Species: Rattus norvegicus
Genetic Insert: trkB.T1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5591; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121502 Copy
Species: Rattus norvegicus
Genetic Insert: IGFBP1 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3 basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_12146 Copy
Species: Rattus norvegicus
Genetic Insert: neurotensin receptor
Vector Backbone Description: Backbone Size:6991; Vector Backbone:pRG/III-hs-MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Internal plasmid code # 624
Proper citation: RRID:Addgene_121677 Copy
Species: Rattus norvegicus
Genetic Insert: CGR
Vector Backbone Description: Backbone Marker:atcc; Backbone Size:8400; Vector Backbone:marked pRS423; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Derived from plasmids from the following reference: Picard D, Khurseed B, Garabedian MJ, Fortin MG, Lindquist S, Yamamoto
KR. Reduced levels of hsp90 compromise steroid receptor action in
vivo. Nature (1990), 348 (6297): 166-8.
Proper citation: RRID:Addgene_1218 Copy
Species: Rattus norvegicus
Genetic Insert: SEPT11
Vector Backbone Description: Vector Backbone:pCDNA 3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_122022 Copy
Species: Rattus norvegicus
Genetic Insert: SEPT11 shRNA
Vector Backbone Description: Vector Backbone:mU6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments: 3-point mutations (negative control for SEPT11 sh2 shRNA targeting Septin 11 mRNA, plasmid 122023).
Proper citation: RRID:Addgene_122024 Copy
Species: Rattus norvegicus
Genetic Insert: GR
Vector Backbone Description: Backbone Marker:atcc; Backbone Size:9200; Vector Backbone:pRS423; Vector Types:Yeast Expression, Other, 2micron; Bacterial Resistance:Ampicillin
References:
Comments: Derived from plasmids from the following reference: Picard D, Khurseed B, Garabedian MJ, Fortin MG, Lindquist S, Yamamoto
KR. Reduced levels of hsp90 compromise steroid receptor action in
vivo. Nature (1990), 348 (6297): 166-8.
Proper citation: RRID:Addgene_1220 Copy
Species: Rattus norvegicus
Genetic Insert: angiotensinogen
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5200; Vector Backbone:pRC/RSV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_122104 Copy
Species: Rattus norvegicus
Genetic Insert: fRCaMP2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3703; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: fRCaMP2 is the D396A variant of the jRCaMP1a gene cloned from the pGP-CMV vector (#61563) published by Dana et al. 2016.
Proper citation: RRID:Addgene_125246 Copy
Species: Rattus norvegicus
Genetic Insert: fRCaMP2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3955; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: fRCaMP2 is the D396A variant of the jRCaMP1a gene cloned from the pGP-CMV vector (#61563) published by Dana et al. 2016.
Proper citation: RRID:Addgene_125250 Copy
Species: Rattus norvegicus
Genetic Insert: CCAAT/Enhancer-binding Protein beta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The C/EBPbeta gene encodes 3 in-frame translational isoforms (LAP*, LAP, and LIP). LAP is the predominant activating isoform expressed in most cells, and this plasmid is designed to express the LAP isoform of the protein. The construct does not include the extra 21 codons at the N terminus that are present in LAP*. The complete insert (not just the ORF) includes a Kozak box sequence at the N terminus.
For more information on the C/EBPbeta isoforms, please see the original reference:
Descombes, P., and Schibler, U. A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67:569-579 (1991).
Proper citation: RRID:Addgene_12558 Copy
Species: Rattus norvegicus
Genetic Insert: Cx 26 (rat)
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 3b parts are used for coding sequences in the MTK. Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123796 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1-miRFP670nano
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_127435 Copy
Species: Rattus norvegicus
Genetic Insert: PCDHGC5
Vector Backbone Description: Vector Backbone:pCDNA 3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert was originally cloned in Angel de Blas's lab.
Proper citation: RRID:Addgene_122229 Copy
Species: Rattus norvegicus
Genetic Insert: PCDHGC5 shRNA
Vector Backbone Description: Vector Backbone:mU6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments: Insert was originally cloned in Angel de Blas's lab.
Proper citation: RRID:Addgene_122228 Copy
Species: Rattus norvegicus
Genetic Insert: PCDHGC5 shRNA
Vector Backbone Description: Vector Backbone:mU6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments: Insert was originally cloned in Angel de Blas's lab.
Proper citation: RRID:Addgene_122227 Copy
Species: Rattus norvegicus
Genetic Insert: GABRG2 shRNA
Vector Backbone Description: Vector Backbone:mU6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments: It targets gamma2 GABAA receptor subunit mRNA, nucleotides 130–155 located in the protein coding region of GenBank accesion number L08497. It targets both gamma2S and gamma2L mRNAs).
Proper citation: RRID:Addgene_120794 Copy
Species: Rattus norvegicus
Genetic Insert: GABRG2 shRNA
Vector Backbone Description: Vector Backbone:mU6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments: 3-point mutations (negative control for shRNA2 targeting gamma2 mRNA, plasmid 120794).
Proper citation: RRID:Addgene_120795 Copy
Species: Rattus norvegicus
Genetic Insert: GABRG2 shRNA
Vector Backbone Description: Vector Backbone:mU6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments: It targets gamma2 GABAA receptor subunit mRNA, nucleotides 1467–1491 located in the 3'-UTR of GenBank accesion number L08497. It targets both gamma2S and gamma2L mRNAs.
Proper citation: RRID:Addgene_120796 Copy
Species: Rattus norvegicus
Genetic Insert: sup35 NM domain fused with GR
Vector Backbone Description: Backbone Size:4847; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Derived from plasmids from the following reference: Picard D, Khurseed B, Garabedian MJ, Fortin MG, Lindquist S, Yamamoto
KR. Reduced levels of hsp90 compromise steroid receptor action in
vivo. Nature (1990), 348 (6297): 166-8.
Proper citation: RRID:Addgene_1211 Copy
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