Plasmids   Select Another Resource Report Type

http://www.addgene.org/135657

Species: Synthetic
Genetic Insert: Intron containing lox site
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pAT19; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Erythromycin
References:
Comments: Use in combination with pQlox71F (addgene 135655) or pQlox71R (addgene 135656), then pQcre1 (addgene 135659). Transcription of LtrB-derived group II intron is driven by the pFer promoter. Redesign intron to target gene using TargeTron or Clostron algorithms (links below), then clone by SOE-PCR or gene synthesis ligation between NdeI and BsrGI sites. Lox66 and Lox71 must be in same orientation to delete the intervening sequence, but the directions of introns relative to one another can generate inverted repeats in the chromosome after deletion has taken place, leading to futher recombinations events. See publication for details. Intron redesign links http://www.clostron.com/clostron1.php http://www.targetrons.com/targetron_pLtrB.php Reference for intron redesign Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP. The ClosTron: A universal gene knock-out system for the genus Clostridium. J Microbiol Methods. 2007;70(3):452-464. Perutka J, Wang W, Goerlitz D, Lambowitz AM. Use of Computer-designed Group II Introns to Disrupt Escherichia coli DExH / D-box Protein and DNA Helicase Genes. 2004:421-439.

Proper citation: RRID:Addgene_135657 Copy   

•   Source: Addgene

http://www.addgene.org/135658

Species: Synthetic
Genetic Insert: Intron containing lox site
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pAT19; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Erythromycin
References:
Comments: Use in combination with pQlox71F (addgene 135655) or pQlox71R (addgene 135656), then pQcre1 (addgene 135659). Transcription of LtrB-derived group II intron is driven by the pFer promoter. Redesign intron to target gene using TargeTron or Clostron algorithms (links below), then clone by SOE-PCR or gene synthesis ligation between NdeI and BsrGI sites. Lox66 and Lox71 must be in same orientation to delete the intervening sequence, but the directions of introns relative to one another can generate inverted repeats in the chromosome after deletion has taken place, leading to futher recombinations events. See publication for details. Intron redesign links http://www.clostron.com/clostron1.php http://www.targetrons.com/targetron_pLtrB.php Reference for intron redesign Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP. The ClosTron: A universal gene knock-out system for the genus Clostridium. J Microbiol Methods. 2007;70(3):452-464. Perutka J, Wang W, Goerlitz D, Lambowitz AM. Use of Computer-designed Group II Introns to Disrupt Escherichia coli DExH / D-box Protein and DNA Helicase Genes. 2004:421-439.

Proper citation: RRID:Addgene_135658 Copy   

•   Source: Addgene

http://www.addgene.org/135660

Species: Synthetic
Genetic Insert: Intron containing lox site
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pAT19; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Erythromycin
References:
Comments: Transcription of LtrB-derived group II intron is driven by the pFer promoter. Redesign intron to target gene using TargeTron or Clostron algorithms (links below), then clone by SOE-PCR or gene synthesis ligation between NdeI and BsrGI sites. Intron redesign links http://www.clostron.com/clostron1.php http://www.targetrons.com/targetron_pLtrB.php Reference for intron redesign Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP. The ClosTron: A universal gene knock-out system for the genus Clostridium. J Microbiol Methods. 2007;70(3):452-464. Perutka J, Wang W, Goerlitz D, Lambowitz AM. Use of Computer-designed Group II Introns to Disrupt Escherichia coli DExH / D-box Protein and DNA Helicase Genes. 2004:421-439.

Proper citation: RRID:Addgene_135660 Copy   

•   Source: Addgene

http://www.addgene.org/135661

Species: Synthetic
Genetic Insert: Intron containing lox site
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pAT19; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Erythromycin
References:
Comments: Transcription of LtrB-derived group II intron is driven by the pFer promoter. Redesign intron to target gene using TargeTron or Clostron algorithms (links below), then clone by SOE-PCR or gene synthesis ligation between NdeI and BsrGI sites. Intron redesign links http://www.clostron.com/clostron1.php http://www.targetrons.com/targetron_pLtrB.php Reference for intron redesign Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP. The ClosTron: A universal gene knock-out system for the genus Clostridium. J Microbiol Methods. 2007;70(3):452-464. Perutka J, Wang W, Goerlitz D, Lambowitz AM. Use of Computer-designed Group II Introns to Disrupt Escherichia coli DExH / D-box Protein and DNA Helicase Genes. 2004:421-439.

Proper citation: RRID:Addgene_135661 Copy   

•   Source: Addgene

http://www.addgene.org/46888

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:8539; Vector Backbone:pMSP3545; Vector Types:Bacterial Expression, Other, Nisin-inducible expression; Gram-positive Bacterial Shuttle Vector; Bacterial Resistance:Erythromycin
References:
Comments: This plasmid utilizes the NIsin Controlled Expression (NICE) system. NICE® is a registered trademark of NIZO food research BV, P.O.Box 20, 6710 BA Ede, The Netherlands. pMSP3545 contains the Nisin-inducible PnisA promoter, and the pAMB1 replicon for expression in gram-positive bacteria. It also contains a unique NcoI cleavage site immediately downstream of the nisA ribosomal binding sequence, which may be used for translational fusions, and a rho-independent terminator between the XbaI and XhoI sites of the polylinker. For protein expression in E. faecalis, induce with 10-25 ng/mL nisin (Sigma, N-5764). See associated article for instructions on how to make nisin solution. Addgene's quality control sequencing finds discrepancies with the depositor's provided sequence, but they are not thought to affect plasmid function.

