Stratagene; Vector Backbone:pBluescript KS-; Vector Types:Bacterial Expression, Other, Unspecified, Cloning; Bacterial Resistance:Other 2022-04-22 03:40:45 0
pF2-DTA
 
Resource Report
Resource Website
RRID:Addgene_42521 EF1a-DTA Other PMID:23437314 Vector Backbone:pF2; Vector Types:Mouse Targeting; Bacterial Resistance:Other 2022-04-22 03:40:45 0
pF
 
Resource Report
Resource Website
RRID:Addgene_42519 Other PMID:23437314 Backbone Marker:Stratagene; Vector Backbone:pBluescript KS-; Vector Types:Bacterial Expression, Other, Unspecified, Cloning; Bacterial Resistance:Other 2022-04-22 03:40:45 0
MIP 247 CoMiP 4in1 with shRNA p53
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_63726 Mini-intronic-plasmid intron Homo sapiens Other PMID:25628230 In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514. Backbone Marker:Joseph Wu's Lab at Stanford University; Backbone Size:8062; Vector Backbone:codon-optimized minicircle (CoMiC) construct; Vector Types:Mammalian Expression; Bacterial Resistance:Other codon-optimised 2022-04-22 03:46:13 1
Jun lab tunable CRISPRi strain
 
Resource Report
Resource Website
RRID:Addgene_86400 Other PMID:27996021 THIS IS A STRAIN. SJ_XTL219 is an E.coli K12 derivative of MG1655. It contains a tunable arabinose operon promoter PBAD to quantitatively control the expression of CRISPR-dCas9 protein over two orders of magnitude in a plasmid-free system. This tCRISPRi is reversible, and gene expression can be repressed or restored by adding or withdrawing arabinose. This tCRISPRi strain has less than 10% leaky expression. Most importantly from a practical perspective, construction of tCRISPRi to target a new gene requires only one-step of oligo recombineering to add the sgRNA. This tCRISPRi strain, in combination with recombineering, provides a simple and easy-to-implement tool for gene expression control, and is ideally suited for construction of both individual strains and high-throughput tunable knockdown libraries. Vector Backbone:strain: SJ_XTL219; Vector Types:CRISPR; Bacterial Resistance:Other 2022-04-22 03:52:08 0

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