Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Icon Genetics; Vector Backbone:Xpre-K (pICH50505) ; Vector Types:Other, plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54381 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-K (pCAMBIA); Vector Types:Other, plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54388 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Icon Genetics; Vector Backbone:Xpre-K (pICH50505) ; Vector Types:Other, plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54386 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Icon Genetics; Vector Backbone:Xpre-K (pICH50505) ; Vector Types:Other, plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54384 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-K (pCAMBIA); Vector Types:Other, plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54393 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-K (pCAMBIA); Vector Types:Other, plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54392 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-K (pCAMBIA); Vector Types:Other, plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54391 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Cambia; Vector Backbone:pCAMBIA1300; Vector Types:Other, Multisite Gateway; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: See genbank file for best annotation of features.
Proper citation: RRID:Addgene_71275 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Thermo; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/03/14/277350 for BioRxiv preprint
Proper citation: RRID:Addgene_71534 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9000; Vector Backbone:pBI101; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Aoyama, T. and Chua, N.-H. (1997) A glucocorticoid-mediated
transcriptional induction system in transgenic plants. Plant J.
11, 605–612
Proper citation: RRID:Addgene_71745 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Please note that parental strain (MC4100) is streptomycin-resistant.
Proper citation: RRID:Addgene_69771 Copy
Species: Other
Genetic Insert: OsMIR390-B/c
Vector Backbone Description: Backbone Size:9620; Vector Backbone:pMDC123; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_61466 Copy
Species: Other
Genetic Insert: ndmA
Vector Backbone Description: Backbone Marker:igem parts registry; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: The sequence of this plasmid is updated from the GenBank entry for pDCAF3 (KC619530.1) to include an IS5 insertion that occurred in the promoter region of the caffeine demethylation genes during passaging of the strain and a base change in the sequence of the gst9 gene. This plasmid still functions as described in the publication for pDCAF3.
Vector constitutively expresses N-demethylase genes under the control of the BBa_J23100 promoter (http://parts.igem.org/Part:BBa_J23100).
N-demethylases are only functional when grown at 30°C and require iron supplementation in minimal media. See:
Summers, R. M., Louie, T. M., Yu, C.-L., Gakhar, L., Louie, K.C., and Subramanian, M. (2012) Novel, highly specific Ndemethylases enable bacteria to live on caffeine and related purine alkaloids. J. Bacteriol. 194, 2041−2049.
For the quantification of caffeine (or other methylxanthine) content it is recommended to grow cells in M9 minimal medium supplemented with 2 g/L glucose and 2 g/L casein (M9GC).
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at help@addgene.org or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_65220 Copy
Species: Synthetic
Genetic Insert: Splitposon w/ KanR, LacI & PTac
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_59947 Copy
Species: Other
Genetic Insert: monomeric red fluorescent protein
Vector Backbone Description: Backbone Marker:Cambia, Canberra, Australia; Backbone Size:6000; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_61649 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_61703 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMDC32; Vector Types:Plant Expression, Other, Gateway compatible attR1-attR2 destination vector; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Note: Addgene's quality control sequencing finds a 1.2kb transposable element upstream of the Kanamycin resistance cassette. This inserted sequence does not affect plasmid function.
Proper citation: RRID:Addgene_86207 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pK18; Vector Types:Bacterial Expression, Cre/Lox, Other, Herpesvirus Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: The BAC vector is derived from the E.coli F plasmid. The BAC vector was cloned into the high copy vector pK18, to increase yields. The BAC vector is typically excised by PacI. The BAC vector contains genes for participation (sopA, B,C, a repE gene (required for replication) and an origin of replication (oriS). There is also a chloramphenicol resistance gene. The BAC vector carries a gpt gene, driven by the HSV tk promoter; the gpt gene is followed by the SV40 polyA signal sequence (ca 125 bp). The BAC vector is terminally flanked by loxP sites. The plasmid contains also a 1.5 kb EcoRI-fragment from the murine herpesvirus MHV68. The sequence of pK18 is available from Genbank ACCESSION M17626.
Proper citation: RRID:Addgene_85709 Copy
Species: Arabidopsis thaliana
Genetic Insert: STTM165/166
Vector Backbone Description: Backbone Marker:Guiliang Tang (Addgene plasmid # 44182); Backbone Size:8608; Vector Backbone:pFGC5941-PacI; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR.
Proper citation: RRID:Addgene_84091 Copy
Species: Arabidopsis thaliana
Genetic Insert: STTM169a
Vector Backbone Description: Backbone Marker:Guiliang Tang (Addgene plasmid # 44182); Backbone Size:8608; Vector Backbone:pFGC5941-PacI; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR. The STTM sequence in the plasmid contains discrepancies compared to the reference sequence. According to the depositing lab, these mutations should not affect plasmid function.
Proper citation: RRID:Addgene_84094 Copy
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