Proper citation: RRID:Addgene_46888 Copy   

•   Source: Addgene

http://www.addgene.org/190481

Species: Other
Genetic Insert: Upstream & Downstream pyrE deletion region
Vector Backbone Description: Vector Backbone:pJB11; Vector Types:Other, E. coli / B. subtilis - C. difficile shuttle vector; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_190481 Copy   

•   Source: Addgene

http://www.addgene.org/203684

Species:
Genetic Insert: DsRed-Express
Vector Backbone Description: Vector Backbone:pNF8; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_203684 Copy   

•   Source: Addgene

http://www.addgene.org/27168

Species: Lactococcus lactis and synthetic construct
Genetic Insert: surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
Vector Backbone Description: Backbone Size:7493; Vector Backbone:pTRKH3; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_27168 Copy   

•   Source: Addgene

http://www.addgene.org/27169

Species: Enterococcus faecalis and synthetic construct
Genetic Insert: erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
Vector Backbone Description: Backbone Size:7493; Vector Backbone:pTRKH3; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_27169 Copy   

•   Source: Addgene

http://www.addgene.org/203961

Species:
Genetic Insert: Erythromycin Resistance Gene
Vector Backbone Description: Backbone Size:863; Vector Backbone:R6Kγ origin of replication and oriT; Vector Types:Plant Expression; Bacterial Resistance:Erythromycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.06.05.543168v1 for bioRxiv preprint.

Proper citation: RRID:Addgene_203961 Copy   

•   Source: Addgene

http://www.addgene.org/203683

Species:
Genetic Insert: HcRed
Vector Backbone Description: Vector Backbone:pNF8; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_203683 Copy   

•   Source: Addgene

http://www.addgene.org/191345

Species: Other
Genetic Insert: Plac-RFP (IPTG-inducible red fluorescent protein)
Vector Backbone Description: Backbone Marker:Chain Biotech; Backbone Size:4601; Vector Backbone:pMTL82241; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_191345 Copy   

•   Source: Addgene

http://www.addgene.org/191354

Species: Synthetic
Genetic Insert: Pcphy23-NanoLuc
Vector Backbone Description: Backbone Marker:PMID 19775243; Backbone Size:6972; Vector Backbone:pQexp; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_191354 Copy   

•   Source: Addgene

http://www.addgene.org/191351

Species: Other
Genetic Insert: Plac-RFP (IPTG-inducible red fluorescent protein)
Vector Backbone Description: Backbone Marker:Chain Biotech; Backbone Size:5644; Vector Backbone:pMTL84241; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_191351 Copy   

•   Source: Addgene

http://www.addgene.org/218508

Species: Synthetic
Genetic Insert: mNeongreen
Vector Backbone Description: Vector Backbone:p7INT; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments: This integrative plasmid enables expression of the fluorescence reporter mNeongreen in S. pyogenes. Due to a mutation in the SsrA degradation tag sequence (LAA to LDD), that is translationally fused to the mNeongreen protein, the reporter is destabilised and shows a reduced half-life compare to the untagged mNeongreen protein. The destabilised reporter is advantageous when gene expression is monitored over time as dynamic changes in gene expression (e.g. downregulation) can be observed. See the associated publication for more details. Resource Information: The mNeongreen gene was codon-optimised for expression in S. pyogenes and obtained by gene synthesis. The lactococcal P23 promoter was taken from pLZ12Km2-P23R:TA:ffluc (Plasmid #88900). p7INT was a gift from Prof. Michael Federle (University of Illinois Chicago, USA). The p7INT plasmid integrates into the 3' end of the tmRNA locus in several S. pyogenes strains. It was originally described in: McShan, W.M., McLaughlin, R.E., Nordstrand, A. et al. Vectors containing streptococcal bacteriophage integrases for site-specific gene insertion. Methods Cell Sci 20, 51–57 (1998). https://doi.org/10.1023/A:1009773309163 Please download the detailed plasmid map using the file linked below.

Proper citation: RRID:Addgene_218508 Copy   

•   Source: Addgene

http://www.addgene.org/209317

Species: Other
Genetic Insert: Group B Streptococcus-compatible single guide RNA
Vector Backbone Description: Vector Backbone:vpL3004; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_209317 Copy   

•   Source: Addgene

http://www.addgene.org/203814

Species: Other
Genetic Insert: Mini-Tn, aprE cassette
Vector Backbone Description: Vector Backbone:pKS1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Erythromycin
References:
Comments: Plasmid can also be transformed in Bacillus subtilis, grown at 37 C

Proper citation: RRID:Addgene_203814 Copy   

•   Source: Addgene

http://www.addgene.org/223203

Species:
Genetic Insert: N/A
Vector Backbone Description: Vector Backbone:pJC005.em; Vector Types:Other, Complementation Vector; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_223203 Copy   

•   Source: Addgene

http://www.addgene.org/223202

Species:
Genetic Insert: N/A
Vector Backbone Description: Vector Backbone:pJC005.em; Vector Types:Other, Complementation Vector; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_223202 Copy   

•   Source: Addgene

http://www.addgene.org/229146

Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Addgene ; Backbone Size:2800; Vector Backbone:pLp_3050sNuc; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
References:
Comments:

Proper citation: RRID:Addgene_229146 Copy   

•   Source: Addgene