| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
5TTW (EED) Resource Report Resource Website |
RRID:Addgene_101254 | EED | Homo sapiens | Kanamycin | PMID: | N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: EED:APC049-D10:C27444. SGC or PDB link: http://www.thesgc.org/structures/5TTW/ | Backbone Marker:SGC (Addgene Plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:03 | 0 | |
|
pSNAP-tag (m) Vector Resource Report Resource Website |
RRID:Addgene_101135 | Ampicillin | PMID: | Codon usage of the gene is optimized for expression in mammalian cells. pSNAP-tag(m) contains two multiple cloning sites to allow cloning of the fusion partner as a fusion to the N- or C-terminus of the SNAP-tag. to allow cloning of the fusion partner as a fusion to the N- or C-terminus of the SNAP-tag. For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/ This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com. | Backbone Size:5802; Vector Backbone:pSNAP-tag (m) Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:02 | 0 | |||
|
pSNAP-tag (T7) Vector Resource Report Resource Website |
RRID:Addgene_101137 | Ampicillin | PMID: | pSNAP-tag(T7) is a high copy number E. coli expression plasmid encoding SNAP26b, a SNAP-tag protein, which can be expressed under the control of the T7 promoter. Codon usage of the gene is optimized for expression in E. coli. The fusion partner should be cloned as a fusion to the C-terminus of the SNAP-tag. For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/ This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com. | Backbone Size:3725; Vector Backbone:pSNAP-tag (T7) Vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:02 | 0 | |||
|
pDD2002 Resource Report Resource Website |
RRID:Addgene_101198 | LANApi driving green luciferase reporter for CBG68 in pCBG68 backbone. | Other | Ampicillin | PMID:22740392 | Backbone Marker:Promega; Backbone Size:5104; Vector Backbone:pCBG68 Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:02 | 0 | ||
|
BPK4393 - human expression plasmid for SpCas9 Cluster 5 + Q926A Resource Report Resource Website |
RRID:Addgene_101192 | hSpCas9-Cluster5+Q926A(K918A/V922A/R925A/Q926A) | Synthetic | Ampicillin | PMID:28931002 | Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | K918A/V922A/R925A/Q926A | 2022-04-22 03:15:02 | 0 | |
|
pRSETb-ffDronpa-F Resource Report Resource Website |
RRID:Addgene_101193 | ffDronpa-F | Ampicillin | PMID:28561832 | Backbone Size:2860; Vector Backbone:pRSETb; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | K45I, V60A, F173V | 2022-04-22 03:15:02 | 0 | ||
|
pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant Resource Report Resource Website |
RRID:Addgene_101233 | SpCas9 variant G582A/V583A/E584A/D585A/N588A | Other | Ampicillin | PMID:28931002 | Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. | Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | G582A, V583A, E584A, D585A and N588A | 2022-04-22 03:15:03 | 0 |
|
pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant Resource Report Resource Website |
RRID:Addgene_101235 | SpCas9 variant F491A/M495A/T496A/N497A/Q926A | Other | Ampicillin | PMID:28931002 | Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. | Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | F491A, M495A, T496A, N497A and Q926A | 2022-04-22 03:15:03 | 0 |
|
Opto-CRAC version 1 Resource Report Resource Website |
RRID:Addgene_101245 | LOV2 and STIM1 cytosolic fragment | Synthetic | Ampicillin | PMID:26646180 | Backbone Size:5000; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:03 | 0 | ||
|
aCat TL2 (1-697 mTFP1-TSmod-Venus STOP) Resource Report Resource Website |
