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http://www.addgene.org/13588

Species: Homo sapiens
Genetic Insert: integrin alpha 9 / alpha 5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: See article for cloning details.

Proper citation: RRID:Addgene_13588 Copy   

•   Source: Addgene

http://www.addgene.org/13585

Species: Homo sapiens
Genetic Insert: integrin alpha 9
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: Please note that the first 41 amino acids of the integrin alpha insert show greater identity to integrin alpha-5 rather than integrin alpha-9

Proper citation: RRID:Addgene_13585 Copy   

•   Source: Addgene

http://www.addgene.org/13582

Species: Homo sapiens
Genetic Insert: integrin beta 6 777T
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_13582 Copy   

•   Source: Addgene

http://www.addgene.org/17841

Species: Mus musculus
Genetic Insert: GlyCAM-1/IgG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_17841 Copy   

•   Source: Addgene

http://www.addgene.org/170473

Species: Synthetic
Genetic Insert: T7A1, O-, O1, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: LacI operators separated by 400 base pairs.

Proper citation: RRID:Addgene_170473 Copy   

•   Source: Addgene

http://www.addgene.org/170471

Species: Synthetic
Genetic Insert: T7A1, Os, O2, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: LacI operators separated by 400 base pairs.

Proper citation: RRID:Addgene_170471 Copy   

•   Source: Addgene

http://www.addgene.org/170477

Species: Synthetic
Genetic Insert: T7A1, O2, O1, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: LacI operators separated by 400 base pairs.

Proper citation: RRID:Addgene_170477 Copy   

•   Source: Addgene

http://www.addgene.org/170478

Species: Synthetic
Genetic Insert: T7A1, O2, Os, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: LacI operators separated by 400 base pairs.

Proper citation: RRID:Addgene_170478 Copy   

•   Source: Addgene

http://www.addgene.org/154035

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Other, Diatom cloning; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: selectable marker added for centric diatoms

Proper citation: RRID:Addgene_154035 Copy   

•   Source: Addgene

http://www.addgene.org/24502

Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: This is Gq alpha with an HA epitope engineered into an internal site that does not seem to affect receptor coupling in multiple studies. Epitope tagged by Paul Wilson, see Wedegaertner, JBC 268: 25001-25008. The 5' non-coding sequences were removed, but the 3' non-coding sequences remain, as in Strathmann & Simon (1990) PNAS 87:9113-9117. The parent construct has been donated to the ATCC by the Bourne Lab. There is a 1bp insertion, a 5bp insertion, and a 1bp deletion when author sequence is aligned with Addgene quality control sequence. These differences are between the stop codon and the SP6 sequence. Please see supplemental document for G-protein chimera user manual.

Proper citation: RRID:Addgene_24502 Copy   

•   Source: Addgene

http://www.addgene.org/42600

Species: Caenorhabditis elegans
Genetic Insert: let-363 cDNA
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: This plasmid can be kept in DH5alpha cells but does not work for RNAi in that strain. For RNAi, please transform DNA into the HT115 strain.

Proper citation: RRID:Addgene_42600 Copy   

•   Source: Addgene

http://www.addgene.org/42601

Species: Caenorhabditis elegans
Genetic Insert: daf-15
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: This plasmid can be kept in DH5alpha cells but does not work for RNAi in that strain. For RNAi, please transform DNA into the HT115 strain. Note: Addgene's quality control sequencing has found that the deposited clone contains a T to C mismatch with respect to the C. elegans N2 reference daf-15 sequence, which would cause a V797A change in the coded protein. According to the depositing lab, this point mutation does not affect RNAi feeding; however, its possible effects in other contexts are not known.

Proper citation: RRID:Addgene_42601 Copy   

•   Source: Addgene

http://www.addgene.org/32697

Species: Homo sapiens
Genetic Insert: RAP1
Vector Backbone Description: Backbone Marker:Anumanthan et al. 1998; Vector Backbone:pAXEF-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_32697 Copy   

•   Source: Addgene

http://www.addgene.org/64946

Species:
Genetic Insert: Flp recombinase
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pFLP1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: GenBank Accession # AY597273 Compared to the Genbank reference sequence AY597273, Addgene's sequencing results found a T350I mutation in the tetracycline cassette and a G5D mutation in FLP recombinase. The effect of these differences is unknown.

Proper citation: RRID:Addgene_64946 Copy   

•   Source: Addgene

http://www.addgene.org/64951

Species:
Genetic Insert: FRT-TcR-FRT
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pFRT2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: GenBank Accession #AY712950 Compared to the Genbank reference sequence AY712950, Addgene's sequencing results found a T350I mutation in the tetracycline cassette. The effect of this difference is unknown.

Proper citation: RRID:Addgene_64951 Copy   

•   Source: Addgene

http://www.addgene.org/74110

Species:
Genetic Insert: tetA(C)
Vector Backbone Description: Backbone Marker:Alexeyev, M.F., BioTechniques 18:52-56, 1995; Vector Backbone:pBSL15; Vector Types:Other, Bacterial vector based on pMB1 replicon; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_74110 Copy   

•   Source: Addgene

http://www.addgene.org/74104

Species: Synthetic
Genetic Insert: tetA(C)
Vector Backbone Description: Vector Backbone:pUCBM21; Vector Types:Other, Conjugative bacterial vector; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_74104 Copy   

•   Source: Addgene

http://www.addgene.org/67273

Species:
Genetic Insert: tetA
Vector Backbone Description: Backbone Size:8754; Vector Backbone:pMJH46; Vector Types:Bacterial Expression, Other, Red Recombinase, temperature sensitive; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: Conjugally transferable red recombinase vector pMJH65

Proper citation: RRID:Addgene_67273 Copy   

•   Source: Addgene

http://www.addgene.org/80388

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4360; Vector Backbone:pBR322; Vector Types:Synthetic Biology, Other, ; Episomal vector for Phaeodactylum tricornutum; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_80388 Copy   

•   Source: Addgene

http://www.addgene.org/8633

Species: Homo sapiens
Genetic Insert: Human p150,95
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: This clone will direct the expression of functional p150,95 upon cotransfection with the beta2 subunit in a variety of cell types. This sequence has been deposited in Genbank with accession number Y00093. Also see refs: Nature 329, 840-842 (1987) and J of Immunology 146, 648-655 (1991).

Proper citation: RRID:Addgene_8633 Copy   

•   Source: Addgene