Species: Synthetic
Genetic Insert: hSpCas9-Cluster5(K918A/V922A/R925A)
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101191 Copy
Species: Synthetic
Genetic Insert: hSpCas9-Cluster3(T657A/G658A/W659A/R661A)
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101187 Copy
Species: Other
Genetic Insert: SpCas9 variant N692A/M694A/Q695A/H698A/Q926A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101220 Copy
Species: Synthetic
Genetic Insert: hSpCas9-Cluster3+Q926A(T657A/G658A/W659A/R661A/Q926A)
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101188 Copy
Species: Synthetic
Genetic Insert: hSpCas9-Cluster1Q695+Q926A(N692A/M694A/H698A/Q926A)
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101181 Copy
Species: Synthetic
Genetic Insert: hSpCas9-Cluster2+Q926A(G582A/V583A/E584A/D585A/N588A/Q926A)
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101184 Copy
Species: Other
Genetic Insert: SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101229 Copy
Species: Synthetic
Genetic Insert: hSpCas9-Cluster4(F491A/M495A/T496A/N497A)
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101189 Copy
Species: Other
Genetic Insert: SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101208 Copy
Species: Other
Genetic Insert: SpCas9 variant N497A/R661A/Q695A/Q926A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101209 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HH-gRNA-HDV targetting SeILV6 in S. pastorianus
Vector Backbone Description: Backbone Size:5868; Vector Backbone:pUDP003; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101166 Copy
Species: Other
Genetic Insert: SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101204 Copy
Species: Other
Genetic Insert: SpCas9 variant Q926A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101206 Copy
Species: Other
Genetic Insert: SpCas9 variant N497A/R661A/Q695A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101207 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HH-gRNA-HDV targetting SeILV6 in S. pastorianus
Vector Backbone Description: Backbone Marker:JM Daran; Backbone Size:10664; Vector Backbone:pUDP004; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101167 Copy
Species: Other
Genetic Insert: SpCas9 variant C80S/C574S/E60C/D273C
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101200 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
Vector Backbone Description: Backbone Marker:JM Daran; Backbone Size:10664; Vector Backbone:pUDP004; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101168 Copy
Species: Other
Genetic Insert: SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101203 Copy
Species: Homo sapiens
Genetic Insert: Cox8A
Vector Backbone Description: Backbone Size:6077; Vector Backbone:pCLIP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Originally cloned by Covalys. The CLIP-tag is an C-insert tag. Additional antibiotic resistance to Kanmycin
For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
Proper citation: RRID:Addgene_101130 Copy
Species: Other
Genetic Insert: GPI
Vector Backbone Description: Backbone Size:5151; Vector Backbone:pMCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Originally cloned by Covalys. The GPI only is 87 bp. The size of ER signal sequence + MCP+GPI is 408 bp. The MCP-tag is N-terminal to the GPI sequence. Additional resistance to Kanamycin.
For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
Proper citation: RRID:Addgene_101132 Copy
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