RRID:Addgene_101298 | alpha-Catenin | Mus musculus | Kanamycin | PMID:28329679 | The TS module with mTFP1-F40-venusA206K was adapted from Vinculin TS (a kind gift from Martin Schwartz [Grashoff et al., 2010]). The TSmod was cloned in to pEGFP-N1 vector at Xho1 and Not1 restriction sites. For aE-Cat-TS, the mouse N-terminal (1_697 aa) of alpha-catenin was inserted before mTFP1 by in- fusion cloning (Takara Bioscience, Clontech) at the Xho1 restriction site | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Contains amino acids 1-698 of alpha-Catenin | 2022-04-22 03:15:04 | 0 |
|
aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907) Resource Report Resource Website |
RRID:Addgene_101299 | alpha-Catenin | Mus musculus | Kanamycin | PMID:28329679 | The C-terminal fragment (698-906 aa) of alpha-catenin was cloned after VenusA206K at the Not1 restriction site | Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Contains amino acids 1-698 and 698-906 of alpha-Catenin | 2022-04-22 03:15:04 | 0 |
|
5WCJ (METTL13) Resource Report Resource Website |
RRID:Addgene_101270 | METTL13 | Homo sapiens | Ampicillin | PMID: | N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: METTL13:PBC007-B09:C235684. SGC or PDB link: http://www.thesgc.org/structures/5WCJ/ | Backbone Marker:SGC (Addgene Plasmid #62304); Vector Backbone:pFBOH-MHL; Vector Types:Other, Baculovirus Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:04 | 0 | |
|
2QO2 (EPHA3) Resource Report Resource Website |
RRID:Addgene_101271 | EPHA3 | Homo sapiens | Kanamycin | PMID: | N terminal tag: MGSSHHHHHHSSGLVPRGS. SGC Clone Sample ID: EPHA3:SDC033-F08:C12121. SGC or PDB link: http://www.rcsb.org/pdb/explore/explore.do?structureId=2QO2 | Backbone Marker:SGC (Addgene Plasmid #26094); Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:04 | 0 | |
|
4PY6 Resource Report Resource Website |
RRID:Addgene_101272 | 4PY6 | Other | Ampicillin | PMID: | N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: PFA0510w:MAC02W-B09:C201930. SGC or PDB link: http://www.rcsb.org/pdb/explore/explore.do?structureId=4PY6 | Backbone Marker:SGC (Addgene Plasmid #26092); Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:04 | 0 | |
|
pMflST-o4 Resource Report Resource Website |
RRID:Addgene_101316 | oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA) | Other | Tetracycline | PMID:28115382 | Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline | 2022-04-22 03:15:05 | 0 | ||
|
pMflT-o2 Resource Report Resource Website |
RRID:Addgene_101310 | oriC2 of M. florum strain L1 (dnaA/dnaN intergenic region) | Other | Tetracycline | PMID:28115382 | Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline | 2022-04-22 03:15:04 | 0 | ||
|
pMflT-o3 Resource Report Resource Website |
RRID:Addgene_101311 | oriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA) | Other | Tetracycline | PMID:28115382 | Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline | 2022-04-22 03:15:04 | 0 | ||
|
pCS2.0 Tag-RFP-T-UtrCH Resource Report Resource Website |
RRID:Addgene_101279 | Utrophin | Homo sapiens | Ampicillin | PMID:24413434 | Vector Backbone:pCS2.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:04 | 0 | ||
|
pDD2029 Resource Report Resource Website |
RRID:Addgene_101372 | LANApi TATA box mutation in pBluescript-LANApi,K14 (pDD2000). | Other | Ampicillin | PMID:22740392 | Addgene's sequencing found Y108H and G360D mutations in CBG68luc. These mutations are not known to affect plasmid function. | Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | LANApi TATA box mutation in pBluescript-LANApi,K14 (pDD2000). | 2022-04-22 03:15:05 | 0 |
|
pDD2030 Resource Report Resource Website |
RRID:Addgene_101373 | RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000). | Other | Ampicillin | PMID:22740392 | Addgene's sequencing found V466A and R510C mutations in CBG68luc and a V298A mutation in CBRluc. These mutations are not known to affect plasmid function. | Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000). | 2022-04-22 03:15:06 | 0 |